A novel BODIPY-based fluorescent probe for naked-eye detection of the highly alkaline pH

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2024, Номер 325, С. 125083 - 125083

Опубликована: Сен. 6, 2024

Язык: Английский

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Near-Infrared Visualization of NAD(P)H Dynamics in Live Cells and Drosophila melanogaster Larvae Using a Coumarin-Based Pyridinium Fluorescent Probe

ACS Applied Bio Materials, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 19, 2024

A near-infrared fluorescent probe,

Язык: Английский

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Precision Imaging of Biothiols in Live Cells and Treatment Evaluation during the Development of Liver Injury via a Near-Infrared Fluorescent Probe

Chemical & Biomedical Imaging, Год журнала: 2024, Номер 3(3), С. 169 - 179

Опубликована: Авг. 28, 2024

In this study, a biothiol-sensitive near-infrared (NIR) fluorescent sensor, BDP-CYS, based on coumarin-hemicyanine skeleton, was designed and developed thiol–halogen SNAr nucleophilic substitution. BDP-CYS successfully implemented to ratiometrically monitor endogenous exogenous Cys, Hcy, GSH in living cells as well distinguish between normal cancer cells. Furthermore, the probe utilized detect changes of biothiols drug-induced hepatotoxicity evaluate treatment effectiveness diabetes-associated liver injury vivo. The advantages BDP-CYS's include practical sensitivity, high selectivity, rapidity reaction, stability across range pH temperature conditions, thus introducing new tool better understand roles oxidative stress.

Язык: Английский

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An innovative dual-organelle targeting NIR fluorescence probe for detecting hydroxyl radicals in biosystem and inflammation models

Bioorganic Chemistry, Год журнала: 2024, Номер 151, С. 107678 - 107678

Опубликована: Июль 27, 2024

Язык: Английский

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Deep-Red and Near-Infrared Compact Cyanine Dyes for Sensitive NAD(P)H Sensing in Live Cells and Kidney Disease Tissues

ACS Applied Bio Materials, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 26, 2024

Cyanine dyes constructed for NAD(P)H near-infrared sensing utilize extended π-conjugation but often exhibit delayed fluorescence responses to due reduced positive charge density in 3-quinolinium acceptors. This study introduces deep-red and compact cyanine represented by probes A B mitochondrial detection live cells. Probes feature a unique structural design with double bond connection linking strategically positioned 1-methylquinolinium acceptor units at 2- 4-positions, correspondingly. Probe absorbs 359 531 nm, while probe 324 370 emitting subtle 587 628 respectively, no NADH present. Upon exposure, significant emission enhancements 612 656 correspondingly, attributed the efficient reduction of electron-donative 1-methyl-1,4-dihydroquinoline units. B, chosen its fast response NAD(P)H, effectively monitored dynamic intracellular levels throughout diverse experimental conditions. In HeLa cells, minimal basal increased upon stimulation. It also identified following chemical treatments acesulfame potassium, cisplatin, carboplatin, temozolomide, CoCl2-induced hypoxia, TLR4 activation macrophages disease models kidney pathology, where diseased tissues exhibited higher than normal tissues. fruit fly larvae under starvation conditions, tracked increases triggered exogenous NADH, demonstrating vivo applicability metabolic studies. These findings highlight B's utility elucidating fluctuations biological contexts, offering insights into function cellular metabolism.

Язык: Английский

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A coumarin-based red-emitting fluorescent probe for sequential detection of Fe3+ and PPi, and its applications in real water sample and bioimaging in vivo

Journal of Molecular Structure, Год журнала: 2025, Номер unknown, С. 141572 - 141572

Опубликована: Янв. 1, 2025

Язык: Английский

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A Rhodamine-Based Ratiometric Fluorescent Sensor for Dual-Channel Visible and Near-Infrared Emission Detection of NAD(P)H in Living Cells and Fruit Fly Larvae

ACS Applied Bio Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 5, 2025

The detection and dynamic monitoring of intracellular NAD(P)H concentrations are crucial for comprehending cellular metabolism, redox biology, their roles in various physiological pathological processes. To address this need, we introduce sensor A, a near-infrared ratiometric fluorescent real-time, quantitative imaging fluctuations live cells. Sensor A combines 3-quinolinium electron-deficient acceptor with rhodamine dye, offering high sensitivity specificity superior photophysical properties. In its unbound state, emits strongly at 650 nm weakly 465 upon 400 excitation. Upon binding to NAD(P)H, it shows fluorescence increase decrease nm, enabling accurate measurements. also exhibits upconversion when excited 800 or 810 additional flexibility different experimental setups. sensor's response relies on the reduction by forming 1,4-dihydroquinoline donor that enhances quenches emission through photoinduced electron transfer. This mechanism ensures reliable quantification levels while minimizing interference from concentration, excitation intensity, environmental factors. was validated HeLa MD-MB453 cells under metabolic pharmacological conditions, including glucose maltose stimulation treatments chemotherapeutic agents. Co-localization mitochondrial-specific dyes confirmed mitochondrial targeting, precise tracking fluctuations. vivo Drosophila larvae nutrient starvation exposure revealed dose-dependent responses, highlighting potential changes organisms. represents significant advancement imaging, providing powerful tool exploring metabolism biology biomedical research.

Язык: Английский

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An indolium-based near-infrared fluorescent probe for non-invasive real-time monitoring of gastric pH in vitro and in vivo

Analytical Methods, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

A new fluorescent probe with good spectral properties for the in situ imaging of gastric acidity mice is reported.

Язык: Английский

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A coumarin-based fluorescent probe for sequential recognition of zinc ion and nitric oxide, and its applications in real water sample, fluorescence anti-counterfeiting and bioimaging in vivo

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2025, Номер unknown, С. 126405 - 126405

Опубликована: Май 1, 2025

Язык: Английский

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Benzimidazole-Derived B2 as a Fluorescent Probe for Bacterial Outer Membrane Vesicle (OMV) Labeling: Integrating DFT, Molecular Dynamics, Flow Cytometry, and Confocal Microscopy

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(10), С. 4682 - 4682

Опубликована: Май 14, 2025

Bacterial outer membrane vesicles (OMVs) are nanoscale extracellular structures produced by Gram-negative bacteria that critical for microbial biology and host-pathogen interactions have great potential in biotechnological applications. Despite the availability of fluorescent dyes OMV studies, many repurposed from eukaryotic vesicle research not explicitly optimized OMVs, leading to challenges achieving consistent labeling, minimizing background noise, preserving integrity during analyses. This study evaluates B2, a benzimidazole-derived fluorophore, labeling advanced techniques like flow cytometry confocal microscopy. OMVs were isolated Escherichia coli strains BL21 O157, their was confirmed using transmission electron microscopy (TEM). B2 staining protocols fluorescence analyses revealed specific with membranes, reducing aggregation enhancing signal uniformity. Flow indicated near-complete efficiency (98-100%) minimal interference. Confocal further validated B2's effectiveness, showing evident internalization into epithelial HT-29 cells compatibility other fluorophores. Density functional theory (DFT) calculations, including Fukui function analysis, identified key electrophilic nucleophilic regions facilitate hydrogen bonding polar components. Non-covalent interaction (NCI) analysis pronounced intramolecular along discrete weak van der Waals interactions. Molecular dynamics simulations suggest exhibits an affinity both hydrophobic core lipid bilayer oligosaccharide region LPS layer, which collectively ensures sustained retention dye. The findings presented this position as valuable fluorophore research.

Язык: Английский

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