Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Сен. 14, 2023
Abstract
Background
Non-small
cell
lung
cancer
(NSCLC)
has
long
been
challenged
by
the
complexities
of
chemotherapeutic
resistance,
with
autophagy
playing
a
pivotal
role
in
this
resistance
matrix.
The
multifaceted
interplay
between
and
tumor
behavior,
particularly
context
NSCLC,
demands
nuanced
understanding,
given
its
contradictory
roles
tumorigenesis
survival.
Methods
We
embarked
on
an
intricate
exploration
UDCA's
potential
modulating
MAPK
pathway,
which
is
instigated
DOX.
A
systematic
evaluation
was
undertaken
influence
key
autophagy-associated
proteins
cascading
effects
signaling
pathways,
accentuating
TGFβ
network.
Results
Our
data
illuminated
that
UDCA
exerts
discernible
inhibitory
effect
proliferation
alters
dynamics
intricately
associated
proteins.
Notably,
combined
might
DOX
demonstrated
significant
downregulation
expression,
thereby
achieving
pronounced
retardation
progression
without
amplifying
toxicities.
Yet,
exogenously
introduced
TGF-β
presented
mitigating
counter-effect.
Conclusions
findings
advocate
for
prospective
utility
as
potent
chemosensitizer,
therapeutic
efficacy
against
NSCLC
tactically
inhibiting
autophagy.
This
underscores
imperative
further
exploration,
moving
us
closer
to
tailoring
precision-based
regimens
centered
UDCA’s
unique
modulation
capabilities.
Molecules,
Год журнала:
2024,
Номер
29(6), С. 1195 - 1195
Опубликована: Март 7, 2024
The
species
in
Sanghuangporus
are
a
group
of
edible
mushrooms
with
long
history
oral
use
East
Asia
as
health-improvement
method.
They
should
be
classified
under
the
genus
rather
than
mistakenly
Phellinus
or
Inonotus.
major
components
this
consist
polysaccharides,
polyphenols,
triterpenoids,
and
flavonoids,
all
which
exist
fruiting
bodies
mycelia.
For
extraction,
studies
have
shown
methods
using
hot
water,
ethanol,
DES
solvent,
alkaline,
followed
by
purification
including
traditional
anion
column,
Sevag
solution,
macroporous
resin,
magnetic
polymers.
Proven
modern
medical
technology,
these
possess
promising
anti-inflammatory,
antioxidative,
antitumor,
immunoregulation
effects;
additionally,
they
health-improving
effects
pulmonary
protection,
hypoglycemic
properties,
sleep
improvement,
gout
mitigation,
antiaging,
neuroprotection,
muscle-strengthening
abilities.
Several
toxicity
revealed
their
safety
recommend
dose
1
g/kg
for
mice.
As
newly
emerged
concept,
functional
food
can
provide
not
only
life-sustaining
nutrients
but
also
some
effects.
In
conclusion,
we
substantiate
Sanghuang
comprehensively
presenting
information
on
extraction
methods,
component
structural
nontoxicity,
hoping
to
benefit
development
food.
Biomolecules,
Год журнала:
2024,
Номер
14(6), С. 689 - 689
Опубликована: Июнь 13, 2024
Reactive
oxygen
species
(ROSs)
are
byproducts
of
normal
cellular
metabolism
and
play
pivotal
roles
in
various
physiological
processes.
Disruptions
the
balance
between
ROS
levels
body’s
antioxidant
defenses
can
lead
to
development
numerous
diseases.
Glutathione
peroxidase
3
(GPX3),
a
key
component
system,
is
an
oxidoreductase
enzyme.
GPX3
mitigates
oxidative
damage
by
catalyzing
conversion
hydrogen
peroxide
into
water.
Beyond
its
function,
vital
regulating
metabolism,
modulating
cell
growth,
inducing
apoptosis
facilitating
signal
transduction.
It
also
serves
as
significant
tumor
suppressor
cancers.
Recent
studies
have
revealed
aberrant
expression
several
non-neoplastic
diseases,
associating
it
with
multiple
pathological
This
review
synthesizes
current
understanding
regulation,
highlighting
extensive
noncancerous
Additionally,
this
paper
evaluates
potential
diagnostic
biomarker
explores
emerging
therapeutic
strategies
targeting
enzyme,
offering
avenues
for
future
clinical
treatment
conditions.
Journal of Biochemical and Molecular Toxicology,
Год журнала:
2025,
Номер
39(2)
Опубликована: Янв. 26, 2025
Ischemia-reperfusion
(I/R)
injury
is
a
significant
clinical
problem
impacting
the
heart
and
other
organs,
such
as
kidneys
liver.
This
study
explores
protective
effects
of
oxycodone
on
myocardial
I/R
its
underlying
mechanisms.
Using
model
in
Sprague-Dawley
(SD)
rats
an
oxygen-glucose
deprivation/reoxygenation
(OGD/R)
H9c2
cells,
we
administered
inhibited
AMP-activated
protein
kinase
(AMPK)
with
Compound
C
(C.C).
Our
results
showed
that
significantly
reduced
lactate
dehydrogenase
(LDH)
release
reactive
oxygen
species
(ROS)
production
while
stabilizing
mitochondrial
membrane
potential
(MMP).
Western
blot
RT-qPCR
analyzes
confirmed
enhances
AMPK
phosphorylation
upregulates
expression
Silent
Information
Regulator
1
(SIRT1)
Peroxisome
Proliferator-Activated
Receptor
γ
Coactivator
1α
(PGC-1α),
thereby
protecting
cells.
These
findings
suggest
exerts
against
by
activating
pathway,
offering
new
therapeutic
targets
for
protection.
Journal of Cachexia Sarcopenia and Muscle,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 28, 2025
ABSTRACT
Background
Muscle
atrophy
is
associated
with
Type
2
diabetes
mellitus,
which
reduces
the
quality
of
life
and
lacks
effective
treatment
strategies.
Previously,
it
was
determined
that
human
umbilical
cord
mesenchymal
stromal
cell
(hucMSC)–derived
exosomes
(EXOs)
ameliorate
diabetes‐induced
muscle
atrophy.
However,
systemic
application
EXOs
less
selective
for
diseased
tissues,
their
efficacy
safety
nonspecific
biological
distribution
in
vivo.
Therefore,
improving
exosomal
targeting
imperative.
In
this
study,
a
skeletal
muscle–specific
aptamer
(Apt)
used
to
explore
effects
Apt‐functionalized
derived
from
hucMSCs
diabetes‐associated
its
specific
mechanisms.
Methods
Diabetic
db/db
mice
C2C12
myotubes
were
MSC‐EXOs
or
Apt‐EXOs
alleviating
Grip
strength,
weight
fibre
cross‐sectional
area
(CSA)
evaluate
strength
mass.
Western
blot
analysis
signalling,
including
MuRF1
Atrogin
1
mitochondrial
complex
Seahorse
performed
investigate
underlying
mechanisms
on
Results
increased
grip
(
p
=
0.0002)
mass
0.0044
tibialis
anterior
(TA)
muscle,
0.002
soleus
(SO)
muscle)
mice.
It
also
CSA
fibres
0.0011
all
fibres,
0.0036
slow
0.0089
fast
fibres)
percentage
slow‐to‐fast
0.0109).
0.0455)
expression
0.0168)
reduced.
activated
SIRT1/FoxO1/3a
signalling
enhanced
function
myotubes.
SIRT1
knockdown
decreased
beneficial
antiatrophic
MSC‐EXOs.
Additionally,
Apt
conjugation
effect
myofiber‐type
transition
0.0133
0.0124
TA
weight,
0.0008
SO
<
0.0001
0.0198
0.0213
0.011
slow–fast
0.0141
0.005
expression).
Conclusions
The
results
suggest
hucMSC‐derived
by
enhancing
SIRT1/FoxO1/3a‐mediated
strengthens
These
findings
demonstrate
therapeutic
potential
muscle‐targeted
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(7), С. 3444 - 3444
Опубликована: Апрель 7, 2025
Sanghuang
(Sanghuangporus
sanghuang,
SS)
is
a
medicinal
fungus
with
multiple
pharmacological
effects,
including
antioxidant,
anti-inflammatory,
immune-boosting,
and
anti-cancer
activities.
5-fluorouracil
(5-FU)
commonly
used
chemotherapeutic
agent
for
the
treatment
of
colorectal
cancer.
It
primarily
exerts
its
antitumor
effect
by
inhibiting
DNA
RNA
synthesis,
leading
to
cell
apoptosis.
However,
it
frequently
induces
adverse
effects
These
issues
limit
clinical
application
5-FU.
This
research
aims
determine
potential
SS
as
therapeutic
in
reducing
5-FU-induced
intestinal
mucositis
mouse
model.
The
results
indicated
that
5-FU
administration
significantly
increased
diarrhea
severity,
reduced
colon
length,
caused
small
villus
atrophy,
disrupted
architecture,
led
insufficient
crypt
proliferation,
resulted
weight
loss.
also
upregulated
inflammatory
responses,
apoptosis,
oxidative
stress,
epithelial-mesenchymal
transition
(EMT)
pathways,
integrity
mucosal
tight
junction,
while
elevating
pro-inflammatory
cytokines
antioxidant
capacity.
ameliorating
alleviating
impacts
on
mucosa.
In
conclusion,
this
investigation
provides
first
evidence
protective
mucositis.
findings
suggest
application,
offering
promising
strategy
chemotherapy
improving
quality
life
cancer
patients.
Drug and Chemical Toxicology,
Год журнала:
2025,
Номер
unknown, С. 1 - 10
Опубликована: Апрель 14, 2025
Ginkgo
biloba
extract
(GBE)
has
demonstrated
therapeutic
potential
in
paraquat
(PQ)-induced
injury;
however,
its
molecular
mechanism
remains
unclear.
The
cell
viability,
lactate
dehydrogenase
(LDH)
release,
and
apoptosis
of
RLE-6TN
cells
were
assessed
using
CCK-8,
LDH
assay,
flow
cytometry.
Oxidative
stress
was
evaluated
the
DCFH-DA
probe,
while
inflammatory
cytokine
levels
(TNF-α,
IL-1β,
IL-6)
measured
via
ELISA.
expression
miR-155-5p
analyzed
qRT-PCR.
Bioinformatics
analysis,
dual-luciferase
reporter
RNA
immunoprecipitation
(RIP)
assay
employed
to
investigate
interaction
between
SIRT1
miR-155-5p.
Protein
SIRT1,
TLR4,
phosphorylated
P65
(p-P65),
total
determined
by
western
blotting.
results
showed
that
upregulated
PQ-induced
injury.
Knockdown
improved
viability
reduced
apoptosis,
oxidative
stress,
damage
PQ-treated
cells.
In
cells,
directly
targeted
SIRT1/TLR4
axis.
GBE
mitigated
injury
modulating
miR-155-5p/SIRT1/TLR4
axis,
thereby
enhancing
reducing
inflammation.
This
study
indicates
GEB
may
protect
against
RLE-cell
regulating
signaling
pathway
could
be
a
promising
biomarker
for
PQ
intoxication,
providing
novel
insights
into
strategies
poisoning.
