Pre-treated Mesenchymal Stem Cell-Derived Exosomes: A New Perspective for Accelerating Spinal Cord Injury Repair
European Journal of Pharmacology,
Год журнала:
2025,
Номер
992, С. 177349 - 177349
Опубликована: Фев. 5, 2025
Spinal
cord
injury
(SCI)
is
a
devastating
event
for
the
central
nervous
system
(CNS),
often
resulting
in
loss
of
sensory
and
motor
functions.
It
profoundly
affects
both
physiological
psychological
well-being
patients,
reducing
their
quality
life
while
also
imposing
significant
economic
pressure
on
families
healthcare
system.
Due
to
complex
pathophysiology
SCI,
effective
treatments
promoting
recovery
remain
scarce.
Mesenchymal
stem
cell-derived
exosomes
(MSC-Exos)
offer
advantages
such
as
low
immunogenicity,
good
biocompatibility,
ability
cross
blood-spinal
barrier
(BSCB).
In
preclinical
studies,
they
have
progressively
shown
efficacy
SCI
repair
functional
recovery.
However,
yield
insufficient
targeting
MSC-Exos
limit
therapeutic
efficacy.
Currently,
genetic
engineering
other
preprocessing
techniques
are
being
employed
optimize
properties
exosomes,
thereby
enhancing
potential.
Therefore,
this
paper
provides
an
overview
biogenesis
exosomes.
summarizes
current
approaches
optimizing
exosome
performance.
Additionally,
it
details
mechanisms
through
which
optimized
provide
neuroprotection
explores
potential
combined
involving
hydrogels.
Язык: Английский
Bioprinted Hydrogels as Vehicles for the Application of Extracellular Vesicles in Regenerative Medicine
Gels,
Год журнала:
2025,
Номер
11(3), С. 191 - 191
Опубликована: Март 8, 2025
Three-dimensional
bioprinting
is
a
new
advance
in
tissue
engineering
and
regenerative
medicine.
Bioprinting
allows
manufacturing
three-dimensional
(3D)
structures
that
mimic
tissues
or
organs.
The
bioinks
used
are
mainly
made
of
natural
synthetic
polymers
must
be
biocompatible,
printable,
biodegradable.
These
may
incorporate
progenitor
cells,
favoring
graft
implantation
regeneration
injured
tissues.
However,
the
natures
biomaterials,
processes,
lack
vascularization,
immune
responses
factors
limit
viability
functionality
implanted
cells
damaged
limitations
can
addressed
by
incorporating
extracellular
vesicles
(EV)
into
bioinks.
Indeed,
EV
from
have
capacities,
being
similar
to
those
their
source
cells.
Therefore,
combinations
with
biomaterials
cell-free
therapies.
Likewise,
they
complement
manufacture
increasing
viability,
differentiation,
ability
incorporated
Thus,
main
objective
this
review
show
how
use
3D
technology
for
application
medicine
these
nanovesicles
hydrogels
as
To
end,
latest
advances
derived
vitro
vivo
studies
been
described.
Together,
high
therapeutic
potential
strategy
Язык: Английский
3D bioprinted alginate/gelatin hydrogel: concentration modulated properties toward scar-minimized wound healing
Journal of Biomaterials Science Polymer Edition,
Год журнала:
2025,
Номер
unknown, С. 1 - 22
Опубликована: Апрель 16, 2025
The
critical
shortage
of
transplantable
skin
remains
a
leading
cause
mortality
in
patients
with
severe
injuries,
driving
the
demand
for
advanced
3D-bioprinted
constructs.
While
hydrogel-based
bioinks
are
pivotal
tissue
engineering,
existing
systems
often
fail
to
simultaneously
address
biomechanical
compatibility,
scar
suppression,
and
cell
viability.
Here,
we
propose
rationally
designed
sodium
alginate/gelatin
(SA/Gel)
hydrogel
platform
through
composition-property-performance
correlation
analysis.
Systematic
characterization
revealed
that
increasing
gelatin
content
(8-12
wt%)
enhanced
viscosity
(by
2.5-fold),
compressive
modulus
(25.6
±
2.7
kPa
37.9
3.5
kPa),
tensile
fracture
elongation
(57.9
4.2%
92.1
1.3%),
print
fidelity,
while
reducing
degradation
ratio
(62.8
2.9%
26.4
2.4%
at
day
14)
pore
size
(128.5
16.6
μm
79.4
19.7
μm).
optimized
A4G10
formulation
exhibited
synergistic
advantages:
(1)
dynamic
swelling
(36.3
0.8%)
balanced
nutrient
permeation
structural
stability;
(2)
tunable
(47.2%
matched
neo-tissue
formation;
(3)
anisotropic
mechanical
properties
(compressive
32.2
4.1
kPa,
31.7
3.9
kPa)
mimicked
native
mechanics;
(4)
sub-100
porous
architecture
(102.9
12.4
μm)
effectively
suppressed
fibroblast
over--proliferation.
Remarkably,
SA/Gel
scaffolds
maintained
98%
viability
(Live/Dead
assay)
vitro,
suppressing
fibrotic
formation
facilitating
angiogenesis
vivo.
This
multi-functional
system
demonstrates
unprecedented
potential
as
scar--inhibiting
bioink
clinical-grade
regeneration.
Язык: Английский