Nanomaterials-Integrated CRISPR/Cas Systems: Expanding the Toolbox for Optical Detection DOI
Tianying Sun, Weiwei He, Xiangmei Chen

и другие.

ACS Sensors, Год журнала: 2025, Номер unknown

Опубликована: Апрель 9, 2025

Nanomaterials-integrated CRISPR/Cas systems have rapidly emerged as powerful next-generation platforms for optical biosensing. These integrated harness the precision of CRISPR/Cas-mediated nucleic acid detection while leveraging unique properties nanomaterials to achieve enhanced sensitivity and expanded analytical capabilities, thereby broadening their diagnostic potential. By incorporating a diverse range nanomaterials, these effectively expand toolbox detection, offering adaptable solutions tailored various challenges. This review provides comprehensive overview successfully into CRISPR/Cas-based sensing systems. It examines multiple modalities, including fluorescence, electrochemiluminescence, colorimetry, surface-enhanced Raman spectroscopy, highlighting how facilitate signal amplification, enable multiplexing, support development point-of-care applications. Additionally, practical applications in critical fields such healthcare diagnostics environmental monitoring are showcased. While offer considerable advantages, several real-world challenges complexity assay workflows, impact cost, regulatory hurdles must be addressed before widespread implementation can achieved. identifying obstacles proposing strategic solutions, we aim pave way continued advancement adoption nanomaterial-integrated biosensing technologies.

Язык: Английский

CRISPR/Cas-based nucleic acid detection strategies: Trends and challenges DOI Creative Commons
Jian Zhou, Zhuo Li, Joshua Seun Olajide

и другие.

Heliyon, Год журнала: 2024, Номер 10(4), С. e26179 - e26179

Опубликована: Фев. 1, 2024

CRISPR/Cas systems have become integral parts of nucleic acid detection apparatus and biosensors. Various such as CRISPR/Cas9, CRISPR/Cas12, CRISPR/Cas13, CRISPR/Cas14 CRISPR/Cas3 utilize different mechanisms to detect or differentiate biological activities nucleotide sequences. Usually, CRISPR/Cas-based are combined with polymerase chain reaction, loop-mediated isothermal amplification, recombinase amplification transcriptional technologies for effective diagnostics. Premised on these, many biosensors been developed acids viral bacterial pathogens in clinical samples, well other applications life sciences including biosecurity, food safety environmental assessment. Additionally, showed better specificity compared molecular diagnostic methods. In this review, we give an overview various methods highlight some advances their development components. We also discourse operational challenges advantages disadvantages systems. Finally, important considerations offered the improvement testing.

Язык: Английский

Процитировано

27

CRISPR/Cas-Powered Amplification-Free Detection of Nucleic Acids: Current State of the Art, Challenges, and Futuristic Perspectives DOI
Yaru Li, Yajie Liu, Xiaoqin Tang

и другие.

ACS Sensors, Год журнала: 2023, Номер 8(12), С. 4420 - 4441

Опубликована: Ноя. 18, 2023

CRISPR/Cas system is becoming an increasingly influential technology that has been repositioned in nucleic acid detection. A preamplification step usually required to improve the sensitivity of CRISPR/Cas-based The striking biological features CRISPR/Cas, including programmability, high and sequence specificity, single-base resolution. More strikingly, target-activated trans-cleavage could act as a biocatalytic signal transductor amplifier, thereby empowering it potentially perform detection without step. reports such work are on rise, which not only scientifically significant but also promising for futuristic end-user applications. This review started with introduction methods acids diagnostics (CRISPR-Dx). Next, we objectively discussed pros cons steps CRISPR-Dx. We then illustrated highlighted recently developed strategies CRISPR/Cas-powered amplification-free can be realized through uses ultralocalized reactors, cascade reactions, ultrasensitive systems, or others. Lastly, challenges perspectives were proposed. It expected this makes researchers better understand current emerging field, provides insight designing novel CRISPR-Dx win practicable use near future.

Язык: Английский

Процитировано

38

Argonaute-DNAzyme tandem biosensing for highly sensitive and simultaneous dual-gene detection of methicillin-resistant Staphylococcus aureus DOI
Yaru Li, Xiaoqin Tang,

Nan Wang

и другие.

Biosensors and Bioelectronics, Год журнала: 2023, Номер 244, С. 115758 - 115758

Опубликована: Окт. 26, 2023

Язык: Английский

Процитировано

30

An Argonaute-mediated bio-barcode bioassay for one-tube and on-site detection of Staphylococcus aureus DOI
Yaru Li, Yajie Liu, Qiang Zhang

и другие.

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 410, С. 135713 - 135713

Опубликована: Март 27, 2024

Язык: Английский

Процитировано

9

Multiple gRNAs-assisted CRISPR/Cas12a-based portable aptasensor enabling glucometer readout for amplification-free and quantitative detection of malathion DOI

Yi Tian,

Jiaxuan Chen,

Feng‐Zheng Chen

и другие.

Analytica Chimica Acta, Год журнала: 2025, Номер 1341, С. 343662 - 343662

Опубликована: Янв. 13, 2025

Язык: Английский

Процитировано

1

A Wireless, CRISPR-Polymer Dot Electrochemical Sensor for the Diagnosis of Bacterial Pneumonia and Multi-Drug Resistance DOI
San Hae Im, Akhmad Irhas Robby,

Heewon Choi

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(5), С. 5637 - 5647

Опубликована: Янв. 26, 2024

Rapid and accurate diagnosis is crucial for managing the global health threat posed by multidrug-resistant bacterial infections; however, current methods have limitations in either being time-consuming, labor-intensive, or requiring instruments with high costs. Addressing these challenges, we introduce a wireless electrochemical sensor integrating CRISPR/Cas system electroconductive polymer dot (PD) nanoparticles to rapidly detect pathogens from human sputum. To enhance properties, synthesized copper-ion-immobilized PD (PD-Cu), followed conjugation of deactivated Cas9 protein (dCas9) onto PD-Cu-coated Si electrodes generate dCas9-PD-Cu sensor. The integrated isothermal amplification can specifically target nucleic acids bacteria, such as antibiotic resistance genes kpc-2 mecA. exhibits sensitivity, allowing detection ∼54 femtograms acids, based on measuring changes resistivity through capture dCas9. Furthermore, sensing platform was established using Bluetooth module microcontroller unit smartphone. We demonstrate feasibility diagnosing pneumonia patients' sputum samples, achieving 92% accuracy. study presents versatile biosensor that overcome conventional diagnostics clinic.

Язык: Английский

Процитировано

9

CRISPR-based nucleic acid assays for food authentication DOI
Ruijie Deng, Lin Xu, Yong Zhang

и другие.

Trends in Food Science & Technology, Год журнала: 2024, Номер 145, С. 104351 - 104351

Опубликована: Янв. 28, 2024

Язык: Английский

Процитировано

9

Seizing the Hidden Assassin: Current Detection Strategies for Staphylococcus aureus and Methicillin-Resistant S. aureus DOI
Qi Wang, Qingli Yang

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(30), С. 16569 - 16582

Опубликована: Июль 20, 2024

(

Процитировано

7

Layer-by-layer (LBL) self-assembly efficient immobilization of glucose oxidase onto PDMS microfluidic chip towards glucose biosensing DOI

Kemeng Zhou,

Yaoyao Yu,

Zhihua Wang

и другие.

Journal of Polymer Research, Год журнала: 2025, Номер 32(1)

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1

Argonaute-Based Nucleic Acid Detection Technology: Advantages, Current Status, Challenges, and Perspectives DOI
Yaru Li, Lu Zhao,

Jiali Wang

и другие.

ACS Sensors, Год журнала: 2024, Номер 9(11), С. 5665 - 5682

Опубликована: Ноя. 11, 2024

Rapid and accurate detection is a prerequisite for precise clinical diagnostics, ensuring food safety, facilitating biotechnological applications. The Argonaute system, as cutting-edge technique, has been successfully repurposed in biosensing beyond the CRISPR/Cas system (clustered regularly interspaced short palindromic repeats CRISPR-associated proteins), which extensively researched, but recognition of PAM sequences remains restricted. Argonaute, programmable target-activated nuclease, fabricating novel methods due to its unparalleled biological features. In this comprehensive review, we initially elaborate on current nucleic acid testing nucleases, followed by delving into structure nuclease activity system. advantages compared with are highlighted discussed. Furthermore, summarize applications Argonaute-based provide an in-depth analysis future perspectives challenges. Recent research demonstrated that innovative rapidly advancing technology can overcome limitations existing potentially replace them. summary, implementation integration other technologies hold promise developing customized intelligent across various aspects.

Язык: Английский

Процитировано

6