Nanomaterials-integrated
CRISPR/Cas
systems
have
rapidly
emerged
as
powerful
next-generation
platforms
for
optical
biosensing.
These
integrated
harness
the
precision
of
CRISPR/Cas-mediated
nucleic
acid
detection
while
leveraging
unique
properties
nanomaterials
to
achieve
enhanced
sensitivity
and
expanded
analytical
capabilities,
thereby
broadening
their
diagnostic
potential.
By
incorporating
a
diverse
range
nanomaterials,
these
effectively
expand
toolbox
detection,
offering
adaptable
solutions
tailored
various
challenges.
This
review
provides
comprehensive
overview
successfully
into
CRISPR/Cas-based
sensing
systems.
It
examines
multiple
modalities,
including
fluorescence,
electrochemiluminescence,
colorimetry,
surface-enhanced
Raman
spectroscopy,
highlighting
how
facilitate
signal
amplification,
enable
multiplexing,
support
development
point-of-care
applications.
Additionally,
practical
applications
in
critical
fields
such
healthcare
diagnostics
environmental
monitoring
are
showcased.
While
offer
considerable
advantages,
several
real-world
challenges
complexity
assay
workflows,
impact
cost,
regulatory
hurdles
must
be
addressed
before
widespread
implementation
can
achieved.
identifying
obstacles
proposing
strategic
solutions,
we
aim
pave
way
continued
advancement
adoption
nanomaterial-integrated
biosensing
technologies.
Heliyon,
Год журнала:
2024,
Номер
10(4), С. e26179 - e26179
Опубликована: Фев. 1, 2024
CRISPR/Cas
systems
have
become
integral
parts
of
nucleic
acid
detection
apparatus
and
biosensors.
Various
such
as
CRISPR/Cas9,
CRISPR/Cas12,
CRISPR/Cas13,
CRISPR/Cas14
CRISPR/Cas3
utilize
different
mechanisms
to
detect
or
differentiate
biological
activities
nucleotide
sequences.
Usually,
CRISPR/Cas-based
are
combined
with
polymerase
chain
reaction,
loop-mediated
isothermal
amplification,
recombinase
amplification
transcriptional
technologies
for
effective
diagnostics.
Premised
on
these,
many
biosensors
been
developed
acids
viral
bacterial
pathogens
in
clinical
samples,
well
other
applications
life
sciences
including
biosecurity,
food
safety
environmental
assessment.
Additionally,
showed
better
specificity
compared
molecular
diagnostic
methods.
In
this
review,
we
give
an
overview
various
methods
highlight
some
advances
their
development
components.
We
also
discourse
operational
challenges
advantages
disadvantages
systems.
Finally,
important
considerations
offered
the
improvement
testing.
ACS Sensors,
Год журнала:
2023,
Номер
8(12), С. 4420 - 4441
Опубликована: Ноя. 18, 2023
CRISPR/Cas
system
is
becoming
an
increasingly
influential
technology
that
has
been
repositioned
in
nucleic
acid
detection.
A
preamplification
step
usually
required
to
improve
the
sensitivity
of
CRISPR/Cas-based
The
striking
biological
features
CRISPR/Cas,
including
programmability,
high
and
sequence
specificity,
single-base
resolution.
More
strikingly,
target-activated
trans-cleavage
could
act
as
a
biocatalytic
signal
transductor
amplifier,
thereby
empowering
it
potentially
perform
detection
without
step.
reports
such
work
are
on
rise,
which
not
only
scientifically
significant
but
also
promising
for
futuristic
end-user
applications.
This
review
started
with
introduction
methods
acids
diagnostics
(CRISPR-Dx).
Next,
we
objectively
discussed
pros
cons
steps
CRISPR-Dx.
We
then
illustrated
highlighted
recently
developed
strategies
CRISPR/Cas-powered
amplification-free
can
be
realized
through
uses
ultralocalized
reactors,
cascade
reactions,
ultrasensitive
systems,
or
others.
Lastly,
challenges
perspectives
were
proposed.
It
expected
this
makes
researchers
better
understand
current
emerging
field,
provides
insight
designing
novel
CRISPR-Dx
win
practicable
use
near
future.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(5), С. 5637 - 5647
Опубликована: Янв. 26, 2024
Rapid
and
accurate
diagnosis
is
crucial
for
managing
the
global
health
threat
posed
by
multidrug-resistant
bacterial
infections;
however,
current
methods
have
limitations
in
either
being
time-consuming,
labor-intensive,
or
requiring
instruments
with
high
costs.
Addressing
these
challenges,
we
introduce
a
wireless
electrochemical
sensor
integrating
CRISPR/Cas
system
electroconductive
polymer
dot
(PD)
nanoparticles
to
rapidly
detect
pathogens
from
human
sputum.
To
enhance
properties,
synthesized
copper-ion-immobilized
PD
(PD-Cu),
followed
conjugation
of
deactivated
Cas9
protein
(dCas9)
onto
PD-Cu-coated
Si
electrodes
generate
dCas9-PD-Cu
sensor.
The
integrated
isothermal
amplification
can
specifically
target
nucleic
acids
bacteria,
such
as
antibiotic
resistance
genes
kpc-2
mecA.
exhibits
sensitivity,
allowing
detection
∼54
femtograms
acids,
based
on
measuring
changes
resistivity
through
capture
dCas9.
Furthermore,
sensing
platform
was
established
using
Bluetooth
module
microcontroller
unit
smartphone.
We
demonstrate
feasibility
diagnosing
pneumonia
patients'
sputum
samples,
achieving
92%
accuracy.
study
presents
versatile
biosensor
that
overcome
conventional
diagnostics
clinic.
ACS Sensors,
Год журнала:
2024,
Номер
9(11), С. 5665 - 5682
Опубликована: Ноя. 11, 2024
Rapid
and
accurate
detection
is
a
prerequisite
for
precise
clinical
diagnostics,
ensuring
food
safety,
facilitating
biotechnological
applications.
The
Argonaute
system,
as
cutting-edge
technique,
has
been
successfully
repurposed
in
biosensing
beyond
the
CRISPR/Cas
system
(clustered
regularly
interspaced
short
palindromic
repeats
CRISPR-associated
proteins),
which
extensively
researched,
but
recognition
of
PAM
sequences
remains
restricted.
Argonaute,
programmable
target-activated
nuclease,
fabricating
novel
methods
due
to
its
unparalleled
biological
features.
In
this
comprehensive
review,
we
initially
elaborate
on
current
nucleic
acid
testing
nucleases,
followed
by
delving
into
structure
nuclease
activity
system.
advantages
compared
with
are
highlighted
discussed.
Furthermore,
summarize
applications
Argonaute-based
provide
an
in-depth
analysis
future
perspectives
challenges.
Recent
research
demonstrated
that
innovative
rapidly
advancing
technology
can
overcome
limitations
existing
potentially
replace
them.
summary,
implementation
integration
other
technologies
hold
promise
developing
customized
intelligent
across
various
aspects.
