Abstract
Peroxidase
(POD)
Nanozyme‐based
hydrogen
peroxide
(H
2
O
)
detection
is
popular,
but
hardly
adapt
to
high
concentration
of
H
owing
narrow
linear
range
(LR)
and
low
LR
maximum.
Here,
a
solution
combining
POD
catalase
(CAT)
raised
expand
the
assay
via
decomposing
part
.
As
proof
concept,
cascade
enzyme
system
(rGRC)
constructed
by
integrating
ruthenium
nanoparticles
(RuNPs),
CAT
graphene
together.
The
rGRC‐based
sensor
does
perform
an
expanded
higher
maximum
for
detection.
Meanwhile,
it
confirmed
that
expansion
closely
associated
with
apparent
K
m
rGRC,
which
determined
relative
activity
between
both
in
theory
experiment.
At
last,
rGRC
successfully
used
detect
(up
10
contact
lens
care
solution,
performs
accuracy
(close
100%
recovery
at
than
traditional
nanozymes.
This
study
brings
up
kind
POD/CAT
provides
new
concept
accurate
facile
Additionally,
replenishes
enzyme‐substrate
model
achieving
same
pattern
competitive
inhibition
reactions.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(38), С. 14494 - 14501
Опубликована: Сен. 14, 2023
Noble-metal
nanozymes
have
demonstrated
great
potential
in
various
fields.
However,
aggregation
of
single-particle
nanoparticles
severely
affects
their
exposed
catalytically
active
sites
to
the
extent
exhibiting
weak
enzyme-like
activity.
Here,
we
present
an
organic
block
surfactant
(polyvinylpyrrolidone,
PVP)
construct
monodisperse
water-stable
Pt
nanoclusters
(Pt
NCs)
for
enhanced
immunoassay
cardiac
troponin
I
(cTnI).
The
PVP-modified
NC
nanozyme
exhibited
up
16.3
U
mg-1
peroxidase-mimicking
activity,
which
was
mainly
attributed
ligand
modification
on
surface
and
electron-absorbing
effect
NCs.
NCs
a
lower
OH-transition
potential,
as
determined
by
density
functional
theory.
Under
optimized
experimental
conditions,
strategy
ultrawide
dynamic
response
range
0.005-50
ng
mL-1
cTnI
targets
with
detection
limit
1.3
pg
mL-1,
far
superior
some
reported
test
protocols.
This
work
provides
designable
pathway
design
artificial
enzymes
high
activity
further
expand
practical
enzyme
alternatives.
Sensors,
Год журнала:
2023,
Номер
23(5), С. 2749 - 2749
Опубликована: Март 2, 2023
Colorimetric
sensors
have
been
widely
used
to
detect
numerous
analytes
due
their
cost-effectiveness,
high
sensitivity
and
specificity,
clear
visibility,
even
with
the
naked
eye.
In
recent
years,
emergence
of
advanced
nanomaterials
has
greatly
improved
development
colorimetric
sensors.
This
review
focuses
on
(from
years
2015
2022)
advances
in
design,
fabrication,
applications
First,
classification
sensing
mechanisms
are
briefly
described,
design
based
several
typical
nanomaterials,
including
graphene
its
derivatives,
metal
oxide
nanoparticles,
DNA
quantum
dots,
some
other
materials
discussed.
Then
applications,
especially
for
detection
metallic
non-metallic
ions,
proteins,
small
molecules,
gas,
virus
bacteria,
DNA/RNA
summarized.
Finally,
remaining
challenges
future
trends
also
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Ноя. 6, 2023
Inspired
by
structures
of
natural
metalloenzymes,
a
biomimetic
synthetic
strategy
is
developed
for
scalable
synthesis
porous
Fe-N3
single
atom
nanozymes
(pFeSAN)
using
hemoglobin
as
Fe-source
and
template.
pFeSAN
delivers
3.3-
8791-fold
higher
oxidase-like
activity
than
Fe-N4
Fe3O4
nanozymes.
The
high
catalytic
performance
attributed
to
(1)
the
suppressed
aggregation
atomically
dispersed
Fe;
(2)
facilitated
mass
transfer
maximized
exposure
active
sites
created
mesopores
thermal
removal
(2
~
3
nm);
(3)
unique
electronic
configuration
oxygen-to-water
oxidation
pathway
(analogy
with
cytochrome
c
oxidase).
successfully
demonstrated
rapid
colorimetric
detection
glutathione
low
limit
(2.4
nM)
wide
range
(50
nM-1
mM),
further
real-time,
facile,
(~6
min)
precise
visualization
analysis
methodology
tumors
via
level,
showing
its
potentials
diagnostic
clinic
applications.
Advanced Materials,
Год журнала:
2023,
Номер
36(10)
Опубликована: Апрель 27, 2023
Nanozymes
represent
a
class
of
nanosized
materials
that
exhibit
innate
catalytic
properties
similar
to
biological
enzymes.
The
unique
features
these
have
positioned
them
as
promising
candidates
for
applications
in
clinical
sensing
devices,
specifically
those
employed
at
the
point-of-care.
They
notably
found
use
means
amplify
signals
nanosensor-based
platforms
and
thereby
improve
sensor
detection
limits.
Recent
developments
understanding
fundamental
chemistries
underpinning
enabled
development
highly
effective
nanozymes
capable
clinically
relevant
biomarkers
limits
compete
with
"gold-standard"
techniques.
However,
there
remain
considerable
hurdles
need
be
overcome
before
nanozyme-based
sensors
can
utilized
platform
ready
use.
An
overview
current
understandings
disease
diagnostics
biosensing
unmet
challenges
must
considered
prior
their
translation
diagnostic
tests
is
provided.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(33), С. 13663 - 13671
Опубликована: Авг. 10, 2024
Rapid
and
accurate
detection
of
human
epidermal
growth
factor
receptor
2
(HER2)
is
crucial
for
the
early
diagnosis
prognosis
breast
cancer.
In
this
study,
we
reported
an
iron-manganese
ion
N-doped
carbon
single-atom
catalyst
(FeMn-NC
Analytical Chemistry,
Год журнала:
2023,
Номер
95(23), С. 8906 - 8913
Опубликована: Июнь 2, 2023
Developing
highly
active
and
sensitive
nanozymes
for
biothiol
analysis
is
of
vital
significance
due
to
their
essential
roles
in
disease
diagnosis.
Herein,
two
metal
ion-doped
carbon
dots
(M-CDs)
with
high
peroxidase-like
activity
were
designed
prepared
detection
identification
through
the
colorimetric
sensor
array
technique.
The
M-CDs
can
strongly
catalyze
decomposition
H2O2,
accompanied
by
color
changes
3,3′,5,5′-tetramethylbenzidine
(TMB)
from
colorless
blue,
indicating
peroxidase-mimicking
activities
M-CDs.
Compared
pure
(CDs),
exhibited
enhanced
owing
synergistic
effect
between
ions
CDs.
However,
strong
binding
affinity
biothiols
ions,
catalytic
could
be
inhibited
different
diverse
degrees.
Therefore,
using
TMB
as
a
chromogenic
substrate
presence
developed
form
differential
fingerprints
three
most
important
(i.e.,
cysteine
(Cys),
homocysteine
(Hcy),
glutathione
(GSH)),
which
accurately
discriminated
pattern
recognition
methods
hierarchical
clustering
(HCA)
principal
component
(PCA))
limit
5
nM.
Moreover,
single
various
concentrations
mixtures
was
also
realized.
Furthermore,
actual
samples
such
cells
sera
well
distinguished
as-fabricated
array,
demonstrating
its
potential
ACS Nano,
Год журнала:
2024,
Номер
18(19), С. 12049 - 12095
Опубликована: Май 2, 2024
Cancer,
as
one
of
the
leading
causes
death
worldwide,
drives
advancement
cutting-edge
technologies
for
cancer
treatment.
Transition-metal-based
nanozymes
emerge
promising
therapeutic
nanodrugs
that
provide
a
reference
therapy.
In
this
review,
we
present
recent
breakthrough
First,
comprehensively
outline
preparation
strategies
involved
in
creating
transition-metal-based
nanozymes,
including
hydrothermal
method,
solvothermal
chemical
reduction
biomimetic
mineralization
and
sol–gel
method.
Subsequently,
elucidate
catalytic
mechanisms
(catalase
(CAT)-like
activities),
peroxidase
(POD)-like
oxidase
(OXD)-like
activities)
superoxide
dismutase
(SOD)-like
along
with
their
activity
regulation
such
morphology
control,
size
manipulation,
modulation,
composition
adjustment
surface
modification
under
environmental
stimulation.
Furthermore,
elaborate
on
diverse
applications
anticancer
therapies
encompassing
radiotherapy
(RT),
chemodynamic
therapy
(CDT),
photodynamic
(PDT),
photothermal
(PTT),
sonodynamic
(SDT),
immunotherapy,
synergistic
Finally,
challenges
faced
by
are
discussed
alongside
future
research
directions.
The
purpose
review
is
to
offer
scientific
guidance
will
enhance
clinical
based
transition
metals.
ACS Applied Materials & Interfaces,
Год журнала:
2023,
Номер
15(5), С. 6473 - 6485
Опубликована: Янв. 31, 2023
The
residues
of
organophosphorus
pesticides
(OPs)
have
drawn
worldwide
increasing
attention
because
their
potential
fatal
effects
on
human
health
and
ecological
systems.
It
is
great
significance
to
develop
an
efficient
portable
method
for
in-field
detection
OPs.
Herein,
a
novel
core-shell
nanocomposite
prussian
blue@Fe-covalent
organic
framework@Au
(PB@Fe-COF@Au)
was
constructed.
Fe2+
Fe3+
in
PB
nanoparticle
(PBNP)
cores,
Fe-porphyrin
COF
shells,
AuNPs
grown
shells
all
acted
as
peroxidase-like
catalytic
active
sites,
enabling
PB@Fe-COF@Au
possess
triplet
activity.
A
colorimetric,
affordable,
sensitive,
selective
strategy
designed
detect
Compared
with
previous
reports,
this
sensor
realized
wider
linear
range
chlorpyrifos
10-800
ng
mL-1
relatively
lower
limit
0.61
mL-1,
which
attributed
the
overlapping
triple
sites
response
assay
successfully
employed
food
environmental
samples.
Moreover,
meet
demand
OPs,
spherical
hydrogel
based
visual,
portable,
equipment-free
features
fabricated.
This
work
provides
new
pathway
design
apply
effective
nanozymes
on-site
monitoring
pesticides.
