International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 10745 - 10765
Опубликована: Окт. 1, 2024
Tissue
engineering
aims
to
recreate
natural
cellular
environments
facilitate
tissue
regeneration.
Gelatin
methacrylate
(GelMA)
is
widely
utilized
for
its
biocompatibility,
ability
support
cell
adhesion
and
proliferation,
adjustable
mechanical
characteristics.
This
study
developed
a
GelMA
graphene
bioink
platform
at
concentrations
of
1,
1.5,
2
mg/mL
enhance
scaffold
properties
applications.
Advanced Materials,
Год журнала:
2024,
Номер
36(34)
Опубликована: Июнь 11, 2024
The
repair
and
functional
reconstruction
of
bone
defects
resulting
from
severe
trauma,
surgical
resection,
degenerative
disease,
congenital
malformation
pose
significant
clinical
challenges.
Bone
tissue
engineering
(BTE)
holds
immense
potential
in
treating
these
defects,
without
incurring
prevalent
complications
associated
with
conventional
autologous
or
allogeneic
grafts.
3D
printing
technology
enables
control
over
architectural
structures
at
multiple
length
scales
has
been
extensively
employed
to
process
biomimetic
scaffolds
for
BTE.
In
contrast
inert
grafts,
next-generation
smart
possess
a
remarkable
ability
mimic
the
dynamic
nature
native
extracellular
matrix
(ECM),
thereby
facilitating
regeneration.
Additionally,
they
can
generate
tailored
controllable
therapeutic
effects,
such
as
antibacterial
antitumor
properties,
response
exogenous
and/or
endogenous
stimuli.
This
review
provides
comprehensive
assessment
progress
3D-printed
BTE
applications.
It
begins
an
introduction
physiology,
followed
by
overview
technologies
utilized
scaffolds.
Notable
advances
various
stimuli-responsive
strategies,
efficacy,
applications
are
discussed.
Finally,
highlights
existing
challenges
development
implementation
scaffolds,
well
emerging
this
field.
Materials Today Bio,
Год журнала:
2025,
Номер
31, С. 101502 - 101502
Опубликована: Янв. 19, 2025
DNA-based
hydrogels
stand
out
for
bone
regeneration
due
to
their
exceptional
biocompatibility
and
programmability.
These
facilitate
the
formation
of
spatial
structures
through
bulk
hydrogel
fabricating,
microsphere
formatting,
3D
printing.
Furthermore,
microenvironment
can
be
finely
tuned
by
leveraging
degradation
products,
nanostructure,
targeting,
delivery
capabilities
inherent
materials.
In
this
review,
we
underscore
advantages
hydrogels,
detailing
composition,
gelation
techniques,
structure
optimization.
We
then
delineate
three
critical
elements
in
promotion
using
hydrogels:
(i)
osteogenesis
driven
phosphate
ions,
plasmids,
oligodeoxynucleotides
(ODNs)
that
enhance
mineralization
promote
gene
protein
expression;
(ii)
vascularization
facilitated
tetrahedral
DNA
nanostructures
(TDNs)
aptamers,
which
boosts
expression
targeted
release;
(iii)
immunomodulation
achieved
loaded
factors,
TDNs,
bound
ions
stimulate
macrophage
polarization
exhibit
antibacterial
properties.
With
these
properties,
used
construct
organoids,
providing
an
innovative
tool
disease
modeling
therapeutic
applications
tissue
engineering.
Finally,
discuss
current
challenges
future
prospects,
emphasizing
potential
impacts
regenerative
medicine.
Materials & Design,
Год журнала:
2024,
Номер
244, С. 113145 - 113145
Опубликована: Июль 8, 2024
The
repair
of
secondary
critical
bone
defects
is
an
international
medical
challenge.
Bone
tissue
engineering
provides
methods
and
technology
for
repair.
regeneration
mechanism
serves
as
inspiration
the
material
structural
design
scaffolds.
In
terms
materials,
this
review
draws
from
biological
characteristics
host
cells
in
osteogenic
microenvironment
(including
osteoblast
lineage,
vascular
cell
inflammatory
cells,
etc.),
reviewing
regulatory
mechanisms
self-healing
proposing
autonomous
living
materials
scaffolds
which
prepared
by
in-situ
manufacturing.
Autonomous
regulate
migration,
proliferation
differentiation
real
time
releasing
steadily
long-term.
Regarding
structure,
we
functional
role
natural
structures
homeostasis,
providing
insights
into
bone-inspired
Due
to
conflict
between
mechanical
properties
ability,
proposes
assembled
They
can
prolong
half-life
provide
support
attachment
points
new
growth,
autonomously
microenvironment.
have
potential
advance
research
progress
field
pave
way
novel
clinical
treatments.
Abstract
Current
two-dimensional
(2D)
cell
models
for
effective
drug
screening
suffer
from
significant
limitations
imposed
by
the
lack
of
realism
in
physiological
environment.
Three-dimensional
(3D)
organoids
hold
immense
potential
mimicking
key
functions
human
organs
overcoming
traditional
2D
models.
However,
current
techniques
preparation
3D
had
reproducibility,
scalability,
and
ability
to
closely
replicate
complex
microenvironment
found
vivo
.
Additionally,
culture
systems
often
involve
lengthy
labor-intensive
processes
that
hinder
high-throughput
applications
necessary
a
large-scale
screening.
Advancements
bioprinting
technologies
offer
promising
solutions
these
challenges
enabling
precise
spatial
control
over
placement
material
composition,
thereby
facilitating
creation
more
physiologically
relevant
than
techniques.
This
review
provides
comprehensive
summary
recent
advances
creating
models,
which
begins
with
an
introduction
different
types
(especially
focus
on
volumetric
(VBP)
technique),
followed
overview
bioinks
utilized
bioprinting.
Moreover,
we
also
introduce
disease
efficiency
evaluation
regenerative
medicine.
Finally,
possible
strategies
development
clinical
translation
are
concluded.
