Bioactive Materials,
Год журнала:
2024,
Номер
39, С. 521 - 543
Опубликована: Май 30, 2024
Spinal
cord
injury
(SCI)
often
results
in
irreversible
loss
of
sensory
and
motor
functions,
most
SCIs
are
incurable
with
current
medical
practice.
One
the
hardest
challenges
treating
SCI
is
development
a
dysfunctional
pathological
microenvironment,
which
mainly
comprises
excessive
inflammation,
deposition
inhibitory
molecules,
neurotrophic
factor
deprivation,
glial
scar
formation,
imbalance
vascular
function.
To
overcome
this
challenge,
implantation
functional
biomaterials
at
site
has
been
regarded
as
potential
treatment
for
modulating
microenvironment
to
support
axon
regeneration,
remyelination
site,
recovery
after
SCI.
This
review
summarizes
characteristics
recent
advances
well
technologies
used
modulate
inflammatory
regulate
reshape
revascularization
microenvironment.
Moreover,
technological
limitations,
challenges,
future
prospects
promote
efficient
repair
also
discussed.
will
aid
further
understanding
Bioactive Materials,
Год журнала:
2024,
Номер
35, С. 242 - 258
Опубликована: Фев. 3, 2024
Induced
pluripotent
stem
cells
(iPSCs)
can
be
personalized
and
differentiated
into
neural
(NSCs),
thereby
effectively
providing
a
source
of
transplanted
for
spinal
cord
injury
(SCI).
To
further
improve
the
repair
efficiency
SCI,
we
designed
functional
network
tissue
based
on
TrkC-modified
iPSC-derived
NSCs
CBD-NT3-modified
linear-ordered
collagen
scaffold
(LOCS).
We
confirmed
that
transplantation
this
regenerated
neurons
synapses,
improved
microenvironment
injured
area,
enhanced
remodeling
extracellular
matrix,
promoted
recovery
hind
limbs
in
rat
SCI
model
with
complete
transection.
RNA
sequencing
metabolomic
analyses
also
effect
from
multiple
perspectives
revealed
its
potential
mechanism
treating
SCI.
Together,
constructed
using
human
iPSCs-derived
as
seed
interaction
receptors
ligands
first
time.
This
therapeutic
thus
confirming
feasibility
LOCS
valuable
direction
research.
Military Medical Research,
Год журнала:
2024,
Номер
11(1)
Опубликована: Окт. 21, 2024
Abstract
Severe
tissue
defects
present
formidable
challenges
to
human
health,
persisting
as
major
contributors
mortality
rates.
The
complex
pathological
microenvironment,
particularly
the
disrupted
immune
landscape
within
these
defects,
poses
substantial
hurdles
existing
regeneration
strategies.
However,
emergence
of
nanobiotechnology
has
opened
a
new
direction
in
immunomodulatory
nanomedicine,
providing
encouraging
prospects
for
and
restoration.
This
review
aims
gather
recent
advances
nanomedicine
foster
regeneration.
We
begin
by
elucidating
distinctive
features
local
microenvironment
defective
tissues
its
crucial
role
Subsequently,
we
explore
design
functional
properties
nanosystems.
Finally,
address
clinical
translation
development,
aiming
propose
potent
approach
enhance
through
synergistic
modulation
integration.
Spinal
cord
injury
(SCI)
remains
a
formidable
challenge
in
biomedical
research,
as
the
silencing
of
intrinsic
regenerative
signals
most
spinal
neurons
results
an
inability
to
reestablish
neural
circuits.
In
this
study,
we
found
that
with
low
axonal
regeneration
after
SCI
showed
decreased
extracellular
signal-regulated
kinase
(ERK)
phosphorylation
levels.
However,
expression
dual
specificity
phosphatase
26
(DUSP26)─which
negatively
regulates
ERK
phosphorylation─was
reduced
considerably
undergoing
spontaneous
regeneration.
Therefore,
developed
system
named
F10@MS@UV-HG
integrated
DUSP26-specific
inhibitor
into
reactive
oxygen
species-responsive
nanoparticles
and
embedded
them
photosensitive
hydrogels.
This
effectively
downregulated
DUSP26
primary
enhanced
phosphorylation,
ultimately
promoting
outgrowth.
When
transplanted
mouse
model,
achieved
sustained
drug
release,
specifically
targeting
DUSP26/ERK/ELK1
pathway
facilitating
short-term
Additionally,
long-term
repair
effects─including
improved
myelination
motor
function─were
evident
mice
F10@MS@UV-HG.
The
suggested
activating
signaling
by
modulating
could
promote
functional
recovery.
Thus,
exhibits
enormous
potential
therapeutic
approach
for
patients
SCI.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Янв. 29, 2025
Neuromuscular
diseases
usually
manifest
as
abnormalities
involving
motor
neurons,
neuromuscular
junctions,
and
skeletal
muscle
(SkM)
in
postnatal
stage.
Present
vitro
models
of
interactions
require
a
long
time
lack
neuroglia
involvement.
Our
study
aimed
to
construct
rodent
bioengineered
spinal
cord
neural
network-skeletal
(NN-SkM)
assembloids
elucidate
the
between
stem
cells
(SC-NSCs)
SkM
their
biological
effects
on
development
maturation
circuits.
After
coculture
with
cells,
SC-NSCs
developed
into
networks
(NNs)
exhibited
high
proportion
glutamatergic
cholinergic
low
gamma-aminobutyric
acidergic
increased
expression
synaptic
markers.
In
NN-SkM
assembloids,
acetylcholine
receptors
were
upregulated,
generating
junction-like
structures
NNs.
The
amplitude
frequency
cell
contraction
by
optogenetic
glutamate
stimulation
blocked
tetrodotoxin
dizocilpine,
respectively,
confirming
existence
multisynaptic
process
involves
secretion
neurotrophin-3
insulin
growth
factor-1
which
activate
related
ERK-MAPK
PI3K-AKT
signaling
pathways
Inhibition
significantly
reduces
neuronal
differentiation
while
also
decreasing
receptor
formation
cells.
brief,
simulate
composition
NNs
respond
regulatory
signals,
providing
an
model
for
studying
Molecular Medicine,
Год журнала:
2025,
Номер
31(1)
Опубликована: Фев. 9, 2025
Abstract
Background
Inflammation
and
proinflammatory
programmed
cell
death,
referred
to
as
pyroptosis,
are
important
causes
of
poor
functional
recovery
after
traumatic
spinal
cord
injury
(TSCI).
Heat
shock
protein
family
A
member
1A
(HSPA1A)
is
a
molecular
chaperone
that
highly
expressed
TSCI
thought
be
neuroprotective.
However,
the
mechanisms
underlying
protective
effects
HSPA1A
unclear.
Methods
The
levels
pyroptosis
inflammation
were
determined
by
enzyme-linked
immunosorbent
assay
(ELISA)
western
blotting
analysis.
role
in
regulating
was
verified
vivo
vitro
experiments.
mechanism
elucidated
bioinformatics
coimmunoprecipitation
analyses.
Results
Pyroptosis
significantly
increased
TSCI.
overexpression
microglia
attenuated
nigericin-
lipopolysaccharide
(LPS)-induced
vitro,
whereas
knockdown
aggravated
inflammation.
In
vivo,
reduced
tissue
damage,
nerve
rats
promoted
recovery.
Mechanistically,
we
identified
interacts
with
dual
specificity
phosphatase
1
(DUSP1)
inhibits
activation
mitogen-activated
kinase
(MAPK)
pathway,
thereby
attenuating
Conclusion
reduces
upregulating
DUSP1
inhibiting
MAPK
pathway
activation.
has
potential
therapeutic
approach
promote
Experimental Neurology,
Год журнала:
2024,
Номер
375, С. 114739 - 114739
Опубликована: Фев. 22, 2024
Spinal
cord
injury
(SCI)
is
a
disorder
of
the
central
nervous
system
resulting
from
various
factors
such
as
trauma,
inflammation,
tumors,
and
other
etiologies.
This
condition
leads
to
impairment
in
motor,
sensory,
autonomic
functions
below
level
injury.
Limitations
current
therapeutic
approaches
prompt
an
investigation
into
angiogenesis
through
persistent
local
expression
proangiogenic
factors.
Here,
we
investigated
whether
overexpression
adeno-associated
virus
(AAV)-mediated
vascular
endothelial
growth
factor
A
(VEGFA)
mouse
SCI
promoted
locomotor
function
recovery,
phosphoinositide
3-kinase
(PI3K)/protein
kinase
B
(Akt)
pathway
was
mechanistically
involved.
Three
weeks
before
SCI,
AAV-VEGFA
injected
at
T10
induce
VEGFA
overexpression.
Neurofunctional,
histological,
biochemical
assessments
were
done
determine
tissue
damage
and/or
recovery
neuromuscular
behavioral
impairments.
Daily
injections
PI3K/Akt
inhibitor
LY294002
made
assess
possible
mechanism.
dramatically
improved
ameliorated
pathological
caused
by
SCI.
Improved
motor-evoked
potentials
hindlimbs
more
spinal
CD31-positive
microvessels
observed
AAV-VEGFA-overexpressing
mice.
reduced
PI3K
Akt
phosphorylation
levels
attenuated
AAV-VEGFA-related
improvements.
In
conclusion,
sustained
AAV-mediated
can
significantly
promote
ameliorate
after
contusion
model
activation
signaling
pathway.
Procedia Computer Science,
Год журнала:
2024,
Номер
231, С. 373 - 378
Опубликована: Янв. 1, 2024
Augmented
and
virtual
reality
technologies
are
actively
making
their
way
into
the
field
of
healthcare,
revolutionizing
traditional
approaches
to
diagnosis,
prevention,
treatment.
This
article
discusses
how
these
concepts
combined
profound
impact
on
emergence
Metahospital.
The
authors
provide
an
overview
this
concept,
highlighting
its
dual
role
in
aiding
healthcare
professionals
empowering
patients.
Central
paradigm
shift
is
technology's
potential
enhance
specialized
training
elevate
precision
efficiency
diagnostics,
all
while
prioritizing
patient
safety.
model's
core
features
expounded
upon,
with
a
keen
emphasis
ethical
considerations
safeguarding
personal
data,
critical
aspect
explored
within
paper.
Furthermore,
elaborates
creation
surgical
simulator
tailored
for
procedures
like
angioplasty
coronary
artery
stenting,
showcasing
tangible
advancements
realm
medical
technology.
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Фев. 7, 2024
Spinal
cord
injury
(SCI)
is
a
devastating
disease
that
causes
extensive
damage
to
oligodendrocytes
and
neurons
leading
demyelination
axonal
degeneration.
In
this
study,
we
co-transplanted
cell
grafts
containing
oligodendrocyte
progenitor
cells
(OPCs)
derived
from
human-induced
pluripotent
stem
(iPSCs)
combined
with
human
umbilical
vein
endothelial
(HUVECs),
which
were
reported
promote
OPCs
survival
migration,
into
rat
contusion
models
functional
recovery
after
SCI.
Theranostics,
Год журнала:
2025,
Номер
15(11), С. 4955 - 4969
Опубликована: Март 31, 2025
Rationale:
Spinal
cord
injury
(SCI)
leads
to
limited
regenerative
capacity
and
severe
energy
deficiency
in
the
microenvironment.
This
study
aimed
develop
a
biomimetic
piezoelectric
hydrogel
system
that
could
recapitulate
native
tissue
microenvironment
while
enabling
wireless
physical
regulation
for
SCI
repair.
Methods:
A
was
fabricated
by
integrating
K0.5Na0.5NbO3
(KNN)
nanoparticles
with
porous
decellularized
spinal
matrix
gel
(pDG).
The
hydrogel's
effects
on
vascular
endothelial
cell
migration,
neural
stem
differentiation,
ATP
synthesis
were
evaluated
vitro.
RNA
sequencing
performed
identify
key
regulatory
pathways.
therapeutic
efficacy
assessed
rat
model
of
hemisection,
examining
motor
function
angiogenesis.
Results:
demonstrated
excellent
biocompatibility
significantly
enhanced
migration.
Under
ultrasonic
stimulation,
promoted
differentiation
into
neurons
more
effectively
than
control
hydrogels.
stimulation
increased
calcium
ion
flux,
activating
Ca2+/Camk2b/PGC-1α
signaling
axis.
In
vivo
studies
showed
implantation
combined
ultrasound
improved
recovery
Conclusion:
presents
an
effective
strategy
repair
through
metabolism
reprogramming
demonstrates
promising
potential
engineering
applications.
