A novel tissue-engineered corneal epithelium based on ultra-thin amniotic membrane and mesenchymal stem cells

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июль 29, 2024

Currently, in vitro cultured corneal epithelial transplantation is effective treating limbal stem cell dysfunction (LSCD). Selecting carriers crucial for constructing the epithelium through tissue engineering. In this study, traditional amniotic membrane (AM) was modified, and mesenchymal cells (MSCs) were inoculated into ultra-thin (UAM) stroma to construct a novel UAM-MSC tissue-engineered carrier, that could effectively simulate (LSCs) microenvironment. The structure of different managed rabbit LSCD model corneas observed hematoxylin–eosin staining. Cell phenotypes evaluated fluorescence staining, Western blotting, RT-qPCR. Additionally, junction genes expression markers related anti-neovascularization using Corneal junctions via an electron microscope. culture medium analyzed mass spectrometry. Tissue-engineered expanded by LSCs on UAM-MSCs had good transparency. Simultaneously, progenitor (K14, PNCA, p63) (PAX6) gene constructed higher than amplified UAM de-epithelialized membrane. Electron microscopy revealed grafted with closely connected. conclusion, vector we better microenvironment; transparency, properties, closer intercellular connections, resemblance natural phenotype.

Язык: Английский

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Tauroursodeoxycholic acid regulates macrophage/monocyte distribution and improves spinal microenvironment to promote nerve regeneration through inhibiting NF-κB signaling pathway in spinal cord injury

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Апрель 10, 2025

Following spinal cord injury (SCI), blood-borne monocytes infiltrate the cord, differentiate into macrophages, and dominate lesion site. Inflammatory responses mediated by macrophages determine nerve regeneration functional recovery after SCI. Tauroursodeoxycholic acid (TUDCA) shows a neuroprotective effect in different SCI animal models. However, underlying mechanism of TUDCA regulating monocytes/macrophages to impact has not been elucidated clearly. This study aims investigate on monocyte/macrophage distribution subacute stage Transwell analysis, Bromodeoxyuridine (BrdU) staining, TUNEL staining were performed evaluate inflammatory response neural stem cells (NSCs) proliferation migration, neuron survival, axon degeneration vitro. H&E RNA sequencing, series immunofluorescent pathological progress, gene expression changes, distribution, treatment mice. We found restored NSCs migration reduced neurons apoptosis promoted wound healing, down-regulated genes related response, up-regulated development Our provided evidence that regulated improved microenvironment promote

Язык: Английский

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Limosilactobacillus reuteri DSM17938 Attenuates Neuroinflammatory Responses After Spinal Cord Injury by Modulating Tryptophan Metabolism

Probiotics and Antimicrobial Proteins, Год журнала: 2025, Номер unknown

Опубликована: Апрель 26, 2025

Spinal cord injury (SCI) disrupts gut flora and exacerbates neuroinflammation. Evidence supports the important role of intestinal microbiota in SCI. This study evaluated neuroprotective effect Limosilactobacillus reuteri (L. reuteri) DSM 17938 on SCI its potential anti-inflammatory mechanism. The was disorganised following SCI, with a significant decrease abundance probiotic bacteria such as L. reuteri. DSM17938 treatment improved spinal pathology enhanced locomotor functional recovery SCI-model rats. Moreover, it modulated tryptophan metabolism by promoting indole-3-carboxaldehyde production. In addition, inhibits polarization M1 microglia reduces production IL-6, IL-1 β, TNF-α to alleviate It also activates aryl hydrocarbon receptor (AhR) signalling via upregulating AhR CYP1A1 expression, tight junction protein synthesis. summary, promotes modulating activate barrier repair attenuate microglial activation neuroinflammation, suggesting strategy for clinical adjuvant treatment.

Язык: Английский

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Regenerative Inflammation: The Mechanism Explained from the Perspective of Buffy-Coat Protagonism and Macrophage Polarization

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(20), С. 11329 - 11329

Опубликована: Окт. 21, 2024

The buffy-coat, a layer of leukocytes and platelets obtained from peripheral blood centrifugation, plays crucial role in tissue regeneration the modulation inflammatory responses. This article explores mechanisms regenerative inflammation, highlighting critical buffy-coat influencing macrophage polarization its therapeutic potential. Macrophage into M1 M2 subtypes is pivotal balancing inflammation repair, with macrophages driving pro-inflammatory responses promoting healing regeneration. buffy-coat's rich composition progenitor cells, cytokines, growth factors-such as interleukin-10, transforming factor-β, monocyte colony-stimulating factor-supports transition to macrophages, enhancing repair resolution inflammation. dynamic interaction between components opens new avenues for strategies aimed at improving managing conditions, particularly musculoskeletal diseases such osteoarthritis. Furthermore, use buffy-coat-derived therapies conjunction other modalities, platelet-rich plasma, holds promise more effective clinical outcomes.

Язык: Английский

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PBMC-mediated modulation of macrophage polarization in RAW264.7 cells through STAT1/STAT6 signaling cascades

International Immunopharmacology, Год журнала: 2024, Номер 138, С. 112651 - 112651

Опубликована: Июль 9, 2024

Peripheral blood mononuclear cells (PBMC), sourced autologously, offer numerous advantages when procured: easier acquisition process, no in vitro amplification needed, decreased intervention and overall increased acceptability make PBMC an attractive candidate for cell therapy treatment. However, the exact mechanism by which treat diseases remains poorly understood. Immune imbalance is pathological basis of many diseases, with macrophages playing a crucial role this process. research on mechanisms regulating scarce. This study employed co-culture model RAW264.7 to explore macrophages. The results showed that co-culturing led expression inflammatory cytokines anti-inflammatory or culture supernatant. Additionally, pro-inflammatory, tissue matrix-degrading M1 decreased, while anti-inflammatory, matrix-synthesizing, regenerative M2 both monocytes within PBMC. Moreover, co-cultured exhibited significantly p-STAT1/STAT1 ratio, p-STAT6/STAT6 ratio increased. suggests may inhibit macrophage polarization blocking STAT1 signaling cascades promote through activation STAT6 cascades. Overall, sheds light it was found differentiated into presence finding provides experimental evidence use treating especially macrophage-depleting such as osteoarthritis.

Язык: Английский

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IL-11 Plays a Protective Anti-Inflammatory Role in Spinal Cord Injury by Inhibiting Microglial Activation Through Suppression of ISG15 Signaling

Опубликована: Янв. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Язык: Английский

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A novel tissue-engineered corneal epithelium based on ultra-thin amniotic membrane and mesenchymal stem cells

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Апрель 12, 2024

Abstract Purpose: Currently, in vitro cultured corneal epithelial transplantation is effective for treating limbal stem cell dysfunction (LSCD). Selecting carriers crucial constructing the epithelium through tissue engineering. This study aimed to effectively simulate cells (LSCs) microenvironment by inoculating mesenchymal (MSCs) on basal surface of an ultra-thin amniotic membrane (UAM) develop improved tissue-engineered carrier. Methods: Traditional AM was modified, and MSCs were inoculated into UAM stroma construct a novel UAM-MSC The structure rabbit LSCD model corneas different observed hematoxylin eosin staining. Cell phenotypes evaluated fluorescence staining, WB, RT-qPCR. Additionally, junction genes expression markers related anti-neovascularization using Corneal junctions via electron microscope. culture medium analyzed mass spectrometry. Results: Tissue-engineered expanded LSCs UAM-MSCs had good transparency. Simultaneously, progenitor (K14, PNCA, p63) (PAX6) gene constructed higher than that amplified de-epithelialized membrane. Electron microscopy revealed grafted with closely connected. Conclusions: vector we can better microenvironment, transparency, properties, closer intercellular connections, resemblance natural phenotype.

Язык: Английский

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Modern cell culture technologies: Revolutionizing neuroregeneration in neuropsychiatry

Archives of Biological Psychiatry, Год журнала: 2024, Номер 2, С. 14 - 24

Опубликована: Июль 6, 2024

This review highlights the latest developments in current cell culture methods, including three-dimensional culture, organoids, coculture systems, microfluidics, and nanofiber scaffolds to support neuroregeneration major neuropsychiatric illnesses. Due enhanced vitro modeling of human brain structure function, these state-of-the-art methods allow for investigations disease processes drug screening, pathophysiological research on has increased. We examine recent relationship between technologies conditions such as stroke, Alzheimer’s, traumatic injury, spinal cord injury. The advancements present encouraging prospects augmenting could facilitate stem cell-based therapies ailments that were previously untreatable.

Язык: Английский

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Prognostic Value of Magnetic Resonance Imaging Variables Combined with Neutrophil-to-Lymphocyte Ratio in Patients with Cervical Traumatic Spinal Cord Injury

World Neurosurgery, Год журнала: 2024, Номер 190, С. e684 - e693

Опубликована: Авг. 5, 2024

Язык: Английский

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Macrophages Modulate Optic Nerve Crush Injury Scar Formation and Retinal Ganglion Cell Function

Investigative Ophthalmology & Visual Science, Год журнала: 2024, Номер 65(10), С. 22 - 22

Опубликована: Авг. 14, 2024

Purpose: Optic nerve (ON) injuries can result in vision loss via structural damage and cellular injury responses. Understanding the immune response, particularly role of macrophages, response to ON is crucial for developing therapeutic approaches which affect repair. The present study investigates macrophages fibrotic scar formation, retinal ganglion cell (RGC) function. Methods: utilizes macrophage Fas-induced apoptosis (MaFIA) mice selectively deplete hematogenous explores impact have on Histological immunofluorescence analyses were used evaluate expression levels formation. Pattern electroretinogram (PERG) recordings assess RGC function as injury. Results: Successful depletion was induced MaFIA mice, led reduced formation post-injury. Despite an increase activated retina, preserved, demonstrated by normal PERG waveforms up 2 months suggests a neuroprotective repair highlights complex Conclusions: To our knowledge, this first use demonstrate that targeted leads significant reduction size preservation functionality after These findings highlight key opens new avenues interventions injuries. Future research should focus investigating distinct roles subtypes potential macrophage-associated molecular targets improve regeneration

Язык: Английский

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