De novo Biosynthesis of Caffeic Acid and Chlorogenic Acid in Escherichia coli via Enzyme Engineering and Pathway Engineering DOI
Zhenyu Zhang,

Pengfu Liu,

Bin Zhang

и другие.

ACS Synthetic Biology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 15, 2025

Caffeic acid (CA) and chlorogenic (CGA) have diverse health benefits, including hemostatic, antioxidant, antiinflammatory, highlighting their potential for medical applications. However, the absence of high-performance production strains increases costs, limiting wider application. In this study, we engineered Escherichia coli de novo CA CGA. To improve production, a highly efficient mutant tyrosine ammonia-lyase from Rhodotorula taiwanensis (RtTALT415M/Y458F) was identified using genome mining protein engineering. By engineering biosynthetic pathway through deletion pheA tyrR, along with overexpression aroGfbr tyrAfbr, developed an E. strain, CA11, which produced 6.36 g/L yield 0.06 g/g glucose productivity 0.18 g/L/h. This represents highest titer reported microbial synthesis as sole carbon source in coli. Based on strain further CGA13, optimized replicons, promoters, ribosome-binding sites, 1.53 CGA fed-batch fermentation, its industrial-scale production.

Язык: Английский

Therapeutic Potential of Phenolic Compounds in Medicinal Plants—Natural Health Products for Human Health DOI Creative Commons
Wenli Sun, Mohamad Hesam Shahrajabian

Molecules, Год журнала: 2023, Номер 28(4), С. 1845 - 1845

Опубликована: Фев. 15, 2023

Phenolic compounds and flavonoids are potential substitutes for bioactive agents in pharmaceutical medicinal sections to promote human health prevent cure different diseases. The most common found nature anthocyanins, flavones, flavanones, flavonols, flavanonols, isoflavones, other sub-classes. impacts of plant phenolics on promoting diseases curing preventing antioxidant effects, antibacterial impacts, cardioprotective anticancer immune system promoting, anti-inflammatory skin protective effects from UV radiation. This work aims provide an overview phenolic as important sources medical application according recently published studies, well some interesting directions future research. keyword searches flavonoids, phenolics, tannins, coumarins, lignans, quinones, xanthones, curcuminoids, stilbenes, cucurmin, phenylethanoids, secoiridoids were performed by using Web Science, Scopus, Google scholar, PubMed. acids contain a carboxylic acid group addition the basic structure mainly divided into hydroxybenzoic hydroxycinnamic acids. Hydroxybenzoic based C6-C1 skeleton often bound small organic acids, glycosyl moieties, or cell structural components. Common include gallic, syringic, protocatechuic, p-hydroxybenzoic, vanillic, gentistic, salicylic Hydroxycinnamic C6-C3 also molecules such quinic glucose. main caffeic, p-coumaric, ferulic, sinapic

Язык: Английский

Процитировано

350

Coordinating caffeic acid and salvianic acid A pathways for efficient production of rosmarinic acid in Escherichia coli DOI
Lian Wang, Huijing Wang, Jianbin Chen

и другие.

Metabolic Engineering, Год журнала: 2023, Номер 76, С. 29 - 38

Опубликована: Янв. 6, 2023

Язык: Английский

Процитировано

29

Microbial membrane transport proteins and their biotechnological applications DOI Creative Commons
Melek Özkan, Hilal Yılmaz, Pınar Ergenekon

и другие.

World Journal of Microbiology and Biotechnology, Год журнала: 2024, Номер 40(2)

Опубликована: Янв. 16, 2024

Abstract Because of the hydrophobic nature membrane lipid bilayer, majority hydrophilic solutes require special transportation mechanisms for passing through cell membrane. Integral transport proteins (MTPs), which belong to Major Intrinsic Protein Family, facilitate these across membranes. MTPs including aquaporins and carrier are transmembrane spanning The easy handling microorganisms enabled discovery a remarkable number specific different substances. It has been realized that transporters have very important roles in survival microorganisms, their pathogenesis, antimicrobial resistance. Astonishing features related solute specificity led acceleration research on properties development innovative products unique used or imitated. Studies microbial range from characterization novel transporter protein mining screening them large library particular functions, simulations modeling preparation biomimetic synthetic materials purposes such as biosensors filtration This review presents recent discoveries focuses especially formate nitrite aquaporins, advances biotechnological applications.

Язык: Английский

Процитировано

16

Development of a vitamin B5 hyperproducer in Escherichia coli by multiple metabolic engineering DOI
Fuqiang Song,

Zhijie Qin,

Kun Qiu

и другие.

Metabolic Engineering, Год журнала: 2024, Номер 84, С. 158 - 168

Опубликована: Июнь 26, 2024

Язык: Английский

Процитировано

12

Effects of derivatization and probiotic transformation on the antioxidative activity of fruit polyphenols DOI Creative Commons
Yixuan Wang, Chenxi Wang, Junling Shi

и другие.

Food Chemistry X, Год журнала: 2024, Номер 23, С. 101776 - 101776

Опубликована: Авг. 27, 2024

Fruits contain numerous polyphenols in the form of conjugates, which exhibit low antioxidant activity. Probiotic fermentation is a strategy to improve activity these conjugated by modifying their structure. However, mechanisms underlying effects functional groups and derivatizations on antioxidative activities antioxidation enhancement probiotic biotransformation haven't been comprehensively explored. This review aimed explore structure-antioxidant relationships four three flavonoids phenolic acids. Further, elucidated acids as glycoside, methylated, ester conjugates biotransformation. Deglycosylation, demethylation, hydrolysis catalyzed enzymes produced

Язык: Английский

Процитировано

10

Modular cascade with engineered HpaB for efficient synthesis of hydroxytyrosol DOI
Wenkai Liu,

Xiu‐Xin Ren,

Lian Xu

и другие.

Bioorganic Chemistry, Год журнала: 2025, Номер 155, С. 108125 - 108125

Опубликована: Янв. 4, 2025

Язык: Английский

Процитировано

1

Ethylene Glycol Conversion to Glycolic Acid in Escherichia coli: Leveraging Transcriptomic Data for Enhanced Productivity DOI

Y.S. Gong,

Wenlong Yan,

Fei Liu

и другие.

Journal of environmental chemical engineering, Год журнала: 2025, Номер unknown, С. 115326 - 115326

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1

De Novo Biosynthesis of Chlorogenic Acid in Yarrowia lipolytica through Cis-Acting Element Optimization and NADPH Regeneration Engineering DOI

Wenjing He,

Mengsu Liu,

Mingyu Yue

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 2, 2025

Chlorogenic acid (CGA) is a natural hydroxycinnamic ester with significant applications in food preservation and nutritional health. However, extraction of CGA from plants challenging, resulting low purity that fails to meet increasing market demands. Furthermore, the broad substrate specificity hydroxycinnamoyl-CoA:quinic transferase catalysis generating plethora byproducts, lack NADPH regeneration, presence degrading proteins impede microbial synthesis CGA. This study achieved de novo Yarrowia lipolytica by introducing hydroxylation condensation modules based on screening synthetic pathway genes optimizing parallel promoters. Additionally, an regeneration system was incorporated enhance efficiency hydroxylation, thereby titer 333.16 mg/L. From transcriptome data, 528 significantly upregulated were identified, deletion YALI0_B21824g slowed rate degradation, which increased 351.33 mg/L shake flasks. Applying fed-batch fermentation 5 L bioreactor further production 4837.32 (64 mg/g DCW). established Y. lipolytica, providing foundation for coumaric its derivatives.

Язык: Английский

Процитировано

1

Multidimensional engineering of Escherichia coli for efficient synthesis of L-tryptophan DOI
Mi Tang, Xuewei Pan,

Tianjin Yang

и другие.

Bioresource Technology, Год журнала: 2023, Номер 386, С. 129475 - 129475

Опубликована: Июль 13, 2023

Язык: Английский

Процитировано

20

Caffeic acid production from glucose using metabolically engineered Escherichia coli DOI Creative Commons

Kosuke Sakae,

Daisuke Nonaka,

Mayumi Kishida

и другие.

Enzyme and Microbial Technology, Год журнала: 2023, Номер 164, С. 110193 - 110193

Опубликована: Янв. 5, 2023

Язык: Английский

Процитировано

17