Sustainable Food Technology, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Fruit exosomes are the source of natural cancer therapeutic tools.
Язык: Английский
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Фев. 7, 2025
Despite the approval of several artificial nanotherapeutics for treatment triple-negative breast cancer (TNBC), significant challenges, including unsatisfactory therapeutic outcomes, severe side effects, and high cost large-scale production, still restrict their long-term application. In contrast, plant-derived extracellular vesicles (PEVs) exhibit promising potential in therapy due to negligible systemic toxicity, bioavailability cost- effectiveness. this study, we developed an alternative strategy inhibit TNBC via Platycodon grandiflorum (PG)-derived (PGEVs). The PGEVs were isolated by ultracentrifugation sucrose gradient centrifugation method contained adequate functional components such as proteins, lipids, RNAs active molecules. exhibited remarkable stability, tolerating acidic digestion undergoing minimal changes simulated gastrointestinal fluid. They efficiently taken up tumor cells induced increased production reactive oxygen species (ROS), leading cell proliferation inhibition apoptosis, particularly line 4T1. Additionally, facilitated polarization tumor-associated macrophages (TAMs) toward M1 phenotype secretion pro-inflammatory cytokines. Further vivo investigations revealed that accumulated 4T1 tumors exerted effects through boosting anti-tumor immune responses modulating gut microbiota whether administered orally or intravenously (i.v.). conclusion, these findings highlight a natural, biocompatible efficient nanotherapeutic candidate treating TNBC.
Язык: Английский
Процитировано
2
Frontiers in Nutrition, Год журнала: 2025, Номер 12
Опубликована: Март 5, 2025
Plant-derived exosome-like nanoparticles (PDENs), emerging as novel bioactive agents, exhibit significant potential in food science and nutritional health. These nanoparticles, enriched with plant-specific biomolecules such proteins, lipids, nucleic acids, secondary metabolites, demonstrate unique cross-species regulatory capabilities, enabling interactions mammalian cells gut microbiota. PDENs enhance nutrient bioavailability by protecting sensitive compounds during digestion, modulate metabolic pathways through miRNA-mediated gene regulation, anti-inflammatory antioxidant properties. For instance, grape-derived reduce plasma triglycerides high-fat diets, while ginger-derived alleviate colitis downregulating pro-inflammatory cytokines. Additionally, serve natural drug carriers, applications delivering therapeutic agents like doxorubicin paclitaxel. Despite these advancements, challenges remain standardizing extraction methods (ultracentrifugation, immunoaffinity), ensuring stability processing storage, evaluating long-term safety. Current research highlights the need for optimizing lyophilization techniques understanding between matrices. Furthermore, show promise functional development—such fortified beverages probiotic formulations—their clinical translation requires rigorous pharmacokinetic studies clarity. This review synthesizes existing knowledge on PDENs’ composition, biological activities, applications, identifying gaps scalability, stability, safety assessments. Future directions emphasize interdisciplinary collaboration to harness combating disorders, enhancing functionality, advancing personalized nutrition strategies.
Язык: Английский
Процитировано
1
Drug Delivery and Translational Research, Год журнала: 2024, Номер unknown
Опубликована: Май 17, 2024
Язык: Английский
Процитировано
5
Bioactive Materials, Год журнала: 2024, Номер 40, С. 484 - 502
Опубликована: Июнь 28, 2024
The rate of retear after surgical repair remains high. Mesenchymal stem cells (MSCs) have been extensively employed in regenerative medicine for several decades. However, safety and ethical concerns constrain their clinical application. Tendon Stem/Progenitor Cells (TSPCs)-derived exosomes emerged as promising cell-free therapeutic agents. Therefore, urgent studies are needed to investigate whether TSPC-Exos could enhance tendon-bone healing elucidate the underlying mechanisms. In this study, were found promote proliferation, migration, expression fibrogenesis markers BMSCs. Furthermore, demonstrated an ability suppress polarization M1 macrophages while promoting M2 macrophage polarization. a rat model rotator cuff repair, modulated inflammation improved histological structure interface, biomechanical properties repaired tendon, function joint. Mechanistically, exhibited high miR-21a-5p, which regulated PDCD4. PDCD4/AKT/mTOR axis was implicated effects on This study introduces novel approach utilizing therapy strategy therapies, potentially benefiting patients with tear future.
Язык: Английский
Процитировано
4
Advanced Biology, Год журнала: 2025, Номер unknown
Опубликована: Апрель 8, 2025
Abstract Extracellular vesicles (EVs) are nanoscale luminal vesicles, which play an important role in intercellular communication through surface signaling and molecular cargo delivery (proteins, lipids, nucleic acids, etc.). Recently, plant‐derived extracellular (PDVs) containing multiple biological activities have received increasing attention due to their better biocompatibility lower cytotoxicity healthy tissues. In the biomedical field, PDVs employed as vehicles, enabling diverse functionalities engineering modification techniques. Nonetheless, there certain issues with study of PDVs, such lack standardization identification isolation criteria. This review provides a quick overview biogenesis, physicochemical properties, techniques, applications current studies, while critically analyzing challenges opportunities. paper is expected provide some theoretical guidance for development further applications.
Язык: Английский
Процитировано
0
Advanced Science, Год журнала: 2024, Номер unknown
Опубликована: Окт. 18, 2024
Abstract Electrodynamic therapy (EDT) is a promising alternative approach for antibacterial therapy, as reactive oxygen species (ROS) are produced efficiently in response to an electric field without relying on endogenous H 2 O and . However, the inherent toxicity of metallic catalysts numerous bacterial toxins during therapeutic process still hinder its development. Herein, biomimetic metal–organic (MOF@EV) nanosponges composed ginger‐derived extracellular vesicles (EVs), electrodynamic frameworks (MOFs) developed eradication infections absorption toxins. The prolonged circulation time MOF@EV vivo facilitates their accumulation at infection sites. More interestingly, can behave effectively prevent host cells from binding toxins, thereby reducing damage cells. Subsequently, discovered work electro‐sensitizers, which confirmed through both theoretical calculation experimental verification. As result, ROS continuously under achieve effective EDT‐mediated eradication. Meanwhile, treatment visualized mice infected with luciferase‐expressing Staphylococcus aureus ( S. ), excellent biofilm capacity detoxification efficiency demonstrated subcutaneous abscess model. This provides strategy infections.
Язык: Английский
Процитировано
3
Processes, Год журнала: 2024, Номер 12(12), С. 2938 - 2938
Опубликована: Дек. 23, 2024
Plant-derived extracellular vesicles (PDEVs) are lipid bilayer nanoparticles, naturally produced by plant cells, with sizes ranging from 50 to 500 nm. Recent studies have highlighted their great potential in the biotechnological and medical fields, due natural origin, high biocompatibility intrinsic therapeutic properties. PDEVs contain a complex biological cargo of proteins, lipids, nucleic acids secondary metabolites, including antioxidants anti-inflammatory molecules, making them ideal for biomedical applications such as drug delivery. These play key role intercellular communication gene regulation, proving be particularly promising personalized medicine. ability improve stability bioavailability, optimizing targeted release minimizing side effects. Despite some challenges, compositional variability need standardized protocols, at gunsight innovative research aimed improving loading capacity specificity. This review aims provide comprehensive overview PDEVs, exploring structure, isolation methods, functional characteristics, applications, highlighting advantages over synthetic nanoparticles animal-derived vesicles, leading an sustainable solution development new approaches.
Язык: Английский
Процитировано
3
Toxics, Год журнала: 2024, Номер 12(9), С. 653 - 653
Опубликована: Сен. 5, 2024
Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants that pose significant environmental and health risks. These compounds originate from both natural phenomena, such as volcanic activity wildfires, anthropogenic sources, including vehicular emissions, industrial processes, fossil fuel combustion. Their classification carcinogenic, mutagenic, teratogenic substances link them to various cancers disorders. PAHs categorized into low-molecular-weight (LMW) high-molecular-weight (HMW) groups, with HMW exhibiting greater resistance degradation a tendency accumulate in sediments biological tissues. Soil serves primary reservoir for PAHs, particularly areas of high creating substantial risks through ingestion, dermal contact, inhalation. Coastal aquatic ecosystems especially vulnerable due concentrated human activities, PAH persistence disrupting microbial communities, inhibiting plant growth, altering ecosystem functions, potentially leading biodiversity loss. In plants, contamination manifests form abiotic stress, inducing oxidative cellular damage, growth inhibition. Plants respond by activating antioxidant defenses stress-related pathways. A notable aspect defense mechanisms involves plant-derived extracellular vesicles (PDEVs), which membrane-bound nanoparticles released cells. PDEVs play crucial role enhancing facilitating intercellular communication coordinating responses. The interaction between PDEVs, while not fully elucidated, suggests complex interplay mechanisms. may contribute detoxification pollutant sequestration or delivering enzymes capable degradation. Studying provides valuable insights stress resilience offers potential new strategies mitigating PAH-induced plants ecosystems.
Язык: Английский
Процитировано
2
Horticulturae, Год журнала: 2024, Номер 10(5), С. 477 - 477
Опубликована: Май 7, 2024
Horticultural plant material offers several advantages for isolating exosomes and other natural plant-derived exosome-like nanoparticles (PDENs) due to the accessibility affordability of widespread applications. This study aims explore impacts tomato genotype (‘Admiro’, ‘Roma’, ‘Brooklyn’, ‘Marmande’ ‘Betalux’) main cultivation parameters in controlled environment agriculture on yield properties their PDENs pharmaceutical cosmeceutical The PDEN yield, size distribution, antioxidative young seedlings were evaluated. ‘Betalux’ was distinguished by a remarkably higher nanoparticle concentration uniform distribution selected further experiments. impact temperature (18, 22, 26 °C), nitrogen nutrition (0, 250, 500 mg L−1), lighting photosynthetic photon flux density (PPFD; 150, 450 µmol m−2 s−1) investigated. Optimal conditions consisting 22 °C, 250 L−1 nutrition, s−1 PPFD used as reference. temperature, intensity resulted highest most particle preparations keratinocyte metabolic activity. Deviation from optimal reduced biomass protein yield.
Язык: Английский
Процитировано
1
Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)
Опубликована: Ноя. 25, 2024
Extracellular vesicles (EVs) are phospholipid bilayer-enclosed biological particles that secreted by almost all living cells including animals, plants, and microorganisms. Chinese herbal medicines (CHM) have a long history of using plant-based remedies to treat prevent human diseases. medicine-derived extracellular vesicle (CHMEV) generic term refers nanoscale membrane structures isolated from medicinal plants such as ginseng, ginger, Panax notoginseng. In recent years, CHMEVs garnered substantial attention novel class functional components due their high bioavailability, safety, easy accessibility, diverse therapeutic effects, indicating great potential for development new dosage form CHM. Research on in traditional medicine (TCM) has become prominent area interest, opening avenues further exploration into the effects mechanisms Nonetheless, an emerging field, there is much unknown about these vesicles, current research remains inconsistent. The review comprehensively summarizes biogenesis, isolation methods, physical, biochemical characterizations CHMEVs. Additionally, we highlight biomedical applications agents drug delivery carriers, anti-inflammatory, anticancer, regenerative, antiaging activities. Finally, propose challenges future perspectives. By summarizing existing literature, aim offer valuable clues inspiration CHMEV research, thereby facilitating standardization treatment diseases discovery.
Язык: Английский
Процитировано
1