Опубликована: Янв. 1, 2024
Язык: Английский
Advanced Science, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 12, 2024
Abstract Polymicrobial biofilms adhere to surfaces and enhance pathogen resistance conventional treatments, significantly contributing chronic infections in the respiratory tract, oral cavity, wounds, on medical devices. This review examines antimicrobial peptides (AMPs) as a promising alternative traditional antibiotics for treating biofilm‐associated infections. AMPs, which can be produced part of innate immune response or synthesized therapeutically, have broad‐spectrum activity, often disrupting microbial cell membranes causing death. Many specifically target negatively charged bacterial membranes, unlike host membranes. Research shows AMPs effectively inhibit disrupt polymicrobial antibiotics' efficacy. Preclinical clinical research is advancing, with animal studies trials showing promise against multidrug‐resistant bacteria fungi. Numerous patents indicate increasing interest AMPs. However, challenges such peptide stability, potential cytotoxicity, high production costs must addressed. Ongoing focuses optimizing AMP structures, enhancing developing cost‐effective methods. In summary, offer novel approach combating infections, their unique mechanisms synergistic existing positioning them candidates future treatments.
Язык: Английский
Процитировано
16
Bioelectrochemistry, Год журнала: 2024, Номер 160, С. 108784 - 108784
Опубликована: Июль 27, 2024
Язык: Английский
Процитировано
8
ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(25), С. 32087 - 32103
Опубликована: Июнь 12, 2024
Due to the extensive use of antibiotics, many highly resistant bacteria and extensively have been produced. In recent years, increase drug-resistant resulting proliferation increased incidence hospital-acquired infections caused great harm human health. Antimicrobial peptides (AMPs) are considered be an innovative antibiotic belong latest advances in this field. We designed a polypeptide verified its low minimum inhibitory concentration broad-spectrum activity against Gram-positive bacteria, Gram-negative fungi microbiology pharmacology. Several experiments confirmed that screened antimicrobial significant antidrug resistance also show therapeutic properties treatment systemic bacterial infections. addition, through our experimental research, it was proved antibacterial hydrogel composed poly(vinyl alcohol), sodium alginate, had excellent showed good wound healing ability.
Язык: Английский
Процитировано
6
Molecules, Год журнала: 2024, Номер 30(1), С. 128 - 128
Опубликована: Дек. 31, 2024
Multidrug-resistant (MDR) bacteria are becoming more and common, which presents a serious threat to world health could eventually render many of the antibiotics we currently use useless. The research development innovative antimicrobial tactics that can defeat these hardy infections imperative in light this predicament. Antimicrobial peptides (AMPs), have attracted lot attention due their distinct modes action capacity elude conventional resistance mechanisms, among most promising tactics. As substitute for antibiotics, AMPs varied class naturally occurring compounds target membranes disrupt cellular activities demonstrate broad-spectrum activity. objective study is present thorough summary current knowledge regarding AMP mechanisms against MDR bacteria, including immunological modulation, interactions with microbial membranes, possible synergy used drugs. In addition, define review’s scope include recent developments research, emphasizing innovations’ development, optimization, therapeutic promise. We hope emphasize crucial role will play future therapy by bringing together highlighting issues. also advocate AMPs’ continued as part comprehensive strategy counteract growing antibiotic resistance.
Язык: Английский
Процитировано
5
Current Research in Biotechnology, Год журнала: 2024, Номер 8, С. 100240 - 100240
Опубликована: Янв. 1, 2024
The demand for broad-spectrum antibacterial agents continues with increasing rates of resistance microbial pathogens to traditional antibiotics. Peptides and lipopeptides are gaining traction as promising novel, class-reference antibiotics tackling difficult-to-treat infections caused by multi-drug resistant bacteria. To identify novel candidates expand treatment options in clinical settings, we explored the vitro potential mode action a short octapeptide combining cationic block four lysines highly hydrophobic segment phenylalanines (K4F4), two K4F4-inspired (Palmitoyl-K4F4 K4-NH-Palmitoyl). Preliminary AI-based screening had revealed antimicrobial K4F4 peptide coupled limited haemolytic activity. Broth dilution assays have confirmed these silico predictions. Overall, our lipidated peptides were more active at lower MIC values compared non-lipidated species, indicating beneficial impact tailing lipidation on design peptide-based antimicrobials. An integrated view membrane-active mechanism therapeutic templates was obtained using combination flow cytometry, fluorescence microscopy dye-based permeabilization assays. its derivatives act via fast-disrupting without inducing bacterial mechanisms long-term exposure assay. A lipopeptide together shorter version (K4-NH-Palmitoyl), stable environments closer emulating physiological conditions, showing higher response salts serum than their parent peptide. Our findings reveal antibiofilm polylysine-polyphenyalanine highlight significant contribution shortening molecular engineering strategies improve guide future next-generation membrane-targeting
Язык: Английский
Процитировано
4
Bioactive Materials, Год журнала: 2024, Номер 46, С. 97 - 117
Опубликована: Дек. 10, 2024
Язык: Английский
Процитировано
4
Bioorganic Chemistry, Год журнала: 2025, Номер 157, С. 108309 - 108309
Опубликована: Фев. 22, 2025
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
BIO Web of Conferences, Год журнала: 2025, Номер 163, С. 01007 - 01007
Опубликована: Янв. 1, 2025
Bioactive agents are compounds that have an influence on human beings, organs, or tissues. These agents, which might be found in both natural and synthetic chemicals, able to interact with biological systems produce a variety of therapeutic responses. In this regard, work proposes stacking method for categorizing five important bioactive agents: antibacterial, anti-HIV, antioxidant, antiparasitic, antiprotozoal. This study has been designed graph-based feature extraction approach successfully captures intricate interactions between molecular structures substances. extracted characteristics were then put into strategy, is strong ensemble learning technique leverages the capabilities several machine models improve classification accuracy. By utilizing innovative technique, model outperformed state-of-the-art methods across all assessment criteria more than 85% terms The findings demonstrate efficacy conjunction model, making it useful tool detecting chemicals such as antiprotozoal drug development, hence facilitating development novel essential health applications.
Язык: Английский
Процитировано
0
Microorganisms, Год журнала: 2025, Номер 13(3), С. 639 - 639
Опубликована: Март 11, 2025
Acinetobacter (A.) baumannii is a major nosocomial pathogen in human and veterinary medicine. The emergence of certain international clones (ICs), often with multidrug-resistant (MDR) phenotypes biofilm formation (BF), facilitates its spread clinical environments. global rise antimicrobial resistance demands alternative treatment strategies, such as peptides (AMPs). In this study, 45 companion animal MDR-A. isolates, belonging to the globally IC1, IC2 IC7, were tested for biofilm-associated genes (BAGs) their capacity BF. Of these, 13 used test inhibitory effect AMPs on bacterial growth (BG) BF through application crystal violet assay. two novel AMP variants Bac7(17) (target cell inactivation) Pasmr5-17 (efflux pump inhibition) well-known phenylalanine-arginine-β-naphthylamide (PAβN) at concentrations 1.95 1000 µg/mL. Based whole-genome sequence data, identical patterns BAGs detected within same IC. inhibited BG dose-dependent manner. PAsmr5-17 highly effective against BG, inhibition (GI) >99% (62.5 125 µg/mL, respectively). PAβN achieved only 95.7% GI Similar results obtained Differences between ICs found both when influenced by AMPs. had hardly any IC1 but IC7 31.25 µg/mL was sufficient. Our data show that susceptibility most likely resembles depending assignment particular Even low significant BG. Therefore, represent promising baumannii, either sole therapy or combination antibiotics.
Язык: Английский
Процитировано
0