BMC Infectious Diseases,
Год журнала:
2024,
Номер
24(1)
Опубликована: Июль 9, 2024
Abstract
Background
There
is
an
increasing
disease
trend
for
SARS-COV-2,
so
need
a
quick
and
affordable
diagnostic
method.
It
should
be
highly
accurate
save
costs
compared
to
other
methods.
The
purpose
of
this
research
achieve
these
goals.
Methods
This
study
analyzed
342
samples
using
TaqMan
One-Step
RT-qPCR
fast
RT-LAMP
(Reverse
Transcriptase
Loop-Mediated
Isothermal
Amplification).
LAMP
assay
was
conducted
assess
the
sensitivity
specificity.
Results
reported
positive
two
different
In
method,
saliva
had
92
(26.9%)
250
negative
(73.09%)
nasopharynx
94
(27.4%)
248
(72.51%).
86
(25.1%)
256
(74.8%)
93
(27.1%)
249
(72.8%).
agreement
between
tests
in
93%
94%
respectively,
based
on
Cohen’s
kappa
coefficient
(κ)
(
P
<
0.001).
rate
technique
at
dilution
1
×
10
100%
Conclusions
Based
results
has
multiple
advantages.
These
include
simplicity,
cost-effectiveness,
high
sensitivity,
shows
promise
as
tool.
can
help
manage
outbreaks,
ensure
prompt
treatment,
safeguard
public
health
by
providing
rapid,
easy-to-use
testing.
Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 3, 2024
Early-stage
disease
detection,
particularly
in
Point-Of-Care
(POC)
wearable
formats,
assumes
pivotal
role
advancing
healthcare
services
and
precision-medicine.
Public
benefits
of
early
detection
extend
beyond
cost-effectively
promoting
outcomes,
to
also
include
reducing
the
risk
comorbid
diseases.
Technological
advancements
enabling
POC
biomarker
recognition
empower
discovery
new
markers
for
various
health
conditions.
Integration
wearables
with
intelligent
frameworks
represents
ground-breaking
innovations
automation
operations,
conducting
advanced
large-scale
data
analysis,
generating
predictive
models,
facilitating
remote
guided
clinical
decision-making.
These
substantially
alleviate
socioeconomic
burdens,
creating
a
paradigm
shift
diagnostics,
revolutionizing
medical
assessments
technology
development.
This
review
explores
critical
topics
recent
progress
development
1)
systems
solutions
physiological
monitoring,
as
well
2)
discussing
current
trends
adoption
smart
technologies
within
settings
developing
biological
assays,
ultimately
3)
exploring
utilities
platforms
discovery.
Additionally,
translation
from
research
labs
broader
applications.
It
addresses
associated
risks,
biases,
challenges
widespread
Artificial
Intelligence
(AI)
integration
diagnostics
systems,
while
systematically
outlining
potential
prospects,
challenges,
opportunities.
Analytical Methods,
Год журнала:
2023,
Номер
15(18), С. 2154 - 2180
Опубликована: Янв. 1, 2023
POCT
technology
has
shown
promise
for
rapid
diagnosis.
nanoparticle-based
immunoassays
can
be
used
to
detect
different
targets
with
high
sensitivity,
selectivity,
and
affordability.
Clinical Chemistry and Laboratory Medicine (CCLM),
Год журнала:
2023,
Номер
61(12), С. 2102 - 2114
Опубликована: Июнь 14, 2023
An
emerging
technology
(ET)
for
laboratory
medicine
can
be
defined
as
an
analytical
method
(including
biomarkers)
or
device
(software,
applications,
and
algorithms)
that
by
its
stage
of
development,
translation
into
broad
routine
clinical
practice,
geographical
adoption
implementation
has
the
potential
to
add
value
diagnostics.
Considering
medicine-specific
definition,
this
document
examines
eight
key
tools,
encompassing
clinical,
analytical,
operational,
financial
aspects,
used
throughout
life
cycle
ET
implementation.
The
tools
provide
a
systematic
approach
starting
with
identifying
unmet
need
opportunities
improvement
(Tool
1),
forecasting
2),
readiness
assessment
3),
health
4),
organizational
impact
map
5),
change
management
6),
total
pathway
evaluation
checklist
7),
green
procurement
8).
Whilst
there
are
differences
in
priorities
between
different
settings,
use
set
will
help
support
overall
quality
sustainability
Frontiers in Microbiology,
Год журнала:
2022,
Номер
13
Опубликована: Окт. 28, 2022
Pathogenic
microorganisms
have
major
impacts
on
human
lives.
Rapid
and
sensitive
diagnostic
tools
are
urgently
needed
to
facilitate
the
early
treatment
of
microbial
infections
effective
control
transmission.
CRISPR-Cas13
employs
programmable
RNA
produce
a
specific
method
with
high
base
resolution
thus
provide
novel
tool
for
rapid
detection
microorganisms.
The
review
aims
insights
spur
further
development
by
summarizing
characteristics
effectors
system
describing
latest
research
into
its
application
in
pathogenic
combination
nucleic
acid
extraction,
isothermal
amplification,
product
detection.
One Health,
Год журнала:
2023,
Номер
16, С. 100536 - 100536
Опубликована: Апрель 6, 2023
Detection
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
viral
genome
in
wastewater
has
proven
to
be
useful
for
tracking
the
trends
virus
prevalence
within
community.
The
surveillance
also
provides
precise
and
early
detection
any
new
circulating
variants,
which
aids
response
outbreaks.
Site-specific
monitoring
SARS-CoV-2
variants
valuable
information
on
or
emerging
We
sequenced
genomic
RNA
viruses
present
samples
analyzed
as
well
other
a
period
one
year
account
seasonal
variations.
were
collected
from
Reno-Sparks
metropolitan
area
weekly
basis
between
November
2021
2022.
Samples
detect
levels
copies
identification.
This
study
confirmed
that
can
used
community
supports
wastewater-based
epidemiology
(WBE)
complement
clinical
testing
healthcare
effort.
Our
showed
persistence
throughout
compared
presence
viruses,
implicating
SARS-CoV-2's
broad
genetic
diversity
strength
persist
infect
susceptible
hosts.
Through
secondary
analysis,
we
further
identified
antimicrobial
resistance
(AMR)
genes
same
found
WBE
feasible
tool
AMR
monitoring.
Applied Sciences,
Год журнала:
2024,
Номер
14(8), С. 3157 - 3157
Опубликована: Апрель 9, 2024
Producing
sustainable
microfluidic
devices
on
a
large
scale
has
become
trend
in
the
biomedical
field.
However,
transition
from
laboratory
prototyping
to
large-scale
industrial
production
poses
several
challenges
due
gap
between
academia
and
industry.
In
this
context,
with
mass
approach
could
be
novel
strategy
necessary
bridge
academic
research
market.
Here,
performance
of
polymer
inserts
produce
PMMA
using
microinjection
moulding
process
is
presented.
Inserts
were
fabricated
an
additive
manufacturing
process:
material
jetting
technology.
The
importance
inserts’
orientation
printing
plate
order
samples
more
uniform
thickness
lower
roughness
been
demonstrated
flat
cavity
insert.
addition,
preliminary
tests
carried
out
microstructured
inverted
channels
various
cross-section
shapes
(semi-circular
or
trapezoidal)
widths
(200
300
µm)
investigate
microstructures’
resistance
during
cycles.
best
geometry
was
found
channel
trapezoidal
width
equal
µm.
Finally,
test
performed
demonstrate
devices’
workability.
Biosensors,
Год журнала:
2022,
Номер
12(10), С. 898 - 898
Опубликована: Окт. 20, 2022
The
COVID-19
pandemic
has
severely
impacted
normal
human
life
worldwide.
Due
to
its
rapid
community
spread
and
high
mortality
statistics,
the
development
of
prompt
diagnostic
tests
for
a
massive
number
samples
is
essential.
Currently
used
traditional
methods
are
often
expensive,
time-consuming,
laboratory-based,
unable
handle
large
specimens
in
resource-limited
settings.
Because
contagiousness,
efficient
identification
SARS-CoV-2
carriers
crucial.
As
advantages
adopting
biosensors
diagnosis
increase,
this
narrative
review
summarizes
recent
advances
respective
reasons
consider
applying
biosensors.
Biosensors
most
sensitive,
specific,
rapid,
user-friendly
tools
having
potential
deliver
point-of-care
diagnostics
beyond
standards.
This
provides
brief
introduction
conventional
their
disadvantages.
It
also
discusses
pathogenesis
COVID-19,
biomarkers,
using
biosensor
technology.
current
advancements
biosensing
technologies,
from
academic
research
commercial
achievements,
have
been
emphasized
publications.
We
covered
wide
range
topics,
including
biomarker
detection,
viral
genomes,
proteins,
immune
responses
infection,
other
proinflammatory
biomolecules.
Major
challenges
prospects
future
application
settings
highlighted.
