Energies,
Год журнала:
2023,
Номер
16(22), С. 7606 - 7606
Опубликована: Ноя. 16, 2023
Abundant
availability
of
lignocellulosic
biomass
(LCB)
coupled
with
diverse
pretreatment
methods
have
made
it
a
promising
option
for
energy
production.
However,
faces
several
challenges,
some
which
can
be
overcome
by
integrating
processes.
The
present
study
aims
to
optimize
the
integration
two
different
methods—torrefaction
(to
reduce
moisture
content
and
fractionate
biomass)
alkaline
wood
waste
delignify
biomass)—and
utilize
bioethanol
Pretreatment
performance
was
evaluated
based
on
delignification,
hydrolysis,
Initially,
torrefaction
performed
in
continuous
reactor
at
temperature
range
225–300
°C,
followed
optimization
critical
parameters
torrefied
(TWW),
that
is,
temperature,
reaction
time,
solid–liquid
ratio,
alkali
concentration.
Subsequently,
chemical
carbohydrate
compositions
raw
(RWW)
TWW
were
studied,
enzymatic
hydrolysis
fermentation.
Integrated
positively
impacted
cellulose
glucose
contents
lower
temperatures.
treated
produced
higher
levels
than
(stand-alone)
TWW.
These
results
used
as
basis
choosing
most
suitable
enhanced
conversion.
Preparative Biochemistry & Biotechnology,
Год журнала:
2025,
Номер
unknown, С. 1 - 8
Опубликована: Янв. 17, 2025
Myo-inositol
is
an
active
sugar
alcohol
which
has
important
physiological
functions.
In
this
study,
engineered
strain
that
could
simultaneously
utilize
glucose
and
xylose
to
produce
myo-inositol
was
constructed,
its
fermentation
performance
determined.
Firstly,
the
ptsG
gene
deleted
make
E.
coli
BL21
capable
of
utilizing
as
mixed
carbon
source.
Galp
glk
genes
were
introduced
promote
absorption
after
knockout.
Secondly,
ino1
from
Saccharomyces
cerevisiae
SC288
suhB
overexpressed
construct
complete
biosynthetic
pathway
in
BL21.
Ultimately,
when
20
g/L
with
a
ratio
3:2
used
source,
consumption
rate
total
fastest,
yield
0.63
50
mL/250
mL
culture
system.
When
system
expanded
1
L
shake
flask,
0.69
g/L.
This
study
contributes
production
Frontiers in Industrial Microbiology,
Год журнала:
2024,
Номер
1
Опубликована: Янв. 18, 2024
Lignocellulose
biomass
presents
a
promising
and
renewable
alternative
to
fossil
fuels.
Numerous
engineered
microorganisms
have
been
developed
efficiently
utilize
this
convert
it
into
valuable
platform
chemicals.
This
article
provides
an
overview
of
the
extensive
metabolic
engineering
strategies
employed
create
robust
microbial
cell
factories
for
lignocellulose
biorefinery.
The
focus
lies
on
production
various
chemicals
including
succinic
acid,
lactic
3-hydroxypropinic
xylitol,
biohydrocarbons,
itaconic
2-phenylethanol,
1,2,4-butanetriol,
2,3-butanediol
from
hydrolysate,
especially
hemicellulose.
Additionally,
briefly
discusses
techno-economic
analysis,
challenges,
future
prospects
achieving
more
sustainable
these
Bioresource Technology,
Год журнала:
2024,
Номер
unknown, С. 131951 - 131951
Опубликована: Дек. 1, 2024
Bacteria
species
such
as
E.Coli,
Lactobacilli,
and
pediococci
play
an
important
role
starter
strains
in
fermentation
food
or
polysaccharides
into
lactic
acid.
These
bacteria
were
metabolically
engineered
using
multiple
proven
genome
editing
methods
to
enhance
relevant
phenotypes.
The
efficacy
of
these
procedures
varies
depending
on
the
tool
used
researchers'
ability
pick
suitable
recombinants,
which
significantly
increased
engineering
throughput.
Cyanobacteria
produce
oxygenic
photosynthesis
carbon
dioxide
fixing.
fixed
is
then
retained
cells
metabolised
various
low
molecules
lactate,
succinate,
ethanol.
Lactate
a
building
ingredient
bioplastics,
additives,
medicines.
This
review
covers
recent
advances
acid
production
through
metabolic
genetic
cyanobacteria.
Biotechnology and Bioengineering,
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 30, 2023
High
chiral
purity
of
lactic
acid
is
a
crucial
indicator
for
the
synthesis
lactide
as
primary
intermediate
chemical
ring-open
polymerization
high
molecular
weight
polylactic
(PLA).
Lignocellulose
biomass
most
promising
carbohydrate
feedstock
commercial
production
PLA,
but
presence
trace
d-lactic
in
biorefinery
chain
adversely
affects
and
quality
lactide.
This
study
analyzed
fingerprint
found
that
major
source
comes
from
lignocellulose
feedstock.
The
naturally
occurring
bacteria
water-soluble
carbohydrates
provide
necessary
conditions
generation.
Three
strategies
were
proposed
to
eliminate
generation
pathway
acid,
including
reduction
moisture
content,
conversion
furan
aldehydes
pretreatment,
l-lactic
by
inoculating
engineered
bacteria.
natural
content
during
storage
was
observed
due
lactate
oxidase-catalyzed
oxidation
l-
acids.
provided
an
important
support
cellulosic
with
purity.
