Coordination Chemistry Reviews, Год журнала: 2024, Номер 518, С. 216081 - 216081
Опубликована: Июль 16, 2024
Язык: Английский
Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(37), С. 13577 - 13594
Опубликована: Сен. 1, 2023
Non-nucleic acid targets have posed a serious challenge to food safety. The detection of non-nucleic can enable us monitor contamination in timely manner. In recent years, the CRISPR/Cas system has been extensively explored biosensing. However, there is lack summary CRISPR/Cas-powered tailored involved This review comprehensively summarizes advances on construction and promising applications field safety related targets. current challenges futuristic perspectives are also proposed accordingly. rapidly evolving provided powerful propellant for target via integration with aptamer and/or DNAzyme. Compared traditional analytical methods, conceptually novel, essentially eliminates dependence large instruments, demonstrates capability rapid, accurate, sensitive, on-site testing.
Язык: Английский
Процитировано
34
TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 167, С. 117269 - 117269
Опубликована: Сен. 7, 2023
Язык: Английский
Процитировано
31
Deleted Journal, Год журнала: 2024, Номер 2(1)
Опубликована: Янв. 1, 2024
Abstract Paper‐based devices have attracted considerable attention in point‐of‐care testing (POCT) due to their simple operation and portability. The performance of paper‐based POC assays is further enhanced by coupling with functional nucleic acids (FNAs), which are able selectively recognize bind targets, amplify transduce signals. Several high‐performance been developed, resulting from the different spatial structures device detection strategies using FNAs. In this review, we first introduce FNAs devices, including concepts, classifications advances. following section focuses on application these taking into account one‐, two‐ three‐dimensional structures. Finally, challenges perspectives POCT discussed.
Язык: Английский
Процитировано
18
TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 172, С. 117594 - 117594
Опубликована: Фев. 8, 2024
The precision and versatility of CRISPR-based techniques, combined with the advantages nucleic acid-based nanotechnology, hold great promise in transforming landscape molecular diagnostics. While significant progress has been made, current platforms primarly focus on acid detection. To expand applicability fully leverage offered by diagnostics, ongoing efforts explore strategies to develop CRISPR sensors capable detecting a diverse range analytes beyond acids. In addition, challenges still persist adaptation for point-of-care (POC) applications, involving concerns such as portability automation, well complexities associated multiplexing. Here, we provide detailed classification comprehensive discussion facilitating conversion non-nucleic target binding into CRISPR-powered outputs an emphasis their corresponding design principles. Furthermore, second part review outlines potential solutions seamlessly integrating these user-friendly rapid tests specifically tailored (POC).
Язык: Английский
Процитировано
18
Microbial Pathogenesis, Год журнала: 2025, Номер unknown, С. 107447 - 107447
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
2
Biosensors, Год журнала: 2022, Номер 13(1), С. 39 - 39
Опубликована: Дек. 27, 2022
An aptamer is a synthetic oligonucleotide, referring to single-stranded deoxyribonucleic acid or ribonucleic ligand produced by synthesis from outside the body using systematic evolution of ligands exponential enrichment (SELEX) technology. Owing their special screening process and adjustable tertiary structures, aptamers can bind multiple targets (small molecules, proteins, even whole cells) with high specificity affinity. Moreover, due simple preparation stable modification, they have been widely used construct biosensors for target detection. The paper-based sensor product low price, short detection time, operation, other superior characteristics, as rapid method. This review mainly focuses on methods aptamers, devices, applicable sensing strategies. Furthermore, design aptamer-based lateral flow assay (LFA), which underlies most promising devices commercialization, emphasized. In addition, development prospects potential applications recognition molecules are also discussed.
Язык: Английский
Процитировано
30
Microchemical Journal, Год журнала: 2025, Номер unknown, С. 112638 - 112638
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
1
International Journal of Biological Macromolecules, Год журнала: 2025, Номер 303, С. 140675 - 140675
Опубликована: Фев. 4, 2025
Язык: Английский
Процитировано
1
Journal of Nanobiotechnology, Год журнала: 2023, Номер 21(1)
Опубликована: Июль 22, 2023
The rapid and accurate identification of methicillin-resistant Staphylococcus aureus at an early antibiotic therapy stage would be benefit to disease diagnosis selection. Herein, we integrated cross-priming amplification (CPA) CRISPR/Cas 12a (designated as CPA-Cas 12a) systems establish a sensitive efficient lateral flow assay detect aureus. This relies on the CPA isothermal nucleic acid strategy which can amplify DNA extracted from accompanying indiscriminately trans-cleavage process Cas 12a/CrRNA duplex after recognizing specific sequence. Taking advantage reporter high turnover activity, dramatic change in response was achieved produce significant increase analytical sensitivity. signal conversion output were realized using strip achieve field-deployable detection. Furthermore, this bioassay accommodated with microfluidic device realize automatically portable proposed completed within 30 min detection limit 5 CFU mL-1, verified by testing bacterial suspension 202 clinical samples. Given sensitivity, specificity efficiency, colorimetric readout through could further promoted diagnosis, medication multidrug-resistant bacteria.
Язык: Английский
Процитировано
15
Sensors and Actuators B Chemical, Год журнала: 2024, Номер 408, С. 135543 - 135543
Опубликована: Март 4, 2024
Язык: Английский
Процитировано
6