Evaluation of Drug Permeation Enhancement by Using In Vitro and Ex Vivo Models DOI Creative Commons
Dewald Steyn, Anja Haasbroek‐Pheiffer, Wihan Pheiffer

и другие.

Pharmaceuticals, Год журнала: 2025, Номер 18(2), С. 195 - 195

Опубликована: Янв. 31, 2025

Drugs administered by means of extravascular routes drug administration must be absorbed into the systemic circulation, which involves movement molecules across biological barriers such as epithelial cells that cover mucosal surfaces or stratum corneum covers skin. Some drugs exhibit poor permeation membranes may experience excessive degradation during first-pass metabolism, tends to limit their bioavailability. Various strategies have been used improve Absorption enhancement include co-administration chemical enhancers, enzymes, and/or efflux transporter inhibitors, changes, and specialized dosage form designs. Models with physiological relevance are needed evaluate efficacy absorption techniques. in vitro cell culture models ex vivo tissue explored quantify effectiveness strategies. This review deliberates on use for evaluation selected including nasal, oromucosal, pulmonary, oral, rectal, transdermal administration.

Язык: Английский

From Organ-on-a-Chip to Human-on-a-Chip: A Review of Research Progress and Latest Applications DOI

Yisha Huang,

Tong Liu,

Qi Huang

и другие.

ACS Sensors, Год журнала: 2024, Номер 9(7), С. 3466 - 3488

Опубликована: Июль 11, 2024

Organ-on-a-Chip (OOC) technology, which emulates the physiological environment and functionality of human organs on a microfluidic chip, is undergoing significant technological advancements. Despite its rapid evolution, this technology also facing notable challenges, such as lack vascularization, development multiorgan-on-a-chip systems, replication body single chip. The progress has played crucial role in steering OOC toward mimicking microenvironment, including microenvironment replication, multiorgan microphysiological systems. Additionally, advancements detection, analysis, organoid imaging technologies have enhanced efficiency Organs-on-Chips (OOCs). In particular, integration artificial intelligence revolutionized imaging, significantly enhancing high-throughput drug screening. Consequently, review covers research Human-on-a-chip, sensors OOCs, latest applications biomedical field.

Язык: Английский

Процитировано

15
Patient-derived organoids in precision cancer medicine DOI Creative Commons
Le Tong, Weiyingqi Cui, Boya Zhang

и другие.

Med, Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

13
Bridging the Gap: Integrating 3D Bioprinting and Microfluidics for Advanced Multi-Organ Models in Biomedical Research DOI Creative Commons
Marco De Spirito, Valentina Palmieri, Giordano Perini

и другие.

Bioengineering, Год журнала: 2024, Номер 11(7), С. 664 - 664

Опубликована: Июнь 28, 2024

Recent advancements in 3D bioprinting and microfluidic lab-on-chip systems offer promising solutions to the limitations of traditional animal models biomedical research. Three-dimensional enables creation complex, patient-specific tissue that mimic human physiology more accurately than models. These bioprinted tissues, when integrated with systems, can replicate dynamic environment body, allowing for development multi-organ This integration facilitates precise drug screening personalized therapy by simulating interactions between different organ systems. Such innovations not only improve predictive accuracy but also address ethical concerns associated testing, aligning three Rs principle. Future directions include enhancing resolution, developing advanced bioinks, incorporating AI optimized system design. technologies hold potential revolutionize development, regenerative medicine, disease modeling, leading effective, personalized, humane treatments.

Язык: Английский

Процитировано

9
Small Intestine on a Chip Demonstrates Physiologic Mucus Secretion in the Presence of Lacticaseibacillus rhamnosus Biofilm DOI Creative Commons
Sanat Kumar Dash, Cláudia N. H. Marques, Gretchen J. Mahler

и другие.

Biotechnology and Bioengineering, Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

ABSTRACT The small intestine is an area of the digestive system difficult to access using current medical procedures, which prevents studies on interactions between food, drugs, intestinal epithelium, and resident microbiota. Therefore, there a need develop novel microfluidic models that mimic biological mechanical environments. These can be used for drug discovery disease modeling have potential reduce reliance animal models. goal this study was chip with both enterocyte (Caco‐2) goblet (HT29‐MTX) cells cocultured Lacticaseibacillus rhamnosus biofilms, one several genera present in L. introduced following establishment epithelial barrier. shear stress within device kept lower physiological range (0.3 mPa) enable biofilm development over vitro epithelium. barrier differentiated after 5 days dynamic culture cell polarity permeability similar human intestine. presence biofilms did not alter barrier's conditions. Under fluid flow, complete model remained viable functional more than days, while static only 1 day. increased secretion acidic neutral mucins by Furthermore, also showed MUC2 production, dominant gel‐forming mucin This builds previous publications as it establishes stable environment closely mimics vivo conditions physiology, food‐intestinal interactions, development.

Язык: Английский

Процитировано

1
Recent advances towards point-of-care devices for fungal detection: Emphasizing the role of plasmonic nanomaterials in current and future technologies DOI Creative Commons

Daria Stoia,

Luciano De Sio, Francesca Petronella

и другие.

Biosensors and Bioelectronics, Год журнала: 2024, Номер 255, С. 116243 - 116243

Опубликована: Март 24, 2024

Fungal infections are a significant global health problem, particularly affecting individuals with weakened immune systems. Moreover, as uncontrolled antibiotic and immunosuppressant use increases continuously, fungal have seen dramatic increase, some strains developing resistance. Traditional approaches to identifying often rely on morphological characteristics, thus owning limitations, such struggles in several or distinguishing between similar morphologies. This review explores the multifaceted impact of fungi individuals, healthcare providers, society, highlighting often-underestimated economic burden implications these infections. In light serious constraints traditional identification methods, this discusses potential plasmonic nanoparticle-based biosensors for infection identification. These can enable rapid precise pathogen detection by exploiting readout approaches, including various spectroscopic techniques, colorimetric electrochemical assays, well lateral-flow immunoassay methods. we report remarkable Lab Chip technology microfluidic devices, they recently emerged class advanced biosensors. Finally, provide an overview smartphone-based Point-of-Care devices associated technologies developed detecting pathogens.

Язык: Английский

Процитировано

9
Recent advances in Organ-on-a-Chip models: How precision engineering integrates cutting edge technologies in fabrication and characterization DOI
Sorour Sadeghzade, Mohammad Hosseini Hooshiar, Hossein Akbari

и другие.

Applied Materials Today, Год журнала: 2024, Номер 38, С. 102231 - 102231

Опубликована: Май 14, 2024

Язык: Английский

Процитировано

9
Tuneable hydrogel patterns in pillarless microfluidic devices DOI Creative Commons
Claudia Olaizola‐Rodrigo, Sujey Palma-Florez, Teodora Ranđelović

и другие.

Lab on a Chip, Год журнала: 2024, Номер 24(7), С. 2094 - 2106

Опубликована: Янв. 1, 2024

Organ-on-chip (OOC) technology has recently emerged as a powerful tool to mimic physiological or pathophysiological conditions through cell culture in microfluidic devices. One of its main goals is bypassing animal testing and encouraging more personalized medicine. The recent incorporation hydrogels 3D scaffolds into devices changed biomedical research since they provide biomimetic extracellular matrix recreate tissue architectures. However, this presents some drawbacks such the necessity for physical structures pillars confine these hydrogels, well difficulty reaching different shapes patterns create convoluted gradients realistic biological structures. In addition, can also interfere with fluid flow, altering local shear forces and, therefore, modifying mechanical environment OOC model. work, we present methodology based on plasma surface treatment that allows building chambers abutment-free capable producing precise stress distributions. Therefore, pillarless arbitrary geometries are needed obtain versatile, reliable, experimental models. Through computational simulation studies, changes demonstrated designed fabricated geometries. To prove versatility new technique, blood-brain barrier model been recreated, achieving an uninterrupted endothelial emulates part neurovascular network brain. Finally, developed could avoid limitations mentioned above, allowing development models complex adaptable geometries, cell-to-cell contact if required, where flow be controlled.

Язык: Английский

Процитировано

7
Retinal organoids with X-linked retinoschisis RS1 (E72K) mutation exhibit a photoreceptor developmental delay and are rescued by gene augmentation therapy DOI Creative Commons

Chunwen Duan,

Chengcheng Ding,

Xihao Sun

и другие.

Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)

Опубликована: Май 31, 2024

X-linked juvenile retinoschisis (XLRS) is an inherited disease caused by RS1 gene mutation, which leads to retinal splitting and visual impairment. The mechanism of RS1-associated degeneration not fully understood. Besides, animal models XLRS have limitations in the study XLRS. Here, we used human induced pluripotent stem cell (hiPSC)-derived organoids (ROs) investigate mechanisms potential treatments for

Язык: Английский

Процитировано

7
Organoids‐On‐a‐Chip for Personalized Precision Medicine DOI Open Access

Yunqi Man,

Yanfei Liu, Qi‐Wen Chen

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Окт. 13, 2024

Abstract The development of personalized precision medicine has become a pivotal focus in modern healthcare. Organoids‐on‐a‐Chip (OoCs), groundbreaking fusion organoid culture and microfluidic chip technology, emerged as promising approach to advancing patient‐specific treatment strategies. In this review, the diverse applications OoCs are explored, particularly their role medicine, potential cutting‐edge technology is highlighted. By utilizing patient‐derived organoids, offer pathway optimize treatments, create precise disease models, investigate mechanisms, conduct drug screenings, individualize therapeutic emphasis on significance technological revolutionizing healthcare improving patient outcomes. Furthermore, transformative future prospects, ongoing advancements field, with genomic multi‐omics integration, ethical frameworks discussed. convergence these innovations can empower patients, redefine approaches, shape

Язык: Английский

Процитировано

7
Organ-on-a-chip: future of female reproductive pathophysiological models DOI Creative Commons

Zhimin Deng,

Fangfang Dai, Ruiqi Wang

и другие.

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Июль 31, 2024

Abstract The female reproductive system comprises the internal and external genitalia, which communicate through intricate endocrine pathways. Besides secreting hormones that maintain secondary sexual characteristics, it also produces follicles offspring. However, in vitro systems have been very limited recapitulating specific anatomy pathophysiology of women. Organ-on-a-chip technology, based on microfluidics, can better simulate cellular microenvironment vivo, opening a new field for basic clinical research diseases. This technology not only reconstruct organ structure but emulate function as much possible. precisely controlled fluidic provided by microfluidics vividly mimics complex hormone crosstalk among various organs system, making powerful preclinical tool future pathophysiological models system. Here, we review application organ-on-a-chip platforms systems, focusing latest progress developing reproduce physiological functions or disease features tissues, highlighting challenges directions this field.

Язык: Английский

Процитировано

6