A simple and robust serum-free media for the proliferation of muscle cells

Food Research International, Год журнала: 2023, Номер 172, С. 113194 - 113194

Опубликована: Июнь 29, 2023

Cultivated meat production requires an efficient, robust and highly optimized serum-free cell culture media for the needed upscaling of muscle expansion. Existing formulations are complex, expensive have not been cells. Thus, we undertook this work to develop a simple proliferation bovine satellite cells (SCs) through Design Experiment (DOE) Response Surface Methodology (RSM) using precise high-throughput image-based cytometry. Proliferative attributes were investigated with transcriptomics long-term performance was validated multiple live assays. Here formulated based on three components; FGF2 (2 ng/mL), fetuin (600 µg/mL) BSA (75 which together insulin-transferrin-selenium (1x) supplement, sustained SCs, porcine SCs murine C2C12 Remarkably, cultured in our named Tri-basal 2.0+ performed better than 10% FBS, respect proliferation. Hence, enhanced attachment proliferation, providing alternative solution use FBS cultivated meat.

Язык: Английский

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Cell culture medium cycling in cultured meat: Key factors and potential strategies

Trends in Food Science & Technology, Год журнала: 2023, Номер 138, С. 564 - 576

Опубликована: Июль 8, 2023

Язык: Английский

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Toward sustainable culture media: Using artificial intelligence to optimize reduced-serum formulations for cultivated meat

The Science of The Total Environment, Год журнала: 2023, Номер 894, С. 164988 - 164988

Опубликована: Июнь 19, 2023

Язык: Английский

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Biofabrication's Contribution to the Evolution of Cultured Meat

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(13)

Опубликована: Фев. 10, 2024

Cultured Meat (CM) is a growing field in cellular agriculture, driven by the environmental impact of conventional meat production, which contributes to climate change and occupies ≈70% arable land. As demand for alternatives rises, research this area expands. CM production relies on tissue engineering techniques, where limited number animal cells are cultured vitro processed create meat-like comprising muscle adipose components. Currently, primarily produced small scale pilot facilities. Producing large cell mass based suitable sources bioreactors remains challenging. Advanced manufacturing methods innovative materials required subsequently process into products scale. Consequently, closely linked with biofabrication, suite technologies precisely arranging aggregates cell-material composites construct specific structures, often using robotics. This review provides insights contemporary biomedical biofabrication technologies, focusing significant advancements production. Novel biofabricating also discussed, emphasizing their edibility incorporation healthful Finally, initial studies biofabricated examined, addressing current limitations future challenges large-scale

Язык: Английский

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Microbial lysates as low-cost serum replacements in cellular agriculture media formulation

Food Research International, Год журнала: 2025, Номер 201, С. 115633 - 115633

Опубликована: Янв. 5, 2025

Язык: Английский

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Cultured meat production: what we know, what we don’t know and what we should know

Italian Journal of Animal Science, Год журнала: 2023, Номер 22(1), С. 749 - 753

Опубликована: Авг. 6, 2023

Certain companies in the rapidly expanding cultured meat space claim that is exactly same taste, flavour and nutrition as traditional meat, but without need for animal slaughter, bringing benefits human health environment. It important to point out still early phases of development and, many cases, claims are based on assumptions rather than facts. In this work, we outline several knowledge gaps, mainly technical issues, with repercussions related possible well economic regulatory challenges.

Язык: Английский

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Recent advances in scaffolding biomaterials for cultivated meat

Biomaterials Advances, Год журнала: 2024, Номер 162, С. 213897 - 213897

Опубликована: Май 21, 2024

Язык: Английский

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Non-animal protein hydrolysates from agro-industrial wastes: A prospect of alternative inputs for cultured meat

Food Chemistry, Год журнала: 2024, Номер 443, С. 138515 - 138515

Опубликована: Янв. 23, 2024

Язык: Английский

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Current Research, Industrialization Status, and Future Perspective of Cultured Meat

Food Science of Animal Resources, Год журнала: 2024, Номер 44(2), С. 326 - 355

Опубликована: Фев. 2, 2024

Expectations for the industrialization of cultured meat are growing due to increasing support from various sectors, such as food industry, animal welfare organizations, and consumers, particularly vegetarians, but progress is slower than initially reported. This review analyzes main issues concerning meat, examines research media reports on development date, presents current technology, level, prospects meat. Currently, over 30 countries have companies industrializing around 200 that developing or been surveyed globally. By country, United States has 50 companies, accounting more 20% total. Acquiring cells, cell lines, improving proliferation, efficiency differentiation muscle production, culture media, including serum-free major themes related In contrast, devices, bioreactors, which crucial in enabling large-scale relatively understudied, few many invested presented products sale other prototypes. addition, because most information key technologies not publicly available, it possible determine level technology surmised meat-related startups high. Therefore, further needed promote full-scale

Язык: Английский

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Considerations for cultivated crustacean meat: potential cell sources, potential differentiation and immortalization strategies, and lessons from crustacean and other animal models

Critical Reviews in Food Science and Nutrition, Год журнала: 2024, Номер unknown, С. 1 - 25

Опубликована: Май 11, 2024

Cultivated crustacean meat (CCM) is a means to create highly valued shrimp, lobster, and crab products directly from stem cells, thus removing the need farm or fish live animals. Conventional enterprises face increasing pressures in managing overfishing, pollution, warming climate, so CCM may provide way ensure sufficient supply as global demand for these grows. To support development of CCM, this review briefly details cell culture work date, before addressing what presently known about muscle development, particularly molecular mechanisms involved, how might relate recent on cultivated production vertebrate species. Recognizing current lack lines available establish cultures, we also consider primary sources that can be obtained non-lethally including tissues limbs which are readily released regrown, putative cells circulating hemolymph. Molecular approaches inducing myogenic differentiation immortalization reviewed. Finally, assess status tools researchers, antibodies, propose avenues address existing shortfalls order see field progress.

Язык: Английский

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