Materials & Design,
Год журнала:
2023,
Номер
235, С. 112441 - 112441
Опубликована: Окт. 30, 2023
Rational
design
of
organelle
targeted
therapeutic
platform
in
where
the
drug
can
be
to
destroy
tumor
cells
as
much
possible,
is
one
effective
ways
improve
outcomes
and
minimize
cytotoxicity
normal
cells.
Here,
photosensitizer
TSPy-B
with
aggregation-induced
emission
(AIE)
feature,
biotin
receptor
mitochondria
dual-targeted
capability,
high
reactive
oxygen
species
(ROS)
generation
efficiency
was
designed
for
fluorescence
imaging
guided
photodynamic
therapy
(PDT)
by
linking
group
D-π-A
type
AIE
dyes
via
ester
bonds.
Notably,
cell
experiments
showed
that
could
selectively
target
overexpression
further
exhibit
targeting
ability.
The
vitro
anticancer
mechanism
confirmed
after
internalization,
localized
mitochondrial
site
produced
ROS
resulting
dysfunction
death.
simultaneously
produce
1O2
highly
toxic
free
radicals
which
not
depended
on
level
solid
tumor.
killing
effect
also
hypoxic
culture
model.
In
vivo
demonstrated
effectively
inhibit
growth
had
good
biological
safety.
This
work
provides
a
choice
development
photosensitizers
clinical
PDT.
Cancer Letters,
Год журнала:
2023,
Номер
558, С. 216093 - 216093
Опубликована: Фев. 22, 2023
Exosomes
are
small
phospholipid
bilayer
vesicles
that
naturally
produced
by
all
living
cells,
both
prokaryotes
and
eukaryotes.
The
exosomes
due
to
their
unique
size,
reduced
immunogenicity,
ability
mimic
synthetic
liposomes
in
carrying
various
anticancer
drugs
have
been
tested
as
drug
delivery
vehicles
for
cancer
treatment.
An
added
advantage
of
developing
a
carrier
is
the
ease
manipulating
intraluminal
content
surface
modification
achieve
tumor-targeted
delivery.
In
past
ten-years,
there
has
an
exponential
increase
number
exosome-related
studies
cancer.
Preclinical
demonstrate
exosomes-mediated
chemotherapeutics,
biologicals
natural
products
produce
potent
activity
both,
vitro
vivo.
contrast,
exosome-based
clinical
trials
few
challenges
manufacturing
scalability
related
large-scale
production
storage
stability.
Herein,
we
discuss
recent
advances
treatment
preclinical
conclude
with
be
overcome
translating
larger
therapies
into
clinic.
Journal of Nanobiotechnology,
Год журнала:
2023,
Номер
21(1)
Опубликована: Март 29, 2023
Abstract
Background
The
past
few
years
have
witnessed
a
significant
increase
in
research
related
to
plant-derived
extracellular
vesicles
(PDEVs)
biological
and
medical
applications.
Using
biochemical
technologies,
multiple
independent
groups
demonstrated
the
important
roles
of
PDEVs
as
potential
mediators
involved
cell-cell
communication
exchange
bio-information
between
species.
Recently,
several
contents
been
well
identified
PDEVs,
including
nucleic
acids,
proteins,
lipids,
other
active
substances.
These
cargoes
carried
by
could
be
transferred
into
recipient
cells
remarkably
influence
their
behaviors
associated
with
human
diseases,
such
cancers
inflammatory
diseases.
Main
body
abstract
This
review
summarizes
latest
updates
regarding
focuses
on
its
role
nanomedicine
applications,
drug
delivery
strategies
develop
diagnostic
therapeutic
agents
for
clinical
management
especially
like
cancers.
Conclusion
Considering
unique
advantages,
high
stability,
intrinsic
bioactivity
easy
absorption,
further
elaboration
molecular
mechanisms
factors
driving
function
will
provide
new
horizons
treatment
disease.
Abstract
In
recent
decades,
research
on
Extracellular
Vesicles
(EVs)
has
gained
prominence
in
the
life
sciences
due
to
their
critical
roles
both
health
and
disease
states,
offering
promising
applications
diagnosis,
drug
delivery,
therapy.
However,
inherent
heterogeneity
complex
origins
pose
significant
challenges
preparation,
analysis,
subsequent
clinical
application.
This
review
is
structured
provide
an
overview
of
biogenesis,
composition,
various
sources
EVs,
thereby
laying
groundwork
for
a
detailed
discussion
contemporary
techniques
preparation
analysis.
Particular
focus
given
state‐of‐the‐art
technologies
that
employ
microfluidic
non‐microfluidic
platforms
EV
processing.
Furthermore,
this
discourse
extends
into
innovative
approaches
incorporate
artificial
intelligence
cutting‐edge
electrochemical
sensors,
with
particular
emphasis
single
proposes
current
outlines
prospective
avenues
future
research.
The
objective
motivate
researchers
innovate
expand
methods
analysis
fully
unlocking
biomedical
potential.
Materials Today Bio,
Год журнала:
2025,
Номер
30, С. 101443 - 101443
Опубликована: Янв. 5, 2025
Glioblastoma
(GBM)
is
the
most
prevalent
primary
malignant
brain
tumor,
characterized
by
a
high
mortality
rate
and
poor
prognosis.
The
blood-brain
barrier
(BBB)
blood-tumor
(BTB)
present
significant
obstacles
to
efficacy
of
tumor-targeted
pharmacotherapy,
thereby
impeding
therapeutic
potential
numerous
candidate
drugs.
Targeting
delivery
adequate
doses
drug
across
BBB
treat
GBM
has
become
prominent
research
area
in
recent
years.
This
emphasis
driven
exploration
evaluation
diverse
technologies
for
with
some
already
undergoing
clinical
trials.
review
provides
thorough
overview
advancements
challenges
targeted
treatment.
It
specifically
emphasizes
systemic
administration
strategies
assess
their
limitations
Furthermore,
this
highlights
promising
future
directions
development
intelligent
systems
aimed
at
overcoming
current
enhancing
against
GBM.
These
not
only
support
foundational
on
but
also
offer
methodological
approaches
applications.
Extracellular
vesicles
(EVs)
are
critical
mediators
of
intercellular
communication,
carrying
bioactive
cargo
and
displaying
diverse
surface
components
that
reflect
their
cellular
origins
functions.
The
EV
surface,
composed
proteins,
lipids,
glycocalyx
elements,
plays
a
pivotal
role
in
targeting
recipient
cells,
mediating
biological
interactions,
enabling
selective
delivery.
This
review
comprehensively
examined
the
molecular
architecture
surfaces,
linking
biogenesis
to
functional
diversity,
highlights
therapeutic
diagnostic
potential
diseases
such
as
cancer
cardiovascular
disorders.
Additionally,
we
explore
emerging
applications
EVs,
including
machine-learning-assisted
analysis,
chemical
integration,
cross-system
combinations.
also
discusses
some
key
challenges
clinical
translation
EV-related
technologies.
Journal of Advanced Research,
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 1, 2024
MicroRNAs
(miRNAs)
involve
in
destabilising
messenger
RNA
or
repressing
translation
of
target
molecules.
Ginger-derived
exosome-like
nanoparticles
(GELNs)
play
a
crucial
role
modulating
intestinal
inflammation.
Moreover,
GELNs
contain
highly
heterogeneous
miRNA.
However,
the
miRNAs
derived
from
immunomodulation
remains
unclear.
This
study
aimed
to
elucidate
molecular
basis
unique
biological
effects
mediated
by
miRNA
on
macrophages.
were
isolated
using
combination
commercial
exosome
isolation
kits
and
differential
centrifugation
method,
lipid
composition
was
determined
liquid
chromatography-mass
spectrometry.
Subsequently,
PKH26
labelled
taken
up
Furthermore,
modulation
inflammatory
immune
responses
osa-miR164d
assessed
through
RNA-seq,
RT-qPCR,
online
databases,
dual
luciferase
reporter
assays
explore
underlying
mechanisms
osa-miR164d.
Biomimetic
exosomes
loaded
with
prepared
microfluidic
mixing
device
systematically
characterized.
The
therapeutic
relieving
colitis
evaluated.
We
report
for
first
time
that
GELNs-derived
is
regulatory
factor
reprogramming
macrophage
polarization,
thereby
inhibiting
response.
Mechanistically,
directly
targets
3'-UTRs
TAB1,
which
regulates
polarization
downregulation
NF-κB
expression.
In
addition,
have
designed
biomimetic
mimicking
deliver
(osa-miR164d-MGELNs).
Notably,
osa-miR164d-MGELNs
can
efficiently
reprogram
macrophages
alleviate
colitis-related
symptoms.
Our
findings
enhance
systematic
understanding
how
mediates
cross-kingdom
communication
provide
an
original
engineering
paradigm
transfer
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 2591 - 2610
Опубликована: Март 1, 2024
Extracellular
vesicles
can
transmit
intercellular
information
and
transport
biomolecules
to
recipient
cells
during
various
pathophysiological
processes
in
the
organism.
Animal
cell
exosomes
have
been
identified
as
potential
nanodrugs
delivery
vehicles,
yet
they
some
shortcomings
such
high
immunogenicity,
cytotoxicity,
complicated
preparation
procedures.
In
addition
exosomes,
plant-derived
extracellular
(PDVs),
which
carry
a
variety
of
active
substances,
are
another
promising
nano-transport
vehicles
emerging
recent
years
due
their
stable
physicochemical
properties,
wide
source,
low
cost.
This
work
briefly
introduces
collection
characterization
PDVs,
then
focuses
on
application
PDVs
natural
or
engineered
drug
carriers
biomedicine,
finally
discusses
development
challenges
future
applications.
Foods,
Год журнала:
2024,
Номер
13(5), С. 712 - 712
Опубликована: Фев. 26, 2024
Plant-derived
exosome-like
nanovesicles
(PELNs)
are
bilayer
membrane-enclosed
secreted
by
plant
cells,
serving
as
carriers
of
various
substances
such
proteins,
RNA,
and
metabolites.
The
mounting
evidence
suggests
that
PELN
plays
a
crucial
role
in
transmembrane
signaling,
nutrient
transportation,
apoptosis,
regulation
gut
microbiota
composition.
This
makes
it
promising
"dark
nutrient"
for
plants
to
modulate
human
physiology
pathogenesis.
A
comprehensive
understanding
formation,
uptake,
functional
mechanisms
can
offer
novel
insights
into
nutrition
properties,
thereby
facilitating
the
precise
development
plant-based
foods
drugs.
article
provides
summary
extraction
characterization,
well
absorption
delivery
processes.
Furthermore,
focuses
on
latest
discoveries
underlying
physiological
PELN's
functions
while
exploring
future
research
directions.
