Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1)
Опубликована: Апрель 10, 2024
A "redox-stat" R
Язык: Английский
Environmental Science & Technology, Год журнала: 2023, Номер 58(1), С. 683 - 694
Опубликована: Дек. 15, 2023
The coculture theory that promotes denitrification relies on effectively utilizing the resources of low-efficiency microbes. Here, strains Streptomyces sp. PYX97 and TSJ96 were isolated showed lower capacity when cultured individually. However, enhanced nitrogen removal (removed 96.40% total nitrogen) organic carbon reduction 92.13% dissolved carbon) under aerobic conditions. Nitrogen balance analysis indicated coculturing efficiency nitrate converted into gaseous reaching 70.42%. Meanwhile, promoted cell metabolism source metabolic activity. thrived in conditions C/N = 10, alkalescence, 150 rpm shaking speed. reduced CODMn raw water treatment by 83.32 84.21%, respectively. During this treatment, activity density increased reactor. Moreover, could utilize aromatic protein soluble microbial products during processes treatment. This study suggests inefficient actinomycete be a promising approach for treating polluted bodies.
Язык: Английский
Процитировано
11
Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 279, С. 116385 - 116385
Опубликована: Май 20, 2024
Heterotrophic nitrifying bacteria are found to be promising candidates for implementation in wastewater treatment systems due their tolerance extreme environments. A novel acid-resistant bacterium, Pseudomonas citronellolis YN-21, was isolated and reported have exceptional heterotrophic nitrification capabilities acidic condition. At pH 5, the highest NH
Язык: Английский
Процитировано
4
Опубликована: Янв. 1, 2025
The roles of napA and narG genes encoding nitrate reductase in the nitrogen cycle remain unclear. In this study, we constructed napA- narG-knockout mutants complementary strains compared their transformation characteristics with those wild-type strain Pseudomonas putida Y-9, which can simultaneously perform assimilation (ANRA), dissimilatory reduction to ammonium (DNRA), denitrification under aerobic conditions. deletion did not affect removal (NO3-) by P. efficiency mutant (88.59%) was comparable that (87.48%). However, severely inhibited growth NO3- Y-9. A 15N isotope experiment demonstrated controls DNRA ANRA pathways Knocking out significantly nitrite (NO2-) NO2- narG-deficient (82.30 83.81%, respectively) lower than (97.00%). Although these two completely removed (NH4+), less NH4+ converted into gaseous (11.96 13.98%, via oxidation (22.89%). Meanwhile, glnA, regulates assimilation, upregulated. Our results clarified narG, rather napA, mediates ANRA, DNRA, Y-9 Furthermore, both conversion NH4+.
Язык: Английский
Процитировано
0
Bioresource Technology, Год журнала: 2025, Номер unknown, С. 132100 - 132100
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Environmental Science & Technology Letters, Год журнала: 2025, Номер unknown
Опубликована: Фев. 5, 2025
Язык: Английский
Процитировано
0
Water, Год журнала: 2025, Номер 17(4), С. 502 - 502
Опубликована: Фев. 11, 2025
Managing nitrogen and phosphorus pollution in high-salinity wastewater is a critical challenge for sustainable aquaculture environmental protection. In this study, novel salt-tolerant strain, Pseudomonas sediminis D4, was isolated from brackish water environment. This strain exhibited simultaneous heterotrophic nitrification–aerobic denitrification removal capabilities. Biosafety assays demonstrated that the antibiotic-sensitive safe aquatic environments. The optimal conditions phosphate of D4 were carbon/nitrogen (C/N) ratio 10, phosphorus/nitrogen (P/N) 0.2, pH 7, temperature 30 °C while using sodium succinate as carbon source. Under these conditions, achieved efficiencies 97.36% ammonia (NH4+-N), 100.00% nitrate (NO3−-N), 98.02% nitrite (NO2−-N), along with 94.69%, 89.56%, 97.40% PO43−P, respectively. strong salinity tolerance, functioning effectively within range 0% to 5% (w/v), maintaining high efficiency at 3%. Enzyme activity verified existence key enzymes, such oxidase, oxidoreductase, reductase, polyphosphate kinase, exopolyphosphatase, which are essential nitrification-aerobic capabilities D4. These findings highlight potential biological treatment wastewater.
Язык: Английский
Процитировано
0
Environmental Microbiology, Год журнала: 2025, Номер 27(3)
Опубликована: Март 1, 2025
ABSTRACT The growth and nitrogen metabolism of heterotrophic nitrification‐aerobic denitrification (HN‐AD) bacteria are affected by nitrite, but the mechanisms underlying this for strain Acinetobacter johnsonii EN‐J1 unclear. In study, addition 10 mg/L nitrite increased reduction rate ammonium 1.0 mg/L/h, 20 nitrate 3.9 mg/L/h. Compared with control, reductase activity, electron transfer adenosine triphosphate content were enhanced 142.0%, 278.0% 279.0%, respectively, in removal process after nitrite. whole genome was annotated genes such as narGHI , narK nsrR nirBD nasA glnA gltB gdhA amt . Transcriptome analysis showed that triggered significant upregulation several key pathways, including metabolism, tricarboxylic acid cycle, amino enhancing denitrification. expression ( narG hmp ) detected real‐time quantitative polymerase chain reaction. These results suggested enhances increasing genes, levels, which is important elucidating mechanism promotion biological efficiency.
Язык: Английский
Процитировано
0
The Science of The Total Environment, Год журнала: 2022, Номер 858, С. 159628 - 159628
Опубликована: Окт. 23, 2022
Язык: Английский
Процитировано
17
Bioresource Technology, Год журнала: 2024, Номер 414, С. 131574 - 131574
Опубликована: Окт. 7, 2024
Язык: Английский
Процитировано
3
Bioresource Technology, Год журнала: 2022, Номер 365, С. 128156 - 128156
Опубликована: Окт. 19, 2022
Язык: Английский
Процитировано
15