ACS Synthetic Biology,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 15, 2025
Caffeic
acid
(CA)
and
chlorogenic
(CGA)
have
diverse
health
benefits,
including
hemostatic,
antioxidant,
antiinflammatory,
highlighting
their
potential
for
medical
applications.
However,
the
absence
of
high-performance
production
strains
increases
costs,
limiting
wider
application.
In
this
study,
we
engineered
Escherichia
coli
de
novo
CA
CGA.
To
improve
production,
a
highly
efficient
mutant
tyrosine
ammonia-lyase
from
Rhodotorula
taiwanensis
(RtTALT415M/Y458F)
was
identified
using
genome
mining
protein
engineering.
By
engineering
biosynthetic
pathway
through
deletion
pheA
tyrR,
along
with
overexpression
aroGfbr
tyrAfbr,
developed
an
E.
strain,
CA11,
which
produced
6.36
g/L
yield
0.06
g/g
glucose
productivity
0.18
g/L/h.
This
represents
highest
titer
reported
microbial
synthesis
as
sole
carbon
source
in
coli.
Based
on
strain
further
CGA13,
optimized
replicons,
promoters,
ribosome-binding
sites,
1.53
CGA
fed-batch
fermentation,
its
industrial-scale
production.
Molecules,
Год журнала:
2023,
Номер
28(4), С. 1845 - 1845
Опубликована: Фев. 15, 2023
Phenolic
compounds
and
flavonoids
are
potential
substitutes
for
bioactive
agents
in
pharmaceutical
medicinal
sections
to
promote
human
health
prevent
cure
different
diseases.
The
most
common
found
nature
anthocyanins,
flavones,
flavanones,
flavonols,
flavanonols,
isoflavones,
other
sub-classes.
impacts
of
plant
phenolics
on
promoting
diseases
curing
preventing
antioxidant
effects,
antibacterial
impacts,
cardioprotective
anticancer
immune
system
promoting,
anti-inflammatory
skin
protective
effects
from
UV
radiation.
This
work
aims
provide
an
overview
phenolic
as
important
sources
medical
application
according
recently
published
studies,
well
some
interesting
directions
future
research.
keyword
searches
flavonoids,
phenolics,
tannins,
coumarins,
lignans,
quinones,
xanthones,
curcuminoids,
stilbenes,
cucurmin,
phenylethanoids,
secoiridoids
were
performed
by
using
Web
Science,
Scopus,
Google
scholar,
PubMed.
acids
contain
a
carboxylic
acid
group
addition
the
basic
structure
mainly
divided
into
hydroxybenzoic
hydroxycinnamic
acids.
Hydroxybenzoic
based
C6-C1
skeleton
often
bound
small
organic
acids,
glycosyl
moieties,
or
cell
structural
components.
Common
include
gallic,
syringic,
protocatechuic,
p-hydroxybenzoic,
vanillic,
gentistic,
salicylic
Hydroxycinnamic
C6-C3
also
molecules
such
quinic
glucose.
main
caffeic,
p-coumaric,
ferulic,
sinapic
World Journal of Microbiology and Biotechnology,
Год журнала:
2024,
Номер
40(2)
Опубликована: Янв. 16, 2024
Abstract
Because
of
the
hydrophobic
nature
membrane
lipid
bilayer,
majority
hydrophilic
solutes
require
special
transportation
mechanisms
for
passing
through
cell
membrane.
Integral
transport
proteins
(MTPs),
which
belong
to
Major
Intrinsic
Protein
Family,
facilitate
these
across
membranes.
MTPs
including
aquaporins
and
carrier
are
transmembrane
spanning
The
easy
handling
microorganisms
enabled
discovery
a
remarkable
number
specific
different
substances.
It
has
been
realized
that
transporters
have
very
important
roles
in
survival
microorganisms,
their
pathogenesis,
antimicrobial
resistance.
Astonishing
features
related
solute
specificity
led
acceleration
research
on
properties
development
innovative
products
unique
used
or
imitated.
Studies
microbial
range
from
characterization
novel
transporter
protein
mining
screening
them
large
library
particular
functions,
simulations
modeling
preparation
biomimetic
synthetic
materials
purposes
such
as
biosensors
filtration
This
review
presents
recent
discoveries
focuses
especially
formate
nitrite
aquaporins,
advances
biotechnological
applications.
Food Chemistry X,
Год журнала:
2024,
Номер
23, С. 101776 - 101776
Опубликована: Авг. 27, 2024
Fruits
contain
numerous
polyphenols
in
the
form
of
conjugates,
which
exhibit
low
antioxidant
activity.
Probiotic
fermentation
is
a
strategy
to
improve
activity
these
conjugated
by
modifying
their
structure.
However,
mechanisms
underlying
effects
functional
groups
and
derivatizations
on
antioxidative
activities
antioxidation
enhancement
probiotic
biotransformation
haven't
been
comprehensively
explored.
This
review
aimed
explore
structure-antioxidant
relationships
four
three
flavonoids
phenolic
acids.
Further,
elucidated
acids
as
glycoside,
methylated,
ester
conjugates
biotransformation.
Deglycosylation,
demethylation,
hydrolysis
catalyzed
enzymes
produced
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 2, 2025
Chlorogenic
acid
(CGA)
is
a
natural
hydroxycinnamic
ester
with
significant
applications
in
food
preservation
and
nutritional
health.
However,
extraction
of
CGA
from
plants
challenging,
resulting
low
purity
that
fails
to
meet
increasing
market
demands.
Furthermore,
the
broad
substrate
specificity
hydroxycinnamoyl-CoA:quinic
transferase
catalysis
generating
plethora
byproducts,
lack
NADPH
regeneration,
presence
degrading
proteins
impede
microbial
synthesis
CGA.
This
study
achieved
de
novo
Yarrowia
lipolytica
by
introducing
hydroxylation
condensation
modules
based
on
screening
synthetic
pathway
genes
optimizing
parallel
promoters.
Additionally,
an
regeneration
system
was
incorporated
enhance
efficiency
hydroxylation,
thereby
titer
333.16
mg/L.
From
transcriptome
data,
528
significantly
upregulated
were
identified,
deletion
YALI0_B21824g
slowed
rate
degradation,
which
increased
351.33
mg/L
shake
flasks.
Applying
fed-batch
fermentation
5
L
bioreactor
further
production
4837.32
(64
mg/g
DCW).
established
Y.
lipolytica,
providing
foundation
for
coumaric
its
derivatives.
