Biomimetic Hydrogels as the Inductive Endochondral Ossification Template for Promoting bone Regeneration DOI
Fujian Zhao, Yonghao Qiu, Wenjing Liu

и другие.

Advanced Healthcare Materials, Год журнала: 2023, Номер 13(18)

Опубликована: Дек. 18, 2023

Repairing critical size bone defects (CSBD) is a major clinical challenge and requires effective intervention by biomaterial scaffolds. Inspired the fact that cartilaginous template-based endochondral ossification (ECO) process crucial to healing development, developing biomimetic biomaterials promote ECO recognized as promising approach for repairing CSBD. With unique highly hydrated 3D polymeric network, hydrogels can be designed closely emulate physiochemical properties of cartilage matrix facilitate ECO. In this review, various preparation methods possessing specific required promoting are introduced. The materiobiological impacts physicochemical hydrogels, such mechanical properties, topographical structures chemical compositions on ECO, associated molecular mechanisms related BMP, Wnt, TGF-β, HIF-1α, FGF, RhoA signaling pathways further summarized. This review provides detailed coverage insights design hydrogel-based regeneration.

Язык: Английский

Volumetric Printing Across Melt Electrowritten Scaffolds Fabricates Multi‐Material Living Constructs with Tunable Architecture and Mechanics DOI Creative Commons
Gabriel Größbacher, Michael Bartolf‐Kopp, Csaba Gergely

и другие.

Advanced Materials, Год журнала: 2023, Номер 35(32)

Опубликована: Апрель 26, 2023

Major challenges in biofabrication revolve around capturing the complex, hierarchical composition of native tissues. However, individual 3D printing techniques have limited capacity to produce composite biomaterials with multi-scale resolution. Volumetric bioprinting recently emerged as a paradigm-shift biofabrication. This ultrafast, light-based technique sculpts cell-laden hydrogel bioresins into structures layerless fashion, providing enhanced design freedom over conventional bioprinting. it yields prints low mechanical stability, since soft, cell-friendly hydrogels are used. Herein, possibility converge volumetric melt electrowriting, which excels at patterning microfibers, is shown for fabrication tubular hydrogel-based composites behavior. Despite including non-transparent electrowritten scaffolds process, high-resolution bioprinted successfully achieved. Tensile, burst, and bending properties printed tubes tuned altering mesh design, resulting multi-material constructs customizable, anisotropic geometries that better mimic intricate biological structures. As proof-of-concept, engineered obtained by building trilayered vessels, features (valves, branches, fenestrations) can be rapidly using this hybrid approach. multi-technology convergence offers new toolbox manufacturing mechanically tunable living

Язык: Английский

Процитировано

61
Progress in the application of sustained-release drug microspheres in tissue engineering DOI Creative Commons

Lian Ruan,

Mengrong Su,

Xinyun Qin

и другие.

Materials Today Bio, Год журнала: 2022, Номер 16, С. 100394 - 100394

Опубликована: Авг. 13, 2022

Sustained-release drug-loaded microspheres provide a long-acting sustained release, with targeted and other effects. There are many types of sustained-release drug various preparation methods, they easy to operate. For these reasons, have attracted widespread interest widely used in tissue engineering fields. In this paper, we systematic review the application engineering. First, introduce new type delivery system (sustained-release carriers), describe microspheres, summarize characteristics different microspheres. Second, methods materials required for preparing Third, applications summarized. Finally, shortcomings discuss future prospects development The purpose paper was further understanding personnel engaged related fields inspiration ideas studies

Язык: Английский

Процитировано

65
Collagen for neural tissue engineering: Materials, strategies, and challenges DOI Creative Commons
Wenhui Huang, Shenglong Ding,

Xi-Yuan Zhao

и другие.

Materials Today Bio, Год журнала: 2023, Номер 20, С. 100639 - 100639

Опубликована: Май 2, 2023

Neural tissue engineering (NTE) has made remarkable strides in recent years and holds great promise for treating several devastating neurological disorders. Selecting optimal scaffolding material is crucial NET design strategies that enable neural non-neural cell differentiation axonal growth. Collagen extensively employed NTE applications due to the inherent resistance of nervous system against regeneration, functionalized with neurotrophic factors, antagonists growth inhibitors, other growth-promoting agents. Recent advancements integrating collagen manufacturing strategies, such as scaffolding, electrospinning, 3D bioprinting, provide localized trophic support, guide alignment, protect cells from immune activity. This review categorises analyses collagen-based processing techniques investigated neural-specific applications, highlighting their strengths weaknesses repair, recovery. We also evaluate potential prospects challenges using biomaterials NTE. Overall, this offers a comprehensive systematic framework rational evaluation

Язык: Английский

Процитировано

37
Photocrosslinked methacrylated natural macromolecular hydrogels for tissue engineering: A review DOI

Xiaoli Yang,

Xiaojing Li,

Zhaoping Wu

и другие.

International Journal of Biological Macromolecules, Год журнала: 2023, Номер 246, С. 125570 - 125570

Опубликована: Июнь 25, 2023

Язык: Английский

Процитировано

33
Stem Cells Expansion Vector via Bioadhesive Porous Microspheres for Accelerating Articular Cartilage Regeneration DOI
Lang Bai, Qibin Han, Zeyu Han

и другие.

Advanced Healthcare Materials, Год журнала: 2023, Номер 13(3)

Опубликована: Ноя. 10, 2023

Abstract Stem cell tissue engineering is a potential treatment for osteoarthritis. However, the number of stem cells that can be delivered, loss during injection, and migration ability limit applications traditional engineering. Herein, kartogenin (KGN)‐loaded poly(lactic ‐co‐ glycolic acid) (PLGA) porous microspheres first engineered via emulsification, then anchored with chitosan through amidation reaction to develop new microsphere (PLGA‐CS@KGN) as expansion vector. Following 3D co‐culture PLGA‐CS@KGN carrier mesenchymal (MSCs), delivery system injected into capsule cavity in situ. In vivo vitro experiments show PLGA‐CS have high cell‐carrying capacity up 1 × 10 4 mm −3 provide effective protection MSCs promote their controlled release osteoarthritis microenvironment. Simultaneously, KGN loaded inside effectively cooperated induce differentiate chondrocytes. Overall, these findings indicate held cell‐loading ability, adapt cells, express markers associated cartilage repair. Thus, used enhancing therapy treatment.

Язык: Английский

Процитировано

26
In Situ Remodeling of Efferocytosis via Lesion‐Localized Microspheres to Reverse Cartilage Senescence DOI Creative Commons
Wei Xiong, Zeyu Han, Shenglong Ding

и другие.

Advanced Science, Год журнала: 2024, Номер 11(19)

Опубликована: Март 13, 2024

Abstract Efferocytosis, an intrinsic regulatory mechanism to eliminate apoptotic cells, will be suppressed due the delayed apoptosis process in aging‐related diseases, such as osteoarthritis (OA). In this study, cartilage lesion‐localized hydrogel microspheres are developed remodel situ efferocytosis reverse senescence and recruit endogenous stem cells accelerate repair. Specifically, aldehyde‐ methacrylic anhydride (MA)‐modified hyaluronic acid (AHM), loaded with pro‐apoptotic liposomes (liposomes encapsulating ABT263, A‐Lipo) PDGF‐BB, namely A‐Lipo/PAHM, prepared by microfluidic photo‐cross‐linking techniques. By a degraded porcine explant OA model, lesion location experiment illustrated that aldehyde‐functionalized promote affinity for cartilage. vitro data showed A‐Lipo induced of senescent chondrocytes (Sn‐chondrocytes), which can then phagocytosed macrophages, remodeling facilitated protection normal maintained chondrogenic differentiation capacity MSCs. vivo experiments confirmed localized reversed promoted repair OA. It is believed strategy great significance tissue regeneration diseases.

Язык: Английский

Процитировано

16
Injectable chitosan microspheres resisting inflammatory and oxidative stress for ameliorating intervertebral disc degeneration DOI
Lin Huang,

Wantao Wang,

Lei Liu

и другие.

Science China Materials, Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

Язык: Английский

Процитировано

1
Oxygen metabolism-balanced engineered hydrogel microspheres promote the regeneration of the nucleus pulposus by inhibiting acid-sensitive complexes DOI Creative Commons
Ziang Li, Feng Cai, Jincheng Tang

и другие.

Bioactive Materials, Год журнала: 2022, Номер 24, С. 346 - 360

Опубликована: Дек. 30, 2022

Intervertebral disc degeneration (IVDD) is commonly caused by imbalanced oxygen metabolism-triggered inflammation. Overcoming the shortcomings of antioxidants in IVDD treatment, including instability and lack targeting, remains challenging. Microfluidic surface modification technologies were combined to graft chitosan nanoparticles encapsulated with strong reductive black phosphorus quantum dots (BPQDs) onto GelMA microspheres via amide bonds construct metabolism-balanced engineered hydrogel (GM@CS-BP), which attenuate extracellular acidosis nucleus pulposus (NP), block inflammatory cascade, reduce matrix metalloproteinase expression (MMP), remodel (ECM) intervertebral discs (IVDs). The GM@CS-BP H2O2 intensity 229%. Chemical grafting electrostatic attraction increase encapsulation rate BPQDs 167% maintain stable release for 21 days, demonstrating antioxidant properties sustained modulation BPQDs. After western blotting revealed decreased acid-sensitive ion channel-3 factors. Histological staining an 8-week model confirmed regeneration NP. therefore a balance between ECM synthesis degradation regulating positive feedback metabolism IVDs This study provides in-depth interpretation mechanisms underlying antioxidation new approach treatment.

Язык: Английский

Процитировано

29
Architecture‐Engineered Electrospinning Cascade Regulates Spinal Microenvironment to Promote Nerve Regeneration DOI Creative Commons
Yu Tang, Zonghan Xu, Jincheng Tang

и другие.

Advanced Healthcare Materials, Год журнала: 2023, Номер 12(12)

Опубликована: Янв. 18, 2023

The inflammatory cascade after spinal cord injury (SCI) causes necrotizing apoptosis of local stem cells, which limits nerve regeneration. Therefore, coordinating the immune response and neural cell (NSC) functions is key to promoting recovery central nervous system function. In this study, a hydrogel "perfusion" electrospinning technology are integrated, "concrete" composite support for repair injuries built. hydrogel's hydrophilic properties activate macrophage integrin receptors mediate polarization into anti-inflammatory subtypes cause 10% increase in polarized M2 macrophages, thus reprogramming SCI microenvironment. Programmed stromal cell-derived factor-1α brain-derived neurotrophic factor released from recruitment neuronal differentiation NSCs by approximately four- twofold, respectively. fiber regulates microenvironment, recruits endogenous NSCs, promotes blood vessel germination maturation, improves function rat model. conclusion, engineering response. It regeneration through programmed cytokine-delivery system, further supplements mechanism regulated inherent biomaterial. new may serve as treatment approach SCI.

Язык: Английский

Процитировано

23
Recent advances in 3D bioprinted cartilage-mimicking constructs for applications in tissue engineering DOI Creative Commons
Jian Zhou, Qi Li, Zhuang Tian

и другие.

Materials Today Bio, Год журнала: 2023, Номер 23, С. 100870 - 100870

Опубликована: Ноя. 17, 2023

Human cartilage tissue can be categorized into three types: hyaline cartilage, elastic and fibrocartilage. Each type of possesses unique properties functions, which presents a significant challenge for the regeneration repair damaged tissue. Bionics is discipline in humans study imitate nature. A bionic strategy based on comprehensive knowledge anatomy histology human expected to contribute fundamental core elements repair. Moreover, as novel tissue-engineered technology, 3D bioprinting has distinctive advantage rapid precise construction targeted models. Thus, by selecting suitable materials, cells cytokines, leveraging advanced printing technology concepts, it becomes possible simultaneously realize multiple beneficial achieve improved This article provides an overview key involved combination strategies, with particular focus recent advances mimicking different types

Язык: Английский

Процитировано

22