Talanta, Journal Year: 2023, Volume and Issue: 267, P. 125188 - 125188
Published: Sept. 13, 2023
Language: Английский
Cells, Journal Year: 2023, Volume and Issue: 12(10), P. 1416 - 1416
Published: May 17, 2023
Extracellular vesicles (EVs) such as ectosomes and exosomes have gained attention promising natural carriers for drug delivery. Exosomes, which range from 30 to 100 nm in diameter, possess a lipid bilayer are secreted by various cells. Due their high biocompatibility, stability, low immunogenicity, favored cargo carriers. The membrane of also offers protection against degradation, making them desirable candidate However, loading into remains be challenge. Despite strategies incubation, electroporation, sonication, extrusion, freeze–thaw cycling, transfection that been developed facilitate loading, inadequate efficiency still persists. This review an overview current delivery using summarizes recent approaches small-molecule, nucleic acid, protein drugs exosomes. With insights these studies, we provide ideas more efficient effective molecules
Language: Английский
Citations
154
Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 10
Published: Jan. 13, 2023
Exosomes are the smallest extracellular vesicles that can be released by practically all cell types, and range in size from 30 nm to 150 nm. As major marker of liquid biopsies, exosomes have great potential for disease diagnosis, therapy, prognosis. However, their inherent heterogeneity, complexity biological fluids, presence nanoscale contaminants make isolation a challenge. Traditional methods cumbersome challenging with complex time-consuming operations. In recent years, emergence microfluidic chips, nanolithography, electro-deposition, other technologies has promoted combination innovation methods. The application these brought very considerable benefits such as ultra-fast, portable integration, low loss. There significant functional improvements yield, purity, clinical applications. this review, series summarized, emphasis on emerging methods, in-depth comparison analysis each method provided, including principles, merits, demerits.
Language: Английский
Citations
106
Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(3), P. 929 - 1033
Published: Jan. 29, 2024
RNA-based therapies have catalyzed a revolutionary transformation in the biomedical landscape, offering unprecedented potential disease prevention and treatment. However, despite their remarkable achievements, these encounter substantial challenges including low stability, susceptibility to degradation by nucleases, prominent negative charge, thereby hindering further development. Chemically modified platforms emerged as strategic innovation, focusing on precise alterations either RNA moieties or associated delivery vectors. This comprehensive review delves into platforms, underscoring significance augmenting performance translational prospects of therapeutics. It encompasses an in-depth analysis various chemically that been instrumental propelling therapeutics toward clinical utility. Moreover, scrutinizes rationale behind diverse chemical modification techniques aiming at optimizing therapeutic efficacy molecules, facilitating robust management. Recent empirical studies corroborating enhancement through modifications are highlighted. Conclusively, we offer profound insights transformative impact drugs delineates prospective trajectories for future development integration.
Language: Английский
Citations
51
Lab on a Chip, Journal Year: 2022, Volume and Issue: 23(1), P. 44 - 61
Published: Oct. 27, 2022
In the field of cancer detection, technologies to analyze tumors using biomarkers circulating in fluids such as blood have developed rapidly based on liquid biopsy.
Language: Английский
Citations
57
Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(44), P. 16029 - 16048
Published: Oct. 24, 2023
Extracellular vesicles (EVs) are crucial mediators of intercellular communication and can be classified based on their physical properties, biomolecular structure, origin. Among EVs, exosomes have garnered significant attention due to potential as therapeutic diagnostic tools. Exosomes released via fusion multivesicular bodies plasma membranes isolated from various biofluids using methods such differential ultracentrifugation, immune affinity capture, ultrafiltration, size exclusion chromatography. Herein, an overview different techniques for exosome characterization isolation, well the diverse applications detection, including use in drug delivery disease diagnosis, is provided. Additionally, we discuss emerging field detection by sensors, which offers up-and-coming avenue point-of-care tools development. Overall, this review aims provide a exhaustive up-to-date summary current state research.
Language: Английский
Citations
36
Journal of Pharmaceutical Analysis, Journal Year: 2023, Volume and Issue: 13(12), P. 1429 - 1451
Published: Aug. 24, 2023
With the continuous discovery and research of predictive cancer-related biomarkers, liquid biopsy shows great potential in cancer diagnosis. Surface-enhanced Raman scattering (SERS) microfluidic technology have received much attention among various biomarker detection methods. The former has ultrahigh sensitivity can provide a unique fingerprint. In contrast, latter characteristics miniaturization integration, which realize accurate control samples high-throughput through design. Both for point-of-care testing (POCT), their combination (lab-on-a-chip SERS (LoC-SERS)) good compatibility. this paper, basic situation circulating proteins, tumor cells, exosomes, DNA (ctDNA), microRNA (miRNA) diagnosis cancers is reviewed, these biomarkers by LoC-SERS platform recent years described detail. At same time, challenges future development are discussed at end review. Summarizing current expected to reference scholars engaged related work interested field.
Language: Английский
Citations
33
Lab on a Chip, Journal Year: 2023, Volume and Issue: 23(13), P. 2922 - 2941
Published: Jan. 1, 2023
We provide a comprehensive review of the strategies for integrating separation and detection units in microfluidic assays liquid biopsy.
Language: Английский
Citations
26
CNS Neuroscience & Therapeutics, Journal Year: 2024, Volume and Issue: 30(3)
Published: March 1, 2024
Abstract Background Exosomes are vesicles secreted by all types of mammalian cells. They characterized a double‐layered lipid membrane structure. serve as carriers for plethora signal molecules, including DNA, RNA, proteins, and lipids. Their unique capability effortlessly crossing the blood–brain barrier underscores their critical role in progression various neurological disorders. This includes, but is not limited to, diseases such Alzheimer's, Parkinson's, ischemic stroke. Establishing stable mature methods isolating exosomes prerequisite study biomedical significance. The extraction technologies include differential centrifugation, density gradient size exclusion chromatography, ultrafiltration, polymer coprecipitation, immunoaffinity capture, microfluidic, so forth. Each technology has its own advantages disadvantages, standards have been unified internationally. Aims review aimed to showcase recent advancements exosome isolation techniques thoroughly compare disadvantages different methods. Furthermore, significant research progress made using diagnosing treating central nervous system (CNS) emphasized. Conclusion varying result differences concentration, purity, exosomes. efficient separation facilitates widespread application, particularly diagnosis treatment CNS diseases.
Language: Английский
Citations
16
Clinica Chimica Acta, Journal Year: 2024, Volume and Issue: 565, P. 119983 - 119983
Published: Oct. 3, 2024
Language: Английский
Citations
15
Clinica Chimica Acta, Journal Year: 2024, Volume and Issue: 554, P. 117757 - 117757
Published: Jan. 4, 2024
Language: Английский
Citations
14