In
this
study,
two
isolated
strains
of
nitrogen-fixing
bacteria,
NF1
(Bacillus
subtilis)
and
NF2
(Azotobacter
chroococcum),
were
added
to
cow
dung
compost
in
individual
(NF1,
NF2)
mixing
(NF3;
with
a
ratio
1:1).
The
results
revealed
that
the
total
nitrogen
levels
NF1,
NF3
treatments
increased
by
44.71%,
38.43%
55.35%,
respectively,
when
compared
Control
(without
bacteria
addition).
Meanwhile,
thermophilic
period
3
treated
groups
was
prolonged,
NH3
N2O
emissions
significant
reduced,
especially
for
NF-3
treatment.
Firmicutes
preponderant
stage,
while
Chloroflexi,
Proteobacteria
Bacteroidetes
mature
stage.
These
conformed
addition
composting
improved
bacterial
community
structure
benefited
retention.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 1, 2025
Strain
ALG19,
a
predominant
culturable
bacterium
isolated
from
the
larval
gut
of
emerald
ash
borer
(Agrilus
planipennis)
infesting
velvet
(Fraxinus
velutina),
was
investigated
to
determine
its
taxonomic
identity
and
evaluate
cellulose-degrading
potential.
The
classification
ALG19
determined
through
whole-genome
sequencing,
average
nucleotide
(ANI)
analysis,
phylogenetic
reconstruction
based
on
single-copy
orthologous
genes.
Functional
annotation
carbohydrate-active
genes
performed
using
COG,
KEGG,
CAZy
databases.
Cellulolytic
activity
assessed
multi-faceted
approach.
First,
carboxymethyl
cellulose
hydrolysis
assays
were
conducted
cellulolytic
capability.
Additionally,
filter
paper
degradation
utilization
phloem
examined.
For
these
experiments,
strain
cultured
in
an
inorganic
salt
medium
supplemented
with
respective
substrates
for
60
days.
Genomic
analyses
confirmed
that
belongs
Delftia
tsuruhatensis.
harbors
283
COG-annotated
associated
carbohydrate
transport
metabolism,
355
KEGG
involved
metabolism
pathways,
105
CAZy-annotated
enzymes.
Phenotypic
revealed
zone
ratio
1.74.
After
60-day
incubation
period,
completely
decomposed
strips
into
flocs,
resulting
38.06%
reduction
dry
weight
compared
control
samples,
which
basically
retained
their
original
shape.
Furthermore,
degraded
cellulose,
leaving
residual
content
69.91%.
This
15.60%
lower
than
control,
exhibited
82.83%.
These
findings
demonstrate
D.
tsuruhatensis
is
capable
degrading
present
host
plant
borer,
insect.
study
identifies
potential
target
microorganism
future
pest
management
strategies,
could
mitigate
damage
caused
by
impairing
digestive
capacity.
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 25, 2025
Abstract
The
rich
cellulose
in
rice
straw
is
an
obstacle
to
the
resource
utilization
of
straw.
It
difficult
degrade
only
by
biodegradation,
chemical
or
physical
degradation.
weight
loss
rate
reached
38.86%
inoculation
Bacillus
amyloliquefaciens
N5
combined
with
alkali
pretreatment.
fermentation
broth
strain
and
could
promote
seed’s
germination
growth
seedlings.
Moreover,
addition
peptone
as
a
nutrient
source
further
amplified
whole
genome
analysis
revealed
genes
associated
degradation
N5,
including
glycoside
hydrolase
family
1
(GH1),
GH13,
GH43,
three
polysaccharide
lyases
(PLs),
eleven
carbohydrate
esterases
(CEs),
twenty-six
glycosyltransferases
(GTs),
twenty-five
carbohydrate-binding
modules
(CBMs).
Bioresource Technology,
Год журнала:
2023,
Номер
393, С. 130097 - 130097
Опубликована: Ноя. 25, 2023
A
simplified
inoculum
agent,
only
comprising
Bacillus
subtilis
and
Aspergillus
niger,
was
utilized
for
industrial-scale
cow-manure
composting
to
investigate
its
impact
on
performance
microbiome.
Inoculants
elevated
the
average
peak
temperatures
by
up
7
10
°C,
respectively,
during
thermophilic
stage,
reduced
organic
matter
content,
raised
germination
index.
Inoculation
also
extended
period
of
above
50
°C
from
12
26
days.
Sequencing
unveiled
significant
shifts
in
microbial
diversity,
composition,
function.
thrived
mesophilic
phase,
potentially
initiating
composting,
whereas
Bacillus,
Lysinibacillus,
Clostridium
were
enriched
stage.
Metagenomic
sequencing
revealed
an
increased
abundance
carbohydrate-active
enzymes
glycometabolism-related
genes
responsible
lignocellulose
degradation
heat
generation
after
inoculation.
These
microbes
functional
contributed
temperature
maintenance
expediting
composting.
This
suggests
effectiveness
this
industrial-level
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(24), С. 13541 - 13541
Опубликована: Дек. 18, 2024
Microbial
attack,
particularly
fungal
degradation
of
cellulose,
is
a
leading
cause
paper
damage.
To
address
spores
and
the
rising
concern
microbial
drug
resistance,
nano-Germanium
dioxide
(GeO2)/cetyltrimethylammonium
bromide
(CTAB)
complex
(nano-GeO2/CTAB
complex)
with
potent
antibacterial
properties
was
synthesized.
Its
inhibitory
effects
were
evaluated
against
bacteria,
including
Gram-positive
Staphylococcus
aureus
Gram-negative
Escherichia
coli,
as
well
fungi
isolated
from
(Fusarium
spp.,
Aspergillus
Penicillium
citrinum).
The
nano-GeO2/CTAB
exhibited
significant
(p
<
0.05)
S.
E.
coli.
Moreover,
60
min
treatment
1
mg/mL
significantly
inhibited
growth
all
tested
reduced
their
biomass
after
five
days
culture,
while
4
completely
deactivated
spores.
Filter
pre-treated
showed
complete
resistance
to
exhibiting
no
clear
inhibition
zone
devoid
bacterial
growth.
In
contrast,
untreated
controls
displayed
coverage
exceeding
95%
within
days.
These
findings
highlight
promising
antimicrobial
agent
for
protecting
materials
degradation.
Regarding
effective
lignocellulose
degradation,
six
functional
strains
were
isolated
from
several
organic
sources.
Among
them,
Trichoderma
atroviride
(LH-413)
stood
out
due
to
its
highly
developed
nations
enzymatic
system,
which
could
detect
cellulase
and
ligninase,
with
an
astounding
filter
paper
weight
loss
of
about
68%.
Isolated
randomly
constructed
laboratory
preserved
Phanerochaete
chrysosporium
longibrachiatum
obtain
optimal
set
composite
LXB,
overall
improvement
in
enzyme
activity
a
lignocellulosic
degradation
rate
40%.
Metabolic
function
predictions
indicate
that
carbohydrate
metabolism
amino-acid
have
considerable
gene
abundance
are
the
major
metabolic
pathways.
Plant
Pathogen–Undefined
Saprotroph
Undefined
predominate,
contributing
saprotrophic
role
LXB.
Therefore,
LXB
may
be
employed
future
as
excellent
bioproduct
address
problem
biodegradation,
it
used
applications
involving
composting.
The
biodegradation
of
lignocellulosic
biomass
is
a
practical
and
effective
method.
A
random
combination
six
isolates
two
purchased
strains
was
constructed
to
obtain
LXB,
the
degradation
lignocellulose
resulted
in
40.6%
after
168
hours.
total
1872
genes
that
were
differentially
expressed
involved
LXB
have
been
identified
Carbohydrate-Active
enZYmes
Database.
Genes
encode
cellulases
hemicellulase
abundance,
gene
encodes
displaying
highest
expression
at
initial
peak
stages
degradation.
Considerable
increase
Versatile
peroxidase
coenzyme
Alcohol
oxidase
lignin
catabolism.
catalyzes
breakdown
mainly
through
pathways
carbohydrate
metabolic
pathway,
catechol
discovered
benzoate
which
participates
lignin.
Additionally,
possesses
capability
degrade
harmful
exogenous
substances.
