Recent progress on microfluidic biosensors for rapid detection of pathogenic bacteria

Chinese Chemical Letters, Год журнала: 2021, Номер 33(4), С. 1743 - 1751

Опубликована: Авг. 19, 2021

Язык: Английский

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A Universal Bacterial Catcher Au–PMBA-Nanocrab-Based Lateral Flow Immunoassay for Rapid Pathogens Detection

Analytical Chemistry, Год журнала: 2022, Номер 94(10), С. 4277 - 4285

Опубликована: Март 4, 2022

In traditional lateral flow immunoassays (LFIA) for pathogens detection, capture antibody (CA) is necessary and usually conjugated to Au nanoparticles (NPs) in order label the target analyte. However, acquisition process of Au–CA nanoprobe relatively complicated costly, which will limit application LFIA. Herein, p-mercaptophenylboronic acid-modified NPs (namely Au–PMBA nanocrabs), were synthesized applied a new CA-independent LFIA method. The stable nanocrabs showed outstanding capability both Gram-negative bacteria Gram-positive through covalent bonding. acquired Au–PMBA–bacteria complexes dropped onto strip, then captured by detection on test line (T-line). Take Escherichia coli O157:H7 as an example, gray value T-line was proportional concentration linear range 103–107 cfu·mL–1. This strategy exhibited higher sensitivity than CA-dependent double sandwich method, because former one 103 cfu·mL–1 only visual observation, reduced 3 orders magnitude. Besides, this platform successfully screened E. four food samples with recoveries ranging from 90.25% 107.25%. great advantages satisfactory potential rapid foodborne real samples.

Язык: Английский

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Recent advances of fluorescent sensors for bacteria detection-A review

Talanta, Год журнала: 2022, Номер 254, С. 124133 - 124133

Опубликована: Ноя. 25, 2022

Язык: Английский

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Material Breakthroughs in Smart Food Monitoring: Intelligent Packaging and On‐Site Testing Technologies for Spoilage and Contamination Detection

Advanced Materials, Год журнала: 2023, Номер 36(1)

Опубликована: Апрель 22, 2023

Abstract Despite extensive commercial and regulatory interventions, food spoilage contamination continue to impose massive ramifications on human health the global economy. Recognizing that such issues will be significantly eliminated by accurate timely monitoring of quality markers, smart sensors have garnered significant interest as platforms for both real‐time, in‐package on‐site testing. In cases, sensitivity, stability, efficiency developed are largely informed underlying material design, driving focus toward creation advanced materials optimized applications. Herein, a comprehensive review emerging intelligent in this space is provided, through lens three key markers – biogenic amines, pH, pathogenic microbes. Each sensing platform presented with targeted consideration contributions metallic or polymeric substrate mechanism detection performance. Further, real‐world applicability works considered respect their capabilities, adherence, potential. Finally, situational assessment current state technologies discussing material‐centric strategies address existing limitations, concerns, considerations.

Язык: Английский

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Recombinant antibodies and their use for food immunoanalysis

Analytical and Bioanalytical Chemistry, Год журнала: 2021, Номер 414(1), С. 193 - 217

Опубликована: Авг. 21, 2021

Язык: Английский

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Recent advances in the analytical strategies of microbial biosensor for detection of pollutants

Chemosphere, Год журнала: 2022, Номер 306, С. 135515 - 135515

Опубликована: Июнь 27, 2022

Язык: Английский

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Bacteriophage-Based Biosensors: A Platform for Detection of Foodborne Bacterial Pathogens from Food and Environment

Biosensors, Год журнала: 2022, Номер 12(10), С. 905 - 905

Опубликована: Окт. 21, 2022

Foodborne microorganisms are an important cause of human illness worldwide. Two-thirds foodborne diseases caused by bacterial pathogens throughout the globe, especially in developing nations. Despite enormous developments conventional pathogen detection methods, progress is limited assay complexity and a prolonged time-to-result. The specificity sensitivity assays for live may also depend on nature samples being analyzed immunological or molecular reagents used. Bacteriophage-based biosensors offer several benefits, including to their host organism, only pathogens, resistance extreme environmental factors such as organic solvents, high temperatures, wide pH range. Phage-based receiving increasing attention owing degree accuracy, specificity, reduced times. These characteristics, coupled with abundant supply, make phages novel bio-recognition molecule development, ensure food safety. This review provides comprehensive information about different types phage-based biosensor platforms, magnetoelastic sensors, quartz crystal microbalance, electrochemical surface plasmon resonance from various representative matrices samples.

Язык: Английский

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Phage Adsorption to Gram-Positive Bacteria

Viruses, Год журнала: 2023, Номер 15(1), С. 196 - 196

Опубликована: Янв. 10, 2023

The phage life cycle is a multi-stage process initiated by the recognition and attachment of virus to its bacterial host. This adsorption step depends on specific interaction between structures acting as receptors viral proteins called Receptor Binding Proteins (RBP). essential it first determinant host range sine qua non condition for subsequent conduct cycle. In phages belonging

Язык: Английский

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Lateral Flow Strip Biosensors for Foodborne Pathogenic Bacteria via Direct and Indirect Sensing Strategies: A Review

Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(27), С. 10250 - 10268

Опубликована: Июнь 30, 2023

Rapid and sensitive detection of foodborne pathogenic bacteria is particularly important for the prevention control diseases. The lateral flow strip biosensor (LFSB) one most promising point-of-care tools has been widely used in food safety monitoring. This review introduces recent advances using LFSBs. According to different bacterial biomarkers, we summarize direct indirect sensing strategies whole cells are divided into antibodies, antibody alternatives, label-free according recognition elements. refer nucleic acids metabolites. Next, compare discuss applications strategies. Finally, existing challenges, future perspectives, development directions discussed, which will facilitate theoretical innovation practical application

Язык: Английский

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Genetic engineering of bacteriophages: Key concepts, strategies, and applications

Biotechnology Advances, Год журнала: 2023, Номер 64, С. 108116 - 108116

Опубликована: Фев. 10, 2023

Язык: Английский

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