IntechOpen eBooks,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 28, 2024
Adipose
tissue
mesenchymal
stem
cells
(AD-MSC
or
ADSC)
are
multipotent
that
do
not
show
immune
rejection.
In
this
work,
we
analyze
the
route
of
administration
and
its
possible
differentiation
into
specific
lineages
adipogenic,
chondrogenic,
osteogenic,
myogenic,
neurogenic
phenotypes.
Transplanted
induced
repair
by
inducing
angiogenic,
anti-inflammatory,
immunomodulatory
effects
(IDO,
PG-2,
nitric
oxide,
some
cytokine
signaling).
The
ADSC
exert
these
processes
through
release
chemokines
growth
factors
in
a
paracrine
manner.
Other
fat-derived
such
as
perivascular
adipose
(PVAT)
muse
reparative
effects.
Cell-free
therapy
using
stromal
vascular
fraction
(SVF)
use
exosomes
releasing
miRNAs
cytokines
also
confirmed
their
safety
efficacy
vitro.
Several
published
preclinical
clinical
trials
with
AD-MSC
beneficial
to
prevent
chronic-degenerative
pathologies.
chapter,
review
AD-MSC-based
therapies
have
used
rodent
models
disease
for
cartilage
repair,
regeneration
peripheral
central
nervous
system,
dental
bone,
myocardium,
liver,
treatment
perianal
fistula
Chron’s
disease,
wound
skin
fibrosis
repair.
addition,
work
includes
studies
other
patients
various
Journal of Extracellular Vesicles,
Год журнала:
2024,
Номер
13(7)
Опубликована: Июль 1, 2024
Abstract
Extracellular
vesicles
(EVs)
derived
from
dental
pulp
stem
cells
(DPSC)
have
been
shown
an
excellent
efficacy
in
a
variety
of
disease
models.
However,
current
production
methods
fail
to
meet
the
needs
clinical
treatment.
In
this
study,
we
present
innovative
approach
substantially
enhance
‘Artificial
Cell‐Derived
Vesicles
(ACDVs)’
by
extracting
and
purifying
contents
released
DPSC
lysate,
namely
intracellular
vesicles.
Comparative
analysis
was
performed
between
ACDVs
those
obtained
through
ultracentrifugation.
The
extracted
cell
lysate
general
standard
EVs
similar
protein
secretion
profile.
new
also
significantly
promoted
wound
healing,
increased
or
decreased
collagen
regeneration,
reduced
inflammatory
factors
as
EVs.
More
importantly,
extraction
efficiency
is
improved
16
times
compared
with
using
ultracentrifuge
method.
With
its
impressive
attributes,
subtype
emerge
prospective
candidate
for
future
applications
regenerative
medicine.
Abstract
Critical-sized
bone
defects
represent
a
significant
clinical
challenge
due
to
their
inability
undergo
spontaneous
regeneration,
necessitating
graft
interventions
for
effective
treatment.
The
development
of
tissue-engineered
scaffolds
and
regenerative
medicine
has
made
tissue
engineering
highly
viable
treatment
defects.
physical
biological
properties
nanocomposite
biomaterials,
which
have
optimized
structures
the
ability
simulate
microenvironment
bone,
are
promising
application
in
field
engineering.
These
biomaterials
offer
distinct
advantages
over
traditional
materials
by
facilitating
cellular
adhesion
proliferation,
maintaining
excellent
osteoconductivity
biocompatibility,
enabling
precise
control
degradation
rates,
enhancing
mechanical
properties.
Importantly,
they
can
natural
structure
tissue,
including
specific
microenvironment,
is
crucial
promoting
repair
regeneration
This
manuscript
provides
comprehensive
review
recent
research
developments
applications
structure-optimized
microenvironment-inspired
focuses
on
these
summarizing
latest
progress
highlighting
challenges
future
perspectives
field.
Through
this
analysis,
paper
aims
underscore
potential
engineering,
contributing
informed
design
strategic
planning
next-generation
medicine.
Biomaterials Research,
Год журнала:
2025,
Номер
29
Опубликована: Янв. 1, 2025
Segmental
defects
of
long
bones
are
among
the
most
challenging
and
debilitating
conditions
in
clinical
medicine.
Osteogrow-C
is
a
novel
osteoinductive
device
composed
recombinant
human
bone
morphogenetic
protein
6
(rhBMP6)
delivered
within
autologous
blood
coagulum
(ABC)
with
calcium
phosphate
ceramics
that
was
proven
efficacious
preclinical
models
spinal
fusion.
This
study
aimed
to
evaluate
efficacy
comparison
other
therapies
clinically
relevant
segmental
defect
model
rabbits.
(15
mm)
rabbit
ulna
were
treated
containing
different
synthetic
ceramic
particles
(tricalcium
[TCP]
TCP/hydroxyapatite
40%/60%),
Osteogrow
(rhBMP6/ABC),
Infuse
(rhBMP2/absorbable
collagen
sponge),
control
implants
without
proteins.
Defect
healing
evaluated
by
vivo
x-ray
scans
4,
8,
17
weeks
after
surgery,
animals
killed
for
further
radiographical
histological
assessment.
Evaluation
images,
micro-computed
tomography,
sections
revealed
both
formulations
as
well
promoted
ulnar
defect.
However,
radiographic
scores
higher
than
those
used
therapies.
Moreover,
evaluation
TCP
induced
rapid
bridging.
On
hand,
(ABC/TCP
ABC/biphasic
phosphate)
limited
osteogenesis
The
findings
this
suggest
promising
safe
therapeutic
solution
treatment
large
defects,
providing
relief
millions
patients
suffering
from
condition.
Biomacromolecules,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 22, 2025
Silk
fibroin
(SF)
hydrogels
are
widely
used
in
three-dimensional
(3D)
cell
culture
and
tissue
repair.
Despite
their
importance,
few
studies
have
focused
on
regulating
degradation
further
revealing
the
effects
of
process
encapsulated
behaviors.
Herein,
SF
with
equivalent
initial
properties
different
rates
were
prepared
by
adjusting
ratios
between
hydrogel-encapsulated
normal
microspheres
(MSN)
enzyme-loaded
(MSE).
Further,
experiments
revealed
that
moderately
accelerating
hydrogel
obviously
improved
proliferation
MSCs
during
7
days
culture.
Slightly
promoted
MSC
chondrogenesis.
However,
too
rapid
a
was
unfavorable
for
these
The
relevant
expected
to
provide
useful
strategies
also
afford
new
references
development
excellent
other
protein-based
biomaterials
cartilage
regeneration.
Journal of Applied Polymer Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 27, 2025
ABSTRACT
Artificial
implants
are
necessary
for
orthopedic
treatment
and
the
recovery
of
bone
joint
function
in
disabled
individuals.
However,
existing
materials
present
challenges
manufacturing
complex
structures
limited
terms
mechanical
frictional
properties.
Current
research
is
focused
on
development
new
environmentally
friendly
polymer
composites
manufacture
artificial
implants,
combined
with
3D
printing
technology
to
improve
design
flexibility.
In
this
study,
nine
kinds
PLA/n‐HA/PA66
composite
scaffolds
different
proportions
were
prepared
using
light‐curing
technology.
The
physical,
mechanical,
tribological
properties
compared.
results
showed
that
scaffold
was
superior
traditional
polymers,
compressive
strength
increased
by
30–40
MPa
wear
resistance
27%–64%.
This
indicates
it
has
wide
application
potential
tissue
engineering.
Advanced Engineering Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 29, 2025
Over
the
past
two
decades,
remarkable
advancements
have
been
achieved
in
stimulus‐responsive
shape‐memory
polymers
(SMPs),
which
exhibit
desirable
properties
such
as
characteristics,
deformability,
and
biocompatibility,
while
responding
to
external
stimuli.
The
development
of
polymer
composites
(SMPCs)
leads
high
recovery
forces
novel
functionalities,
including
electrical
actuation,
magnetic
biocompatibility.
enhanced
remotely
controllable
functionality
further
expand
application
SMPs
biomedical
areas,
surgical
applications
for
replacing
handheld
instruments
drug
delivery
systems.
In
this
review,
device
SMPCs
are
focused
on
their
recent
bone
tissue
scaffolds,
lumen
stents,
carriers
examined.
Furthermore,
bottlenecks
challenges
encountered
by
devices
elucidated.
future
trend
is
also
discussed,
aiming
provide
valuable
insights
broadening
fields.
Bile
duct
injuries
remain
a
significant
clinical
challenge
following
hepatobiliary
surgeries.
Recently,
3D-printed
tubular
scaffolds
have
shown
promise
as
effective
solutions
for
bile
tissue
repair.
This
study
presents
novel
bi-layered
scaffold,
fabricated
using
advanced
three-dimensional
printing
technology.
The
inner
layer
of
this
scaffold
is
constructed
from
poly(ε-caprolactone)
methacrylate
(PCLMA),
which
imparts
high
mechanical
strength.
outer
composed
biocompatible
methacrylated
recombinant
type
I
collagen
(rColMA)
and
ε-poly(L-lysine)-methacrylamide
(EPLMA),
providing
excellent
antibacterial
properties.
In
addition,
encapsulated
interleukin-6-loaded
liposomes
(IL-6@Lip)
are
incorporated
into
the
to
further
promote
regeneration.
innovative
design
creates
an
optimal
microenvironment
growth
differentiation
bone
marrow
mesenchymal
stem
cells
(BMSCs)
duct-like
cells.
These
differentiated
contribute
regeneration,
evidenced
by
expression
key
markers,
including
CK7,
CK19,
AQP1,
AE2,
CX43.
rColMA/EPLMA/IL-6@Lip
hydrogel
in
significantly
enhances
BMSC
proliferation
their
epithelial
Furthermore,
vivo
experiments
show
that
PCLMA//rColMA/EPLMA/IL-6@Lip
does
not
induce
stasis.
novel,
pre-differentiated
active
offers
valuable
insights
regeneration
replacement
research.
Chemical Communications,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Extracellular
vesicles
have
been
deemed
as
potential
drug
carriers
for
treatment
of
various
diseases.
Recent
advances
summarized,
including
the
sources,
delivery
function,
extraction
and
cargo-loading
technology
extracellular
vesicles.
