Unidirectional and sustainable release of NGF and BDNF from a chitosan gel-mediated multilayer ordered composite membrane in promoting both spinal cord and sciatic nerve injury repair DOI

Weimin Song,

Rongchi Dai,

Mengran Li

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 290, P. 138927 - 138927

Published: Dec. 19, 2024

Language: Английский

Construction of functional neural network tissue combining CBD-NT3-modified linear-ordered collagen scaffold and TrkC-modified iPSC-derived neural stem cells for spinal cord injury repair DOI Creative Commons

Zhaoping Wu,

Y Zhou, Xianglin Hou

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 35, P. 242 - 258

Published: Feb. 3, 2024

Induced pluripotent stem cells (iPSCs) can be personalized and differentiated into neural (NSCs), thereby effectively providing a source of transplanted for spinal cord injury (SCI). To further improve the repair efficiency SCI, we designed functional network tissue based on TrkC-modified iPSC-derived NSCs CBD-NT3-modified linear-ordered collagen scaffold (LOCS). We confirmed that transplantation this regenerated neurons synapses, improved microenvironment injured area, enhanced remodeling extracellular matrix, promoted recovery hind limbs in rat SCI model with complete transection. RNA sequencing metabolomic analyses also effect from multiple perspectives revealed its potential mechanism treating SCI. Together, constructed using human iPSCs-derived as seed interaction receptors ligands first time. This therapeutic thus confirming feasibility LOCS valuable direction research.

Language: Английский

Citations

11
Functional biomaterials for modulating the dysfunctional pathological microenvironment of spinal cord injury DOI Creative Commons

Dezun Ma,

Changlong Fu,

Fenglu Li

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 39, P. 521 - 543

Published: May 30, 2024

Spinal cord injury (SCI) often results in irreversible loss of sensory and motor functions, most SCIs are incurable with current medical practice. One the hardest challenges treating SCI is development a dysfunctional pathological microenvironment, which mainly comprises excessive inflammation, deposition inhibitory molecules, neurotrophic factor deprivation, glial scar formation, imbalance vascular function. To overcome this challenge, implantation functional biomaterials at site has been regarded as potential treatment for modulating microenvironment to support axon regeneration, remyelination site, recovery after SCI. This review summarizes characteristics recent advances well technologies used modulate inflammatory regulate reshape revascularization microenvironment. Moreover, technological limitations, challenges, future prospects promote efficient repair also discussed. will aid further understanding

Language: Английский

Citations

10
AAV-mediated VEGFA overexpression promotes angiogenesis and recovery of locomotor function following spinal cord injury via PI3K/Akt signaling DOI Creative Commons
Xin Miao, Junqing Lin, Ang Li

et al.

Experimental Neurology, Journal Year: 2024, Volume and Issue: 375, P. 114739 - 114739

Published: Feb. 22, 2024

Spinal cord injury (SCI) is a disorder of the central nervous system resulting from various factors such as trauma, inflammation, tumors, and other etiologies. This condition leads to impairment in motor, sensory, autonomic functions below level injury. Limitations current therapeutic approaches prompt an investigation into angiogenesis through persistent local expression proangiogenic factors. Here, we investigated whether overexpression adeno-associated virus (AAV)-mediated vascular endothelial growth factor A (VEGFA) mouse SCI promoted locomotor function recovery, phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway was mechanistically involved. Three weeks before SCI, AAV-VEGFA injected at T10 induce VEGFA overexpression. Neurofunctional, histological, biochemical assessments were done determine tissue damage and/or recovery neuromuscular behavioral impairments. Daily injections PI3K/Akt inhibitor LY294002 made assess possible mechanism. dramatically improved ameliorated pathological caused by SCI. Improved motor-evoked potentials hindlimbs more spinal CD31-positive microvessels observed AAV-VEGFA-overexpressing mice. reduced PI3K Akt phosphorylation levels attenuated AAV-VEGFA-related improvements. In conclusion, sustained AAV-mediated can significantly promote ameliorate after contusion model activation signaling pathway.

Language: Английский

Citations

9
Construction of a rodent neural network-skeletal muscle assembloid that simulate the postnatal development of spinal cord motor neuronal network DOI Creative Commons

Haiyang Yu,

Shang‐Bin Yang, Yuanfeng Chen

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 29, 2025

Neuromuscular diseases usually manifest as abnormalities involving motor neurons, neuromuscular junctions, and skeletal muscle (SkM) in postnatal stage. Present vitro models of interactions require a long time lack neuroglia involvement. Our study aimed to construct rodent bioengineered spinal cord neural network-skeletal (NN-SkM) assembloids elucidate the between stem cells (SC-NSCs) SkM their biological effects on development maturation circuits. After coculture with cells, SC-NSCs developed into networks (NNs) exhibited high proportion glutamatergic cholinergic low gamma-aminobutyric acidergic increased expression synaptic markers. In NN-SkM assembloids, acetylcholine receptors were upregulated, generating junction-like structures NNs. The amplitude frequency cell contraction by optogenetic glutamate stimulation blocked tetrodotoxin dizocilpine, respectively, confirming existence multisynaptic process involves secretion neurotrophin-3 insulin growth factor-1 which activate related ERK-MAPK PI3K-AKT signaling pathways Inhibition significantly reduces neuronal differentiation while also decreasing receptor formation cells. brief, simulate composition NNs respond regulatory signals, providing an model for studying

Language: Английский

Citations

1
HSPA1A inhibits pyroptosis and neuroinflammation after spinal cord injury via DUSP1 inhibition of the MAPK signaling pathway DOI Creative Commons
Xuegang He, Bo Deng,

Cangyu Zhang

et al.

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: Feb. 9, 2025

Abstract Background Inflammation and proinflammatory programmed cell death, referred to as pyroptosis, are important causes of poor functional recovery after traumatic spinal cord injury (TSCI). Heat shock protein family A member 1A (HSPA1A) is a molecular chaperone that highly expressed TSCI thought be neuroprotective. However, the mechanisms underlying protective effects HSPA1A unclear. Methods The levels pyroptosis inflammation were determined by enzyme-linked immunosorbent assay (ELISA) western blotting analysis. role in regulating was verified vivo vitro experiments. mechanism elucidated bioinformatics coimmunoprecipitation analyses. Results Pyroptosis significantly increased TSCI. overexpression microglia attenuated nigericin- lipopolysaccharide (LPS)-induced vitro, whereas knockdown aggravated inflammation. In vivo, reduced tissue damage, nerve rats promoted recovery. Mechanistically, we identified interacts with dual specificity phosphatase 1 (DUSP1) inhibits activation mitogen-activated kinase (MAPK) pathway, thereby attenuating Conclusion reduces upregulating DUSP1 inhibiting MAPK pathway activation. has potential therapeutic approach promote

Language: Английский

Citations

1
Towards Metahospital: augmented and virtual reality in medicine DOI Open Access
Yevgeniya Daineko, Madina Ipalakova, Dana Tsoy

et al.

Procedia Computer Science, Journal Year: 2024, Volume and Issue: 231, P. 373 - 378

Published: Jan. 1, 2024

Augmented and virtual reality technologies are actively making their way into the field of healthcare, revolutionizing traditional approaches to diagnosis, prevention, treatment. This article discusses how these concepts combined profound impact on emergence Metahospital. The authors provide an overview this concept, highlighting its dual role in aiding healthcare professionals empowering patients. Central paradigm shift is technology's potential enhance specialized training elevate precision efficiency diagnostics, all while prioritizing patient safety. model's core features expounded upon, with a keen emphasis ethical considerations safeguarding personal data, critical aspect explored within paper. Furthermore, elaborates creation surgical simulator tailored for procedures like angioplasty coronary artery stenting, showcasing tangible advancements realm medical technology.

Language: Английский

Citations

8
Microenvironment-responsive nanomedicines: a promising direction for tissue regeneration DOI Creative Commons
Yuan Xiong, Bobin Mi, Mohammad‐Ali Shahbazi

et al.

Military Medical Research, Journal Year: 2024, Volume and Issue: 11(1)

Published: Oct. 21, 2024

Abstract Severe tissue defects present formidable challenges to human health, persisting as major contributors mortality rates. The complex pathological microenvironment, particularly the disrupted immune landscape within these defects, poses substantial hurdles existing regeneration strategies. However, emergence of nanobiotechnology has opened a new direction in immunomodulatory nanomedicine, providing encouraging prospects for and restoration. This review aims gather recent advances nanomedicine foster regeneration. We begin by elucidating distinctive features local microenvironment defective tissues its crucial role Subsequently, we explore design functional properties nanosystems. Finally, address clinical translation development, aiming propose potent approach enhance through synergistic modulation integration.

Language: Английский

Citations

8
Grafted human-induced pluripotent stem cells-derived oligodendrocyte progenitor cells combined with human umbilical vein endothelial cells contribute to functional recovery following spinal cord injury DOI Creative Commons
Qian Li, Sumei Liu, Tianqi Zheng

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 7, 2024

Spinal cord injury (SCI) is a devastating disease that causes extensive damage to oligodendrocytes and neurons leading demyelination axonal degeneration. In this study, we co-transplanted cell grafts containing oligodendrocyte progenitor cells (OPCs) derived from human-induced pluripotent stem (iPSCs) combined with human umbilical vein endothelial (HUVECs), which were reported promote OPCs survival migration, into rat contusion models functional recovery after SCI.

Language: Английский

Citations

6
Biomimetic piezoelectric hydrogel system for energy metabolism reprogramming in spinal cord injury repair DOI Creative Commons
Guoliang Shi,

Tianqi Su,

Junyang Li

et al.

Theranostics, Journal Year: 2025, Volume and Issue: 15(11), P. 4955 - 4969

Published: March 31, 2025

Rationale: Spinal cord injury (SCI) leads to limited regenerative capacity and severe energy deficiency in the microenvironment. This study aimed develop a biomimetic piezoelectric hydrogel system that could recapitulate native tissue microenvironment while enabling wireless physical regulation for SCI repair. Methods: A was fabricated by integrating K0.5Na0.5NbO3 (KNN) nanoparticles with porous decellularized spinal matrix gel (pDG). The hydrogel's effects on vascular endothelial cell migration, neural stem differentiation, ATP synthesis were evaluated vitro. RNA sequencing performed identify key regulatory pathways. therapeutic efficacy assessed rat model of hemisection, examining motor function angiogenesis. Results: demonstrated excellent biocompatibility significantly enhanced migration. Under ultrasonic stimulation, promoted differentiation into neurons more effectively than control hydrogels. stimulation increased calcium ion flux, activating Ca2+/Camk2b/PGC-1α signaling axis. In vivo studies showed implantation combined ultrasound improved recovery Conclusion: presents an effective strategy repair through metabolism reprogramming demonstrates promising potential engineering applications.

Language: Английский

Citations

0
Neuronal Dual-Specificity Phosphatase 26 Inhibition via Reactive-Oxygen-Species Responsive Mesoporous-Silica-Loaded Hydrogel for Spinal Cord Injury Repair DOI
Kai Zhang,

Runlin Wen,

Wanrong Ma

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Spinal cord injury (SCI) remains a formidable challenge in biomedical research, as the silencing of intrinsic regenerative signals most spinal neurons results an inability to reestablish neural circuits. In this study, we found that with low axonal regeneration after SCI showed decreased extracellular signal-regulated kinase (ERK) phosphorylation levels. However, expression dual specificity phosphatase 26 (DUSP26)─which negatively regulates ERK phosphorylation─was reduced considerably undergoing spontaneous regeneration. Therefore, developed system named F10@MS@UV-HG integrated DUSP26-specific inhibitor into reactive oxygen species-responsive nanoparticles and embedded them photosensitive hydrogels. This effectively downregulated DUSP26 primary enhanced phosphorylation, ultimately promoting outgrowth. When transplanted mouse model, achieved sustained drug release, specifically targeting DUSP26/ERK/ELK1 pathway facilitating short-term Additionally, long-term repair effects─including improved myelination motor function─were evident mice F10@MS@UV-HG. The suggested activating signaling by modulating could promote functional recovery. Thus, exhibits enormous potential therapeutic approach for patients SCI.

Language: Английский

Citations

0