Biological Diversity and Conservation, Год журнала: 2023, Номер unknown

Опубликована: Дек. 27, 2023

Isolation and Characterization of Microorganisms for Use with Manure Chemical Fertilizers Levent Değirmenci1, Özge Kaygusuz İzgördü2, Cihan Darcan3 ORCID: 0000-0001-6608-0398; 0000-0002-3652-4266; 0000-0003-0205-3774 1 Corresponding author: Department Engineering, Bilecik Şeyh Edebali University, 11210, Bilecik/Turkey Tel: +90 228 2141548, e-mail: [email protected] 2 Biotechnology Application Research Center, Bilecik, Turkey 3 3Department Molecular Biology Genetics, Turkey Abstract A microbial consortium the ability to utilize nitrogen certain chemical fertilizers were established via a series tests. Results indicated four strains, which identified as Bacillus cereus sp an Alcaligenes faecalis sp. was only non-Bacillus member consortium. The members showed no antagonistic activity against each other implying their successful utilization components commercial fertilizer. Keywords: IAA, siderophore, spp., fixation, ammonia/nitrite oxidation, MALDI-TOF MS Organik ve Kimyasal Gübre ,le Kullanılmak Üzere Mikroorganizma İzolasyon Karakterizasyonu Özet Farklı kimyasal gübrelerin içeriğinde yer alan azotu bitkinin kullanımına sunabilen mikroorganizma karışımı bir dizi testler sonucunda elde edilmiştir. Sonuçların değerlendirilmesiyle karışımına üç Cereus suşu ile karışımın olmayan tek üyesi seçilmiştir. karışımında suşların birbirlerine karşı antagonistik etki göstermediği dolayısıyla mikrobiyal gübre olarak ticarileştirilmesinin mümkün olduğu da edilen sonuçlar arasındadır. Anahtar Kelimeler: spp. azot fiksasyonu, amonyum/nitrit oksidasyonu, 1. Introduction fertilizer is gaining increasing attention its harmful effects on environment possesses serious threat [2]. Heavy metal accumulation another problem during [2].The variation soil pH, encountered due excessive utilization, has negative impact phosphorus uptake [3]. Plant growth promoting bacteria when added soil, operate mechanisms facilitating acquisition already existing resources in [4]. Siderophore producing also acts biocontrol agent capable decreasing phytopathogen population flora [4–7]. Certain procedures developed and/or modified present study obtain would act enhancer plant growth. 2. Materials Method 2.1. Semi-selective isolation procedure starting-point conducted according [12]. 2.2. Standard tests applied determine potential isolates PGPB. Nitrogen fixation ability, IAA production, phosphate solubility siderophore production [13-18]. 2.3. Specified evaluation PGP isolates determined [19]. 2.4. Biochemical accordance MS analyses evaluated validation. based positive results both caseinase starch hydrolysis [20-22]. selected strains isolate towards production. 3. performance, Ammonifier selection, solubility, urea degradation, ammonium oxidation nitrite abilities establish 4. Conclusion discussion • Media selection could serve criterion assemble group microorganisms. Tests be diversified properties. Test facilitate establishment consortium. Strains different properties case our study. Identification species level possible extent. analysis presented brief idea similar have been obtained applying costlier alternatives. Hence relatively simpler concluded better initial investigation. Evaluation led investigation prospects including determination physicotolerant management Fusarium wilt disease topics future investigations.

Язык: Английский