ACS Nano,
Год журнала:
2024,
Номер
19(1), С. 781 - 802
Опубликована: Дек. 21, 2024
Neutrophil
membrane
vesicles
(NMVs)
have
been
successfully
applied
to
control
the
inflammatory
cascade
after
spinal
cord
injury
(SCI)
by
acting
as
an
factor
decoy
front-load
overall
inflammation
regulatory
window;
however,
mechanisms
which
NMVs
regulate
macrophage
phenotypic
shifts
well
their
outcomes
rarely
reported.
In
this
study,
we
demonstrated
"efferocytosis-like"
effect
of
endocytosed
macrophages,
supplementing
TCA
cycle
intermediate
metabolite
α-KG
promoting
glutamine
metabolism,
in
turn
facilitates
oxidative
phosphorylation
and
inhibits
NF-κB
signaling
pathway
reprogram
macrophages
pro-regenerative
phenotype.
Based
on
these
findings,
a
"Trojan
horse"
composite
fiber
scaffold
was
constructed;
comprised
carboxylated
poly-l-lactic
acid
shell
encapsulated
with
core
loaded
brain-derived
neurotrophic
spatiotemporally
modulate
microenvironment
39.23%
sustainably
promote
nerve
regeneration
85.67%.
vivo
experiments
further
confirmed
NMV-coated
scaffolds
regulation
early
innate
immune
continuous
promotion
regeneration.
This
study
not
only
unravels
mechanism
neutrophil
membrane–macrophage
interactions
but
also
provides
strategy
for
coordinating
reprogramming
following
SCI.
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(38)
Опубликована: Апрель 21, 2024
Abstract
Persistent
oxidative
stress
and
bacterial
infection
are
significant
challenges
that
impede
diabetic
wound
healing.
By
combining
diagnosis
treatment,
pH
variation
on
the
tissue
can
be
monitored
in
real
time,
precise
treatment
carried
out
promptly
to
promote
In
this
study,
a
lipoic
acid‐modified
chitosa
(LAMC)
hydrogel
is
constructed
via
an
amidation
reaction,
ceria
oxide‐molybdenum
disulfide
nanoparticles
with
polydopamine
layer
(C@M@P),
along
carbon
quantum
dots
(CDs)
synthesized
by
hydrothermal
method,
loaded
into
hydrogel,
thus
developing
diagnostic
therapeutic
(LAMC/CD‐C@M@P).
incorporating
CDs,
exhibits
high
sensitivity
reversibility
under
ultraviolet
light.
Furthermore,
images
of
hydrogels
collected
using
smartphones
converted
signals,
providing
means
for
early
detection
infection.
Notably,
LAMC/CD‐C@M@P
excellent
photothermal
antibacterial
capability
against
Staphylococcus
aureus
Escherichia
coli
remarkable
antioxidant
anti‐inflammatory
abilities
alleviate
reactive
oxygen
species
relieve
inflammation
response.
summary,
multifunctional
offers
great
potential
as
innovative
dressing
platform,
representing
advancement
chronic
management.
Journal of Nanobiotechnology,
Год журнала:
2023,
Номер
21(1)
Опубликована: Июль 24, 2023
Abstract
Functional
hydrogels
show
potential
application
in
repairing
spinal
cord
injury
(SCI)
due
to
their
unique
chemical,
physical,
and
biological
properties
functions.
In
this
comprehensive
review,
we
present
recent
advance
the
material
design,
functional
regulation,
SCI
repair
applications
of
bioactive
hydrogels.
Different
from
previously
released
reviews
on
three-dimensional
scaffolds
for
repair,
work
focuses
strategies
design
biologically
regulation
hydrogels,
specifically
aiming
how
these
significant
efforts
can
promoting
performance
SCI.
We
demonstrate
various
methods
techniques
fabrication
with
components
such
as
DNA,
proteins,
peptides,
biomass
polysaccharides,
biopolymers
obtain
including
cell
biocompatibility,
self-healing,
anti-bacterial
activity,
injectability,
bio-adhesion,
bio-degradation,
other
multi-functions
The
drugs/growth
factors,
polymers,
nanoparticles,
one-dimensional
materials,
two-dimensional
materials
highly
effective
treating
are
introduced
discussed
detail.
This
shows
new
viewpoints
ideas
synthesis
state-of-the-art
knowledges
science
nanotechnology,
will
bridge
connection
biomedicine,
further
inspire
clinical
biomedical
fields.
Advanced Healthcare Materials,
Год журнала:
2023,
Номер
13(3)
Опубликована: Ноя. 10, 2023
Abstract
Stem
cell
tissue
engineering
is
a
potential
treatment
for
osteoarthritis.
However,
the
number
of
stem
cells
that
can
be
delivered,
loss
during
injection,
and
migration
ability
limit
applications
traditional
engineering.
Herein,
kartogenin
(KGN)‐loaded
poly(lactic
‐co‐
glycolic
acid)
(PLGA)
porous
microspheres
first
engineered
via
emulsification,
then
anchored
with
chitosan
through
amidation
reaction
to
develop
new
microsphere
(PLGA‐CS@KGN)
as
expansion
vector.
Following
3D
co‐culture
PLGA‐CS@KGN
carrier
mesenchymal
(MSCs),
delivery
system
injected
into
capsule
cavity
in
situ.
In
vivo
vitro
experiments
show
PLGA‐CS
have
high
cell‐carrying
capacity
up
1
×
10
4
mm
−3
provide
effective
protection
MSCs
promote
their
controlled
release
osteoarthritis
microenvironment.
Simultaneously,
KGN
loaded
inside
effectively
cooperated
induce
differentiate
chondrocytes.
Overall,
these
findings
indicate
held
cell‐loading
ability,
adapt
cells,
express
markers
associated
cartilage
repair.
Thus,
used
enhancing
therapy
treatment.
Advanced Healthcare Materials,
Год журнала:
2023,
Номер
12(12)
Опубликована: Янв. 18, 2023
The
inflammatory
cascade
after
spinal
cord
injury
(SCI)
causes
necrotizing
apoptosis
of
local
stem
cells,
which
limits
nerve
regeneration.
Therefore,
coordinating
the
immune
response
and
neural
cell
(NSC)
functions
is
key
to
promoting
recovery
central
nervous
system
function.
In
this
study,
a
hydrogel
"perfusion"
electrospinning
technology
are
integrated,
"concrete"
composite
support
for
repair
injuries
built.
hydrogel's
hydrophilic
properties
activate
macrophage
integrin
receptors
mediate
polarization
into
anti-inflammatory
subtypes
cause
10%
increase
in
polarized
M2
macrophages,
thus
reprogramming
SCI
microenvironment.
Programmed
stromal
cell-derived
factor-1α
brain-derived
neurotrophic
factor
released
from
recruitment
neuronal
differentiation
NSCs
by
approximately
four-
twofold,
respectively.
fiber
regulates
microenvironment,
recruits
endogenous
NSCs,
promotes
blood
vessel
germination
maturation,
improves
function
rat
model.
conclusion,
engineering
response.
It
regeneration
through
programmed
cytokine-delivery
system,
further
supplements
mechanism
regulated
inherent
biomaterial.
new
may
serve
as
treatment
approach
SCI.
Regenerative Biomaterials,
Год журнала:
2024,
Номер
11
Опубликована: Янв. 1, 2024
Abstract
Decellularized
extracellular
matrix
hydrogel,
especially
that
derived
from
spinal
cord
(DSCM
hydrogel),
has
been
actively
considered
as
a
functional
biomaterial
for
remodeling
the
of
native
tissue,
due
to
its
unique
characteristics
in
constructing
pro-regenerative
microenvironment
neural
stem
cells
(NSCs).
Furthermore,
DSCM
hydrogel
can
provide
multiple
binding
domains
growth
factors
and
drugs.
Therefore,
both
exogenous
neurotrophic
anti-inflammatory
drugs
are
highly
desired
be
incorporated
into
which
may
synergistically
modulate
complex
at
lesion
site
after
injury
(SCI).
Herein,
neurotrophin-3
(NT-3)
curcumin
(Cur)
were
integrated
SCI
therapy.
Due
different
affinities
NT-3
underwent
controlled
release
manner,
while
released
explosively
within
first
24
h,
followed
by
rather
sustained
but
slower
release.
The
integration
significantly
enhanced
NSCs
proliferation
their
neuronal
differentiation.
Meanwhile,
promoted
macrophages
polarization
subtypes,
further
facilitated
differentiation
neurons.
situ
injected
+
NT3
Cur
exerted
superior
capability
alleviating
inflammatory
responses
rat
contused
cord.
Compared
alone,
more
recruitment
site.
These
outcomes
favored
recovery,
evidenced
improved
hind
limb
movement.
Overall,
bioactive
serve
multifunctional
carrier
cooperatively
drugs,
benefits
regulation
nerve
regeneration
SCI.
Pharmaceutics,
Год журнала:
2024,
Номер
16(1), С. 134 - 134
Опубликована: Янв. 19, 2024
The
brain
consists
of
an
interconnected
network
neurons
tightly
packed
in
the
extracellular
matrix
(ECM)
to
form
complex
and
heterogeneous
composite
tissue.
According
recent
biomimicry
approaches
that
consider
biological
features
as
active
components
biomaterials,
designing
a
highly
reproducible
microenvironment
for
cells
can
represent
key
tool
tissue
repair
regeneration.
Indeed,
this
is
crucial
support
cell
growth,
mitigate
inflammation
phenomena
provide
adequate
structural
properties
needed
damaged
tissue,
corroborating
activity
vascular
ultimately
functionality
neurons.
In
context,
electro-fluid
dynamic
techniques
(EFDTs),
i.e.,
electrospinning,
electrospraying
related
techniques,
offer
opportunity
engineer
wide
variety
substrates
by
integrating
fibers,
particles,
hydrogels
at
different
scales—from
several
hundred
microns
down
tens
nanometers—for
generation
countless
patterns
physical
biochemical
cues
suitable
influencing
vitro
response
coexistent
populations
mediated
surrounding
microenvironment.
review,
overview
technological
approaches—based
on
EFDTs—for
engineering
fibrous
and/or
particle-loaded
will
be
proposed.
second
section
review
primarily
focus
describing
current
future
use
composites
applications,
ranging
from
therapeutic
diagnostic/theranostic
regeneration,
with
ultimate
goal
providing
insightful
information
guide
research
efforts
toward
development
more
efficient
reliable
solutions.
