Block-Polymer-Restricted Sub-nanometer Pt Nanoclusters Nanozyme-Enhanced Immunoassay for Monitoring of Cardiac Troponin I

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(38), P. 14494 - 14501

Published: Sept. 14, 2023

Noble-metal nanozymes have demonstrated great potential in various fields. However, aggregation of single-particle nanoparticles severely affects their exposed catalytically active sites to the extent exhibiting weak enzyme-like activity. Here, we present an organic block surfactant (polyvinylpyrrolidone, PVP) construct monodisperse water-stable Pt nanoclusters (Pt NCs) for enhanced immunoassay cardiac troponin I (cTnI). The PVP-modified NC nanozyme exhibited up 16.3 U mg-1 peroxidase-mimicking activity, which was mainly attributed ligand modification on surface and electron-absorbing effect NCs. NCs a lower OH-transition potential, as determined by density functional theory. Under optimized experimental conditions, strategy ultrawide dynamic response range 0.005-50 ng mL-1 cTnI targets with detection limit 1.3 pg mL-1, far superior some reported test protocols. This work provides designable pathway design artificial enzymes high activity further expand practical enzyme alternatives.

Language: Английский

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Colorimetric Sensors for Chemical and Biological Sensing Applications

Sensors, Journal Year: 2023, Volume and Issue: 23(5), P. 2749 - 2749

Published: March 2, 2023

Colorimetric sensors have been widely used to detect numerous analytes due their cost-effectiveness, high sensitivity and specificity, clear visibility, even with the naked eye. In recent years, emergence of advanced nanomaterials has greatly improved development colorimetric sensors. This review focuses on (from years 2015 2022) advances in design, fabrication, applications First, classification sensing mechanisms are briefly described, design based several typical nanomaterials, including graphene its derivatives, metal oxide nanoparticles, DNA quantum dots, some other materials discussed. Then applications, especially for detection metallic non-metallic ions, proteins, small molecules, gas, virus bacteria, DNA/RNA summarized. Finally, remaining challenges future trends also

Language: Английский

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Bioinspired porous three-coordinated single-atom Fe nanozyme with oxidase-like activity for tumor visual identification via glutathione

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 6, 2023

Inspired by structures of natural metalloenzymes, a biomimetic synthetic strategy is developed for scalable synthesis porous Fe-N3 single atom nanozymes (pFeSAN) using hemoglobin as Fe-source and template. pFeSAN delivers 3.3- 8791-fold higher oxidase-like activity than Fe-N4 Fe3O4 nanozymes. The high catalytic performance attributed to (1) the suppressed aggregation atomically dispersed Fe; (2) facilitated mass transfer maximized exposure active sites created mesopores thermal removal (2 ~ 3 nm); (3) unique electronic configuration oxygen-to-water oxidation pathway (analogy with cytochrome c oxidase). successfully demonstrated rapid colorimetric detection glutathione low limit (2.4 nM) wide range (50 nM-1 mM), further real-time, facile, (~6 min) precise visualization analysis methodology tumors via level, showing its potentials diagnostic clinic applications.

Language: Английский

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The Nanozyme Revolution: Enhancing the Performance of Medical Biosensing Platforms

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(10)

Published: April 27, 2023

Nanozymes represent a class of nanosized materials that exhibit innate catalytic properties similar to biological enzymes. The unique features these have positioned them as promising candidates for applications in clinical sensing devices, specifically those employed at the point-of-care. They notably found use means amplify signals nanosensor-based platforms and thereby improve sensor detection limits. Recent developments understanding fundamental chemistries underpinning enabled development highly effective nanozymes capable clinically relevant biomarkers limits compete with "gold-standard" techniques. However, there remain considerable hurdles need be overcome before nanozyme-based sensors can utilized platform ready use. An overview current understandings disease diagnostics biosensing unmet challenges must considered prior their translation diagnostic tests is provided.

Language: Английский

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Research progress of nanozymes in colorimetric biosensing: Classification, activity and application

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 487, P. 150612 - 150612

Published: March 21, 2024

Language: Английский

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Transition-Metal-Based Nanozymes: Synthesis, Mechanisms of Therapeutic Action, and Applications in Cancer Treatment

ACS Nano, Journal Year: 2024, Volume and Issue: 18(19), P. 12049 - 12095

Published: May 2, 2024

Cancer, as one of the leading causes death worldwide, drives advancement cutting-edge technologies for cancer treatment. Transition-metal-based nanozymes emerge promising therapeutic nanodrugs that provide a reference therapy. In this review, we present recent breakthrough First, comprehensively outline preparation strategies involved in creating transition-metal-based nanozymes, including hydrothermal method, solvothermal chemical reduction biomimetic mineralization and sol–gel method. Subsequently, elucidate catalytic mechanisms (catalase (CAT)-like activities), peroxidase (POD)-like oxidase (OXD)-like activities) superoxide dismutase (SOD)-like along with their activity regulation such morphology control, size manipulation, modulation, composition adjustment surface modification under environmental stimulation. Furthermore, elaborate on diverse applications anticancer therapies encompassing radiotherapy (RT), chemodynamic therapy (CDT), photodynamic (PDT), photothermal (PTT), sonodynamic (SDT), immunotherapy, synergistic Finally, challenges faced by are discussed alongside future research directions. The purpose review is to offer scientific guidance will enhance clinical based transition metals.

Language: Английский

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Nanozyme Inhibited Sensor Array for Biothiol Detection and Disease Discrimination Based on Metal Ion-Doped Carbon Dots

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(23), P. 8906 - 8913

Published: June 2, 2023

Developing highly active and sensitive nanozymes for biothiol analysis is of vital significance due to their essential roles in disease diagnosis. Herein, two metal ion-doped carbon dots (M-CDs) with high peroxidase-like activity were designed prepared detection identification through the colorimetric sensor array technique. The M-CDs can strongly catalyze decomposition H2O2, accompanied by color changes 3,3′,5,5′-tetramethylbenzidine (TMB) from colorless blue, indicating peroxidase-mimicking activities M-CDs. Compared pure (CDs), exhibited enhanced owing synergistic effect between ions CDs. However, strong binding affinity biothiols ions, catalytic could be inhibited different diverse degrees. Therefore, using TMB as a chromogenic substrate presence developed form differential fingerprints three most important (i.e., cysteine (Cys), homocysteine (Hcy), glutathione (GSH)), which accurately discriminated pattern recognition methods hierarchical clustering (HCA) principal component (PCA)) limit 5 nM. Moreover, single various concentrations mixtures was also realized. Furthermore, actual samples such cells sera well distinguished as-fabricated array, demonstrating its potential

Language: Английский

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Bimetallic Single-Atom Nanozyme-Based Electrochemical-Photothermal Dual-Function Portable Immunoassay with Smartphone Imaging

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(33), P. 13663 - 13671

Published: Aug. 10, 2024

Rapid and accurate detection of human epidermal growth factor receptor 2 (HER2) is crucial for the early diagnosis prognosis breast cancer. In this study, we reported an iron-manganese ion N-doped carbon single-atom catalyst (FeMn-NC

Language: Английский

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Spherical Hydrogel Sensor Based on PB@Fe-COF@Au Nanoparticles with Triplet Peroxidase-like Activity and Multiple Capture Sites for Effective Detection of Organophosphorus Pesticides

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(5), P. 6473 - 6485

Published: Jan. 31, 2023

The residues of organophosphorus pesticides (OPs) have drawn worldwide increasing attention because their potential fatal effects on human health and ecological systems. It is great significance to develop an efficient portable method for in-field detection OPs. Herein, a novel core-shell nanocomposite prussian blue@Fe-covalent organic framework@Au (PB@Fe-COF@Au) was constructed. Fe2+ Fe3+ in PB nanoparticle (PBNP) cores, Fe-porphyrin COF shells, AuNPs grown shells all acted as peroxidase-like catalytic active sites, enabling PB@Fe-COF@Au possess triplet activity. A colorimetric, affordable, sensitive, selective strategy designed detect Compared with previous reports, this sensor realized wider linear range chlorpyrifos 10-800 ng mL-1 relatively lower limit 0.61 mL-1, which attributed the overlapping triple sites response assay successfully employed food environmental samples. Moreover, meet demand OPs, spherical hydrogel based visual, portable, equipment-free features fabricated. This work provides new pathway design apply effective nanozymes on-site monitoring pesticides.

Language: Английский

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A colorimetric and photothermal dual-mode biosensing platform based on nanozyme-functionalized flower-like DNA structures for tumor-derived exosome detection

Talanta, Journal Year: 2023, Volume and Issue: 258, P. 124456 - 124456

Published: March 15, 2023

Language: Английский

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