bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 18, 2023
Abstract
Background
The
integration
of
anaerobic
digestion
into
bio-based
industries
can
create
synergies,
which
help
to
make
self-sustaining.
Two-stage
digesters
with
separate
acidification
stages
allow
produce
green
hydrogen
and
short-chain
fatty
acids,
are
promising
industrial
products.
Heat
shocks
be
used
foster
the
production
these
However,
practical
applicability
is
oft
not
addressed
sufficiently.
here
presented
work
aims
close
this
gap.
Methods
Batch
experiments
were
conducted
in
5
litre
double-walled
tank
reactors
incubated
at
37
°C.
Short
microwave
heat
25
min
duration
exposure
times
-
10
80
°C
performed
compared
oven
shocks.
Pairwise
experimental
group
differences
for
gas
chemical
parameters
determined
using
ANOVA
post-hoc
tests.
High-throughput
16S
rRNA
gene
amplicon
sequencing
was
analyse
taxonomic
profiles.
Results
shocking
entire
seed
sludge,
highest
productivity
observed
a
substrate
load
50
g/l
1.09
mol
H
2
/mol
hexose.
With
1.01
hexose,
assisted
treatment
significantly
different
from
oven-based
treatments.
study
emphasised
better
repeatability
microwave-assisted
experiments,
showing
low
variation
coefficients
averaging
29
%.
Microwave
pre-treatment
indicates
high
predictability
stronger
microbiome
shift
Clostridia
than
oven.
supported
formation
butyric
acid
up
10.8
average
peak
24.01
butyric/acetic
ratio
2.0.
Conclusion
show
suitability
shock
sludge
rather
just
small
inoculum,
makes
process
more
relevant
application.
A
microwave-based
has
proven
alternative
treatments,
ultimately
might
facilitate
implementation
systems.
approach
becomes
economically
sustainable
high-temperature
pumps
coefficient
performance
(COP)
4.3.
Polymers,
Год журнала:
2024,
Номер
16(7), С. 893 - 893
Опубликована: Март 25, 2024
Water
scarcity
is
a
significant
global
issue
caused
by
the
prolonged
disregard
and
unsustainable
management
of
this
essential
resource
both
public
private
bodies.
The
dependence
on
fossil
fuels
further
exacerbates
society’s
bleak
environmental
conditions.
Therefore,
it
crucial
to
explore
alternative
solutions
preserve
our
nation’s
water
resources
properly
promote
production
biofuels.
Research
into
utilization
E.
crassipes
remove
heavy
metals
generate
biofuels
extensive.
combination
these
two
lines
inquiry
presents
an
excellent
opportunity
achieve
sustainable
development
goals.
This
study
aims
develop
wastewater
treatment
system
biohydrogen
from
dry,
pulverized
biomass.
A
was
implemented
treat
1
L
industrial
waste.
interconnected
compartment
built
utilizing
recycled
PET
bottles
reusing
feedstock
for
process.
biological
hydrogen
through
dark
fermentation,
using
biomass
containing
as
source,
studied.
Cr
(VI)
Pb
(II)
levels
had
low
impact
production.
uncontaminated
displayed
significantly
higher
yield
(81.7
mL
H2/g
glucose).
presence
(IV)
in
leads
decrease
14%,
26%.
work
proposes
strategy
that
utilizes
green
technologies
recover
utilize
contaminated
water.
Additionally,
enables
bioenergy
with
high
efficiency,
indirectly
reducing
greenhouse
gases.
aligns
international
programs
circular
economy.
Frontiers in Industrial Microbiology,
Год журнала:
2024,
Номер
2
Опубликована: Апрель 25, 2024
Climate
change
and
pollution
drive
the
need
for
fossil
fuel
alternatives.
Dark
fermentation
offers
promise
through
use
of
microbial
consortia
to
convert
organic
matter
into
hydrogen
gas.
Persisting
challenges
like
instability
low
yields
may
stem
from
reduced
diversity
anaerobic
digestion
communities
that
serve
as
inoculum
undergo
aggressive
pretreatments
culturing
conditions.
This
study
explores
impact
loss
on
function,
focusing
biogas
production
stability.
Two
treatments,
with
without
pretreatment,
were
tested
12
replicate
bioreactors
each,
resulting
in
differing
levels.
Microbial
assessed
via
16S
amplicon
sequencing,
monitoring
production,
volatile
fatty
acids,
testing
invasion
susceptibility.
The
two
treatments
exhibited
divergent
assembly
functional
trajectories,
although
replicates
within
each
treatment
ultimately
converged
similar
compositions
stable
levels
production.
Heat-treated
showed
a
91.5%
increase
but
higher
susceptibility
compared
non-treated.
Non-treated
unique
species
associations
(e.g.
Ethanoligenens
harbinense
Enterococcus
olivae
),
distinct
commonly
studied
Clostridium
group.
These
findings
provide
insights
effects
stability,
elucidating
differences
across
taxonomic
stability
well
Moreover,
identification
novel
bacterial
groups
associated
suggests
promising
directions
future
research
enhance
control
design
dark
fermentation.
Abstract
Hydrogen,
the
simplest
and
most
abundant
element
in
universe,
is
poised
today
to
play
a
critical
role
transition
toward
clean
energy
future.
This
perspective
article
explores
potential
of
hydrogen
as
sustainable
carrier.
First,
historical
modern
applications
across
various
sectors
are
examined.
Then,
delves
into
different
methods
for
production.
Since
conventional
fossil
fuel‐based
production
raise
environmental
concerns,
promising
alternatives
cleaner
generation,
including
electrolysis
powered
by
renewable
energy,
biomass
gasification,
photocatalytic
reforming.
The
technical
details
advantages/disadvantages
discussed,
well
ongoing
research
development
efforts
improve
existing
techniques
make
them
more
sustainable,
efficient,
cost‐effective.
As
world
strives
future,
holds
immense
transformative
role.
Energies,
Год журнала:
2025,
Номер
18(5), С. 1092 - 1092
Опубликована: Фев. 24, 2025
This
study
explores
biohydrogen
production
through
dark
fermentation,
an
alternative
supporting
sustainable
hydrogen
generation.
Dark
fermentation
uses
organic
waste
under
anaerobic
conditions
to
produce
in
the
absence
of
light.
Key
process
parameters
affecting
yield,
including
substrate
type,
microorganism
selection,
and
conditions,
were
examined.
Various
substrates,
such
as
wastes
carbohydrates,
tested,
role
facultative
microorganisms
optimizing
was
analyzed.
The
research
also
focused
on
factors
pH,
temperature,
hydraulic
retention
time
enhance
yields
scalability.
Additionally,
modelled
using
ASPEN
Plus
software
14.
simulation
identifies
bottle
necks
this
process.
Due
lack
available
data,
modelling
described
processes
required
certain
approximations.
provides
insight
into
key
challenges
that
need
be
addressed
for
production.
Future
should
indeed
explore
current
limitations,
efficiency,
scalability,
cost-effectiveness,
well
potential
advancements
like
genetic
engineering
microbial
strains
improved
bioreactor
designs.
Sustainability,
Год журнала:
2024,
Номер
16(23), С. 10755 - 10755
Опубликована: Дек. 8, 2024
Hydrogen
is
becoming
recognized
as
a
clean
and
sustainable
energy
carrier,
with
microbial
fermentation
electrolysis
serving
critical
roles
in
its
production.
This
paper
provides
thorough
meta-analysis
of
BioH2
production
across
diverse
substrates,
populations,
experimental
settings.
Statistical
techniques,
including
ANOVA,
principal
component
analysis
(PCA),
heatmaps,
were
used
to
evaluate
the
influence
various
parameters
on
hydrogen
yield.
The
mean
generation
from
reviewed
studies
was
168.57
±
52.09
mL
H2/g
substrate,
food
waste
glucose
demonstrating
considerably
greater
than
mixed
(p
<
0.05).
inhibition
methanogens
inhibitors
like
2-bromoethanesulfonate
(BES)
chloramphenicol
(CES)
enhanced
by
much
25%,
demonstrated
cell
systems.
PCA
results
highlighted
Clostridium
spp.,
Thermotoga
Desulfovibrio
spp.
most
dominant
species,
contributing
up
80%
YH2
study
highlights
synergistic
interactions
between
less
species
under
optimized
environmental
conditions
(pH
5.5–6.0,
65
°C),
emphasizing
their
complementary
enhancing
H2
Volatile
fatty
acid
regulation,
particularly
acetate
butyrate
accumulation,
correlated
positively
(r
=
0.75,
p
0.01).
These
findings
provide
insights
into
optimizing
biohydrogen
systems
through
consortia
management
substrate
selection,
offering
potential
way
for
scalable
efficient
