Talanta, Год журнала: 2024, Номер 272, С. 125838 - 125838
Опубликована: Фев. 27, 2024
Язык: Английский
Cells, Год журнала: 2023, Номер 12(10), С. 1416 - 1416
Опубликована: Май 17, 2023
Extracellular vesicles (EVs) such as ectosomes and exosomes have gained attention promising natural carriers for drug delivery. Exosomes, which range from 30 to 100 nm in diameter, possess a lipid bilayer are secreted by various cells. Due their high biocompatibility, stability, low immunogenicity, favored cargo carriers. The membrane of also offers protection against degradation, making them desirable candidate However, loading into remains be challenge. Despite strategies incubation, electroporation, sonication, extrusion, freeze–thaw cycling, transfection that been developed facilitate loading, inadequate efficiency still persists. This review an overview current delivery using summarizes recent approaches small-molecule, nucleic acid, protein drugs exosomes. With insights these studies, we provide ideas more efficient effective molecules
Язык: Английский
Процитировано
160
Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 10
Опубликована: Янв. 13, 2023
Exosomes are the smallest extracellular vesicles that can be released by practically all cell types, and range in size from 30 nm to 150 nm. As major marker of liquid biopsies, exosomes have great potential for disease diagnosis, therapy, prognosis. However, their inherent heterogeneity, complexity biological fluids, presence nanoscale contaminants make isolation a challenge. Traditional methods cumbersome challenging with complex time-consuming operations. In recent years, emergence microfluidic chips, nanolithography, electro-deposition, other technologies has promoted combination innovation methods. The application these brought very considerable benefits such as ultra-fast, portable integration, low loss. There significant functional improvements yield, purity, clinical applications. this review, series summarized, emphasis on emerging methods, in-depth comparison analysis each method provided, including principles, merits, demerits.
Язык: Английский
Процитировано
112
Chemical Reviews, Год журнала: 2024, Номер 124(3), С. 929 - 1033
Опубликована: Янв. 29, 2024
RNA-based therapies have catalyzed a revolutionary transformation in the biomedical landscape, offering unprecedented potential disease prevention and treatment. However, despite their remarkable achievements, these encounter substantial challenges including low stability, susceptibility to degradation by nucleases, prominent negative charge, thereby hindering further development. Chemically modified platforms emerged as strategic innovation, focusing on precise alterations either RNA moieties or associated delivery vectors. This comprehensive review delves into platforms, underscoring significance augmenting performance translational prospects of therapeutics. It encompasses an in-depth analysis various chemically that been instrumental propelling therapeutics toward clinical utility. Moreover, scrutinizes rationale behind diverse chemical modification techniques aiming at optimizing therapeutic efficacy molecules, facilitating robust management. Recent empirical studies corroborating enhancement through modifications are highlighted. Conclusively, we offer profound insights transformative impact drugs delineates prospective trajectories for future development integration.
Язык: Английский
Процитировано
53
CNS Neuroscience & Therapeutics, Год журнала: 2024, Номер 30(3)
Опубликована: Март 1, 2024
Abstract Background Exosomes are vesicles secreted by all types of mammalian cells. They characterized a double‐layered lipid membrane structure. serve as carriers for plethora signal molecules, including DNA, RNA, proteins, and lipids. Their unique capability effortlessly crossing the blood–brain barrier underscores their critical role in progression various neurological disorders. This includes, but is not limited to, diseases such Alzheimer's, Parkinson's, ischemic stroke. Establishing stable mature methods isolating exosomes prerequisite study biomedical significance. The extraction technologies include differential centrifugation, density gradient size exclusion chromatography, ultrafiltration, polymer coprecipitation, immunoaffinity capture, microfluidic, so forth. Each technology has its own advantages disadvantages, standards have been unified internationally. Aims review aimed to showcase recent advancements exosome isolation techniques thoroughly compare disadvantages different methods. Furthermore, significant research progress made using diagnosing treating central nervous system (CNS) emphasized. Conclusion varying result differences concentration, purity, exosomes. efficient separation facilitates widespread application, particularly diagnosis treatment CNS diseases.
Язык: Английский
Процитировано
21
Molecular Cancer, Год журнала: 2025, Номер 24(1)
Опубликована: Фев. 14, 2025
Extracellular vesicles (EVs), nanoscale secreted by cells, have attracted considerable attention in recent years due to their role tumor immunomodulation. These facilitate intercellular communication transporting proteins, nucleic acids, and other biologically active substances, they exhibit a dual development immune evasion mechanisms. Specifically, EVs can assist cells evading surveillance attack impairing cell function or modulating immunosuppressive pathways, thereby promoting progression metastasis. Conversely, also transport release immunomodulatory factors that stimulate the activation regulation of system, enhancing body's capacity combat malignant diseases. This functionality presents promising avenues targets for immunotherapy. By examining biological characteristics influence on immunity, novel therapeutic strategies be developed improve efficacy relevance cancer treatment. review delineates complex immunomodulation explores potential implications approaches, aiming establish theoretical foundation provide practical insights advancement future EVs-based immunotherapy strategies.
Язык: Английский
Процитировано
6
Lab on a Chip, Год журнала: 2022, Номер 23(1), С. 44 - 61
Опубликована: Окт. 27, 2022
In the field of cancer detection, technologies to analyze tumors using biomarkers circulating in fluids such as blood have developed rapidly based on liquid biopsy.
Язык: Английский
Процитировано
62
Analytical Chemistry, Год журнала: 2023, Номер 95(44), С. 16029 - 16048
Опубликована: Окт. 24, 2023
Extracellular vesicles (EVs) are crucial mediators of intercellular communication and can be classified based on their physical properties, biomolecular structure, origin. Among EVs, exosomes have garnered significant attention due to potential as therapeutic diagnostic tools. Exosomes released via fusion multivesicular bodies plasma membranes isolated from various biofluids using methods such differential ultracentrifugation, immune affinity capture, ultrafiltration, size exclusion chromatography. Herein, an overview different techniques for exosome characterization isolation, well the diverse applications detection, including use in drug delivery disease diagnosis, is provided. Additionally, we discuss emerging field detection by sensors, which offers up-and-coming avenue point-of-care tools development. Overall, this review aims provide a exhaustive up-to-date summary current state research.
Язык: Английский
Процитировано
39
Journal of Pharmaceutical Analysis, Год журнала: 2023, Номер 13(12), С. 1429 - 1451
Опубликована: Авг. 24, 2023
With the continuous discovery and research of predictive cancer-related biomarkers, liquid biopsy shows great potential in cancer diagnosis. Surface-enhanced Raman scattering (SERS) microfluidic technology have received much attention among various biomarker detection methods. The former has ultrahigh sensitivity can provide a unique fingerprint. In contrast, latter characteristics miniaturization integration, which realize accurate control samples high-throughput through design. Both for point-of-care testing (POCT), their combination (lab-on-a-chip SERS (LoC-SERS)) good compatibility. this paper, basic situation circulating proteins, tumor cells, exosomes, DNA (ctDNA), microRNA (miRNA) diagnosis cancers is reviewed, these biomarkers by LoC-SERS platform recent years described detail. At same time, challenges future development are discussed at end review. Summarizing current expected to reference scholars engaged related work interested field.
Язык: Английский
Процитировано
35
Lab on a Chip, Год журнала: 2023, Номер 23(13), С. 2922 - 2941
Опубликована: Янв. 1, 2023
We provide a comprehensive review of the strategies for integrating separation and detection units in microfluidic assays liquid biopsy.
Язык: Английский
Процитировано
27
Clinica Chimica Acta, Год журнала: 2024, Номер 565, С. 119983 - 119983
Опубликована: Окт. 3, 2024
Язык: Английский
Процитировано
16