CRISPR-Microfluidics Nexus: Advancing Biomedical Applications for Understanding and Detection

Sensors and Actuators A Physical, Год журнала: 2024, Номер 376, С. 115625 - 115625

Опубликована: Июнь 24, 2024

The integration of CRISPR technology with microfluidic-based biosensors has greatly expanded its applications in medicine and molecular biology. This combination offers enhanced sensitivity selectivity medical diagnostics. CRISPR-powered microfluidics enables the accurate quantification DNA RNA associated diseases such as cancer, viral infections, bacterial diseases. precise targeting capabilities allow for detection specific genetic sequences, aiding early diagnosis, disease monitoring, treatment assessment. improves specificity by leveraging programmable nature coupling it signal readouts. However, challenges assay optimization, standardization, device fabrication need to be addressed. Additionally, complexity presents potential limitations terms off-target effects unintended modifications. Overall, holds tremendous diagnostics, but further research development are required fully exploit benefits clinical settings.

Язык: Английский

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Terahertz metamaterial biosensor based on open square ring

Advanced Composites and Hybrid Materials, Год журнала: 2023, Номер 6(3)

Опубликована: Май 2, 2023

Язык: Английский

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CRISPR in clinical diagnostics: bridging the gap between research and practice

Bioanalysis, Год журнала: 2025, Номер unknown, С. 1 - 10

Опубликована: Фев. 4, 2025

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has transformed molecular biology through its precise gene-editing capabilities. Beyond initial applications in genetic modification, emerged as a powerful tool diagnostics and biosensing. This review explores transition from genome editing to innovative detection methods, including nucleic acid identification, single nucleotide polymorphism (SNP) analysis, protein sensing. Advanced technologies such SHERLOCK DETECTR demonstrate CRISPR's potential for point-of-care diagnostics, enabling rapid highly sensitive detection. The integration of chemical modifications, CRISPR-Chip technology, enzymatic systems like Cas12a Cas13a enhances signal amplification efficiency. These advancements promise decentralized, real-time diagnostic solutions with significant implications global healthcare. Furthermore, the fusion artificial intelligence digital health platforms is paving way more accessible, cost-effective, scalable approaches, ultimately revolutionizing precision medicine.

Язык: Английский

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Cas-based bacterial detection: recent advances and perspectives

The Analyst, Год журнала: 2024, Номер 149(5), С. 1398 - 1415

Опубликована: Янв. 1, 2024

Rapid and sensitive detection of pathogens. CRISPR/Cas has strong potential application prospects in the field bacterial detection.

Язык: Английский

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Specific and sensitive detection of bovine coronavirus using CRISPR-Cas13a combined with RT-RAA technology

Frontiers in Veterinary Science, Год журнала: 2025, Номер 11

Опубликована: Янв. 7, 2025

Bovine coronavirus (BCoV) is an important pathogen of enteric and respiratory disease in cattle, resulting huge economic losses to the beef dairy industries worldwide. A specific sensitive detection assay for BCoV critical early-stage prevention control. We established a specific, sensitive, stable nucleic acid based on CRISPR/Cas13a combined with reverse transcription recombinase-aided amplification (RT-RAA) technology. The primers RT-RAA CRISPR RNA (crRNA) were designed conserved region nucleocapsid (N) gene. limit assays was 1.72 copies/μl, there no cross-reactions other 10 common bovine disease-associated pathogens. coefficient variations (CVs) within between batches less than 4.98 4.58%, respectively. RT-RAA-CRISPR/Cas13a work well clinical samples cattle yak, positive rate 84 detected by 58.3% (49/84), it notably higher that RT-qPCR (2.4%, 2/84; p < 0.001). 49 further confirmed as Sanger sequencing. developed, providing new technical support epidemiological monitoring BCoV.

Язык: Английский

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Using CRISPR for viral nucleic acid detection

Methods in enzymology on CD-ROM/Methods in enzymology, Год журнала: 2025, Номер unknown, С. 245 - 275

Опубликована: Янв. 1, 2025

Язык: Английский

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CRISPR for Course-Based Undergraduate Research Experiences

Learning materials in biosciences, Год журнала: 2025, Номер unknown, С. 71 - 93

Опубликована: Янв. 1, 2025

Язык: Английский

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CasPro-ESM2: Accurate identification of Cas proteins integrating pre-trained protein language model and multi-scale convolutional neural network

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142309 - 142309

Опубликована: Март 1, 2025

Язык: Английский

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Decoding SPP1 regulation: genetic and non-genetic insights into its role in disease progression

Molecules and Cells, Год журнала: 2025, Номер unknown, С. 100215 - 100215

Опубликована: Апрель 1, 2025

Secreted phosphoprotein 1 (SPP1), also known as osteopontin (OPN), is a multifunctional glycoprotein that plays critical role in various physiological processes, including cell adhesion, chemotaxis, immune regulation, and tissue remodeling. Originally identified key component of the bone matrix, SPP1 now recognized for its broad involvement numerous tissues significant impact on both normal physiology disease progression. Dysregulation has been strongly implicated pathogenesis progression several diseases cancer, cardiovascular diseases, autoimmune disorders, chronic inflammatory conditions. The expression tightly regulated by genetic non-genetic mechanisms. Genetic alterations, such single nucleotide polymorphisms (SNPs), insertions deletions (INDELs), structural variations (SVs) within gene, have associated with increased susceptibility to influencing severity outcomes. Additionally, regulations, DNA methylation, histone modifications, long non-coding RNAs (lncRNAs), play crucial roles modulating response environmental cellular signals. This review provides comprehensive overview regulatory mechanisms governing examines their implications pathogenesis. By integrating recent findings, this highlights complex interplay between predispositions regulations determining activity offers new insights into potential biomarker therapeutic target. Understanding these pathways essential development targeted interventions which pivotal role.

Язык: Английский

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Point-of-Care Testing for Infectious Diseases Based on Class 2 CRISPR/Cas Technology

Diagnostics, Год журнала: 2023, Номер 13(13), С. 2255 - 2255

Опубликована: Июль 3, 2023

The early detection of infectious diseases and microorganisms is critical for effective disease treatment, control, prevention. Currently, nucleic acid testing antigen–antibody serum reaction are the two methods most commonly used diseases. former highly accurate, specific, sensitive, but it time-consuming, expensive, has special technician instrument requirements. latter rapid economical, may not be accurate sensitive enough. Therefore, necessary to develop a quick on-site diagnostic test point-of-care (POCT) enable clinical that convenient, cheap, portable. Here, CRISPR/Cas-based detailed discussed in depth. powerful capacity these will facilitate development tools POCT, though they still have some limitations. This review explores highlights POCT based on class 2 CRISPR/Cas assay, such as Cas12 Cas13 proteins, We also provide an outlook perspectives, multi-application scenarios, applications, limitations technology.

Язык: Английский

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