Gene editing and its applications in biomedicine

Science China Life Sciences, Год журнала: 2022, Номер 65(4), С. 660 - 700

Опубликована: Фев. 18, 2022

Язык: Английский

---

Boron and Nitrogen-Codoped Carbon Dots as Highly Efficient Electrochemiluminescence Emitters for Ultrasensitive Detection of Hepatitis B Virus DNA

Analytical Chemistry, Год журнала: 2022, Номер 94(21), С. 7601 - 7608

Опубликована: Май 16, 2022

In this work, boron and nitrogen-codoped carbon dots (BN-CDs) as highly efficient electrochemiluminescence (ECL) emitters with advantages of low excitation potential high ECL efficiency were prepared to establish a novel ternary system for ultrasensitive detection HBV-DNA. Especially, both platinum nanoflowers (Pt NFs) radicals (B•) from the BN-CDs could accelerate reduction coreactant S2O82– abundant SO4•– simultaneously, making have outstanding performance. Impressively, is much higher than that nondoped CDs single-doped CDs. addition, by combining exonuclease III (Exo III)-induced target DNA amplification strategy, an biosensor was constructed realize HBV-DNA 100 aM 1 nM, while limit 18.08 aM. Therefore, promising emitter offered develop method clinical disease analysis.

Язык: Английский

---

CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria

Small Methods, Год журнала: 2022, Номер 6(10)

Опубликована: Сен. 16, 2022

Abstract Infectious pathogens cause severe human illnesses and great deaths per year worldwide. Rapid, sensitive, accurate detection of is importance for preventing infectious diseases caused by optimizing medical healthcare systems. Inspired a microbial defense system (i.e., CRISPR/ CRISPR‐associated proteins (Cas) system, an adaptive immune protecting microorganisms from being attacked invading species), many new biosensors have been successfully developed widely applied in the viruses pathogenic bacteria. Moreover, advanced nanotechnologies also integrated into these to improve their stability, sensitivity, accuracy. In this review, recent advance CRISPR/Cas systems‐based nano/biosensors applications bacteria are comprehensively reviewed. First all, categories working principles systems establishing simply introduced. Then, design construction discussed. end, attentions focused on Impressively, remaining opportunities challenges further development system‐based promising proposed.

Язык: Английский

---

Electrochemical detection of ctDNA mutation in non-small cell lung cancer based on CRISPR/Cas12a system

Sensors and Actuators B Chemical, Год журнала: 2022, Номер 362, С. 131807 - 131807

Опубликована: Март 31, 2022

Язык: Английский

---

CRISPR/Cas-Powered Amplification-Free Detection of Nucleic Acids: Current State of the Art, Challenges, and Futuristic Perspectives

ACS Sensors, Год журнала: 2023, Номер 8(12), С. 4420 - 4441

Опубликована: Ноя. 18, 2023

CRISPR/Cas system is becoming an increasingly influential technology that has been repositioned in nucleic acid detection. A preamplification step usually required to improve the sensitivity of CRISPR/Cas-based The striking biological features CRISPR/Cas, including programmability, high and sequence specificity, single-base resolution. More strikingly, target-activated trans-cleavage could act as a biocatalytic signal transductor amplifier, thereby empowering it potentially perform detection without step. reports such work are on rise, which not only scientifically significant but also promising for futuristic end-user applications. This review started with introduction methods acids diagnostics (CRISPR-Dx). Next, we objectively discussed pros cons steps CRISPR-Dx. We then illustrated highlighted recently developed strategies CRISPR/Cas-powered amplification-free can be realized through uses ultralocalized reactors, cascade reactions, ultrasensitive systems, or others. Lastly, challenges perspectives were proposed. It expected this makes researchers better understand current emerging field, provides insight designing novel CRISPR-Dx win practicable use near future.

Язык: Английский

---

Gold Nanomaterials‐Implemented CRISPR‐Cas Systems for Biosensing

Small, Год журнала: 2023, Номер 19(21)

Опубликована: Фев. 25, 2023

Due to their superiority in the simple design and precise targeting, clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have attracted significant interest for biosensing. On one hand, CRISPR-Cas capacity precisely recognize cleave specific DNA RNA sequences. other such as orthologs of Cas9, Cas12, Cas13 exhibit cis-cleavage or trans-cleavage activities after recognizing target sequence. Owing cleavage activities, can be designed biosensing by degrading tagged nucleic acids produce detectable signals. To meet requirements point-of-care detection versatile signal readouts, gold nanomaterials with excellent properties high extinction coefficients, easy surface functionalization, biocompatibility are implemented CRISPR-Cas-based biosensors. In combination nanoparticles, nanorods, nanostars, great efforts devoted fabricating biosensors diverse targets. This review focuses on current advances nanomaterials-implemented biosensors, particularly working mechanism performance these systems, including CRISPR-Cas9, CRISPR-Cas12a, CRISPR-Cas13a discussed highlighted. Meanwhile, prospects challenges also strategies based systems.

Язык: Английский

---

Mesoporous Nanozyme-Enhanced DNA Tetrahedron Electrochemiluminescent Biosensor with Three-Dimensional Walking Nanomotor-Mediated CRISPR/Cas12a for Ultrasensitive Detection of Exosomal microRNA

Analytical Chemistry, Год журнала: 2023, Номер 95(9), С. 4486 - 4495

Опубликована: Фев. 20, 2023

Exosomal microRNAs (exomiRNAs) have emerged as ideal biomarkers for early clinical diagnostics. The accurate detection of exomiRNAs plays a crucial role in facilitating applications. Herein, an ultrasensitive electrochemiluminescent (ECL) biosensor was constructed using three-dimensional (3D) walking nanomotor-mediated CRISPR/Cas12a and tetrahedral DNA nanostructures (TDNs)-modified nanoemitters (TCPP-Fe@HMUiO@Au-ABEI) exomiR-155 detection. Initially, the 3D strategy could effectively convert target into amplified biological signals improving sensitivity specificity. Then, TCPP-Fe@HMUiO@Au nanozymes with excellent catalytic performance were used to amplify ECL because enhanced mass transfer increased active sites, originating from its high surface areas (601.83 m2/g), average pore size (3.46 nm), large volumes (0.52 cm3/g). Meanwhile, TDNs scaffold fabricate "bottom-up" anchor bioprobes improve trans-cleavage efficiency Cas12a. Consequently, this achieved limit down 273.20 aM ranging 1.0 fM nM. Furthermore, discriminate breast cancer patients evidently by analyzing exomiR-155, these results conformed that qRT-PCR. Thus, work provides promising tool

Язык: Английский

---

Competition between enzymatic and non-enzymatic electrochemical determination of cholesterol

Journal of Electroanalytical Chemistry, Год журнала: 2023, Номер 930, С. 117169 - 117169

Опубликована: Янв. 13, 2023

Язык: Английский

---

CRISPR-Cas12a Biosensor Array for Ultrasensitive Detection of Unamplified DNA with Single-Nucleotide Polymorphic Discrimination

ACS Sensors, Год журнала: 2023, Номер 8(4), С. 1489 - 1499

Опубликована: Апрель 7, 2023

Quantitative polymerase chain reaction as a powerful tool for DNA detection has been pivotal to vast range of applications, including disease screening, food safety assessment, environmental monitoring, and many others. However, the essential target amplification step in combination with fluorescence readout poses significant challenge rapid streamlined analysis. The discovery engineering clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated (Cas) technology have recently paved way novel approach nucleic acid detection, but majority current CRISPR-mediated platforms are limited by insufficient sensitivity still require preamplification. Herein, we report CRISPR-Cas12a-mediated graphene field-effect transistor (gFET) array, named CRISPR Cas12a-gFET, amplification-free, ultrasensitive, reliable both single-stranded (ssDNA) double-stranded (dsDNA) targets. Cas12a-gFET leverages multiturnover trans-cleavage activity Cas12a intrinsic signal ultrasensitivity gFET. As demonstrated, achieves limit 1 aM ssDNA human papillomavirus 16 synthetic 10 dsDNA Escherichia coli plasmid without In addition, an array 48 sensors on single 1.5 cm × chip is employed improve data reliability. Finally, demonstrates capability discriminate single-nucleotide polymorphisms. Together, biosensor provides reliable, highly specific detections.

Язык: Английский

---

Regulation of Target-activated CRISPR/Cas12a on Surface Binding of Polymer Dots for Sensitive Electrochemiluminescence DNA Analysis

Analytical Chemistry, Год журнала: 2023, Номер 95(18), С. 7396 - 7402

Опубликована: Апрель 29, 2023

Polymer dots (Pdots) have emerged as a type of attractive electrochemiluminescence (ECL) emitter. However, the low ECL efficiency severely limits their practicability. In this work, we develop sensitive biosensing strategy for detection human papilloma virus subtype (HPV-16) DNA by using target-activated CRISPR/Cas12a to regulate binding Pdots-DNA biosensor and local surface plasmon resonance (LSPR) effect electrochemically deposited Au nanoparticles (depAuNPs) enhance emission Pdots bound on biosensor. The is prepared simply assembling hairpin depAuNPs modified electrode. presence target DNA, designed specific can be activated digest single-stranded assistant which decreases amount opened bind surface, thus reduces emission. integration DNA-triggered catalysis LSPR greatly improves sensitivity analysis. Using HPV-16 model, proposed method shows limit (LOD) 3.2 fM at signal-to-noise ratio 3 detectable concentration range 5.0 50 pM. high sensitivity, excellent selectivity, good testing stability, acceptable fabrication reproducibility demonstrate its potential application in bioanalysis.

Язык: Английский

---