Polish Journal of Veterinary Sciences,
Год журнала:
2023,
Номер
unknown
Опубликована: Июнь 28, 2023
Salmonella
contamination
in
poultry
feed
is
one
of
the
main
issues
industry
and
public
health.
The
aim
present
study
was
molecular
detection
typing
serotypes
isolated
from
feeds.
Moreover,
we
determined
antibiotic
resistance
pattern
ability
biofilm
formation
serotypes.
To
this
end,
eighty
samples
were
collected
aviculture
depots.
identified
by
culture
PCR
methods.
For
serological
identification,
a
slide
agglutination
test
used.
BOXAIR
rep-PCR
methods
applied
to
evaluate
diversity
disc
diffusion
method
performed
susceptibility
sixteen
antibiotics.
Biofilm
also
assessed
microtiter-plate
test.
From
total
80
samples,
30
contaminated
with
spp.,
which
divided
into
5
different
belonging
B,
C,
D
serogroups.
BOXAIR-PCR
(D
value
[DI]
0.985)
(DI
0.991)
fingerprinting
isolates
revealed
23
19
reproducible
fingerprint
patterns,
respectively.
A
higher
observed
ampicillin
doxycycline
(100%
each),
followed
chloramphenicol
(83.33%)
tetracycline
(73.33%).
Multidrug
(MDR)
detected
all
Half
possessed
varied
adhesion
strengths.
These
results
high
unexpected
prevalence
MDR
ability.
feeds
subsequently
indicated
variation
source
spp.
unknown
sources
harboring
poor
control,
could
cause
problems
for
manufacturing.
Journal of Dairy Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
This
review
aimed
to
assess
the
scope
of
literature
on
tracking
microbial
community
biofilms,
focusing
dairy
farm
and
processing
environments.
The
majority
studies
focused
either
production,
storage,
transport
or
milk,
while
5
combined
investigation
both
production
facilities.
Factors
influencing
short-term
changes
in
microbiota
such
as
occurrence
mastitis
season
were
distinguished
from
factors
revealed
through
long-term
studies,
feed
weather,
rather
than
milking
equipment.
Knowledge
gaps
identified
relation
study
design,
methods,
data
analysis
interpretation.
application
DNA
sequencing
technologies
is
particularly
challenging
with
respect
samples
low
load
(milk,
swabs).
There
are
few
composition
situ
which
might
require
new
for
detection
before
sampling.
Fundamental
structure
biofilms
needed
identify
on-farm
practices
impacting
cycle
biofilm
development
systems.
Biofilm,
Год журнала:
2023,
Номер
5, С. 100109 - 100109
Опубликована: Фев. 24, 2023
Astronauts
are
spending
longer
periods
locked
up
in
ships
or
stations
for
scientific
and
exploration
spatial
missions.
The
International
Space
Station
(ISS)
has
been
inhabited
continuously
more
than
20
years
the
duration
of
space
stays
by
crews
could
lengthen
with
objectives
human
presence
on
moon
Mars.
If
environment
these
habitats
is
designed
comfort
astronauts,
it
also
conducive
to
other
forms
life
such
as
embarked
microorganisms.
latter,
most
often
associated
surfaces
form
biofilm,
have
implicated
significant
degradation
functionality
pieces
equipment
habitats.
recent
research
suggests
that
microgravity
increase
persistence,
resistance
virulence
pathogenic
microorganisms
detected
communities,
endangering
health
astronauts
potentially
jeopardizing
long-duration
manned
In
this
review,
we
describe
mechanisms
dynamics
installation
propagation
microbial
communities
(spatial
migration),
well
long-term
processes
adaptation
evolution
extreme
environments
(phenotypic
genetic
special
reference
health.
We
discuss
means
control
envisaged
allow
a
lasting
cohabitation
between
vibrant
microscopic
passengers
astronauts.
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Ноя. 20, 2023
Bacillus
subtilis
can
form
various
types
of
spatially
organised
communities
on
surfaces,
such
as
colonies,
pellicles
and
submerged
biofilms.
These
share
similarities
differences,
phenotypic
heterogeneity
has
been
reported
for
each
type
community.
Here,
we
studied
spatial
transcriptional
across
the
three
surface-associated
communities.
Using
RNA-seq
analysis
different
regions
or
populations
community
type,
identified
genes
that
are
specifically
expressed
within
selected
population.
We
constructed
fluorescent
fusions
17
these
genes,
observed
their
expression
in
biofilms
using
time-lapse
confocal
laser
scanning
microscopy
(CLSM).
found
mosaic
patterns
some
genes;
particular,
segregated
cells
displaying
opposite
regulation
carbon
metabolism
(gapA
gapB),
indicative
distinct
glycolytic
gluconeogenic
regimes
coexisting
same
biofilm
region.
Overall,
our
study
provides
a
direct
comparison
heterogeneity,
at
scales,
main
models
B.
Critical Reviews in Food Science and Nutrition,
Год журнала:
2022,
Номер
64(3), С. 654 - 669
Опубликована: Авг. 11, 2022
Hygiene
programs
which
comprise
the
cleaning
and
sanitization
steps
are
part
of
Good
Practices
(GHP)
considered
essential
to
ensure
food
safety
quality.
Inadequate
hygiene
practices
may
contribute
occurrence
foodborne
diseases,
development
microbial
resistance
sanitizers,
economic
losses.
In
general,
sanitizer
is
classified
as
intrinsic
or
acquired.
The
former
an
inherent
characteristic,
naturally
present
in
some
microorganisms,
whereas
latter
linked
genetic
modifications
that
can
occur
at
random
after
continuous
exposure
a
nonnormal
condition.
mechanisms
involve
changes
membrane
permeability
efflux
pump,
enzymatic
activity.
pump
mechanism
most
elucidated
relation
caused
by
use
different
types
sanitizers.
addition,
sanitizers
also
be
favored
presence
biofilms
due
protection
given
glycocalyx
matrix
changes.
Therefore,
this
review
aimed
show
main
including
modifications,
biofilm
formation,
barrier.
Microorganisms,
Год журнала:
2023,
Номер
11(11), С. 2797 - 2797
Опубликована: Ноя. 17, 2023
The
increasing
misuse
of
antibiotics
in
human
and
veterinary
medicine
agroecosystems
the
consequent
selective
pressure
resistant
strains
lead
to
multidrug
resistance
(AMR),
an
expanding
global
phenomenon.
Indeed,
this
phenomenon
represents
a
major
public
health
target
with
significant
clinical
implications
related
increased
morbidity
mortality
prolonged
hospital
stays.
current
presence
microorganisms
multi-resistant
isolated
patients
is
problem
because
additional
burden
disease
it
places
on
most
fragile
difficulty
finding
effective
therapies.
In
recent
decades,
international
organizations
like
World
Health
Organization
(WHO)
European
Centre
for
Disease
Prevention
Control
(ECDC)
have
played
roles
addressing
issue
AMR.
ECDC
estimates
that
Union
alone,
antibiotic
causes
33,000
deaths
approximately
880,000
cases
disability
each
year.
epidemiological
impact
AMR
inevitably
also
has
direct
economic
consequences
not
only
loss
life
but
reduction
number
days
worked,
use
healthcare
resources
diagnostic
procedures
second-line
when
available.
2015,
WHO,
recognising
as
complex
can
be
addressed
by
coordinated
multi-sectoral
interventions,
promoted
One
approach
considers
human,
animal,
environmental
integrated
manner.
review,
authors
try
address
why
collaboration
all
stakeholders
involved
growth
management
necessary
order
achieve
optimal
people,
animals,
plants,
environment,
highlighting
growing
threat
animal
health,
food
safety
security,
prosperity,
ecosystems
worldwide.
