Dyes and Pigments, Год журнала: 2024, Номер unknown, С. 112581 - 112581
Опубликована: Дек. 1, 2024
Язык: Английский
Analytica Chimica Acta, Год журнала: 2025, Номер 1340, С. 343665 - 343665
Опубликована: Янв. 14, 2025
Язык: Английский
Процитировано
1
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Март 9, 2025
Neutrophil-derived extracellular vesicles (NEVs) are critically involved in disease progression and considered potential biomarkers. However, the tedious processes of NEV separation detection restrain their use. Herein, we presented an integrated microfluidic chip for (IMCN) analysis, which achieved immune-separation CD66b+ NEVs multiplexed contained miRNAs (termed signatures) by using 10 μL serum samples. The optimized microchannel flow rate IMCN enabled efficient capture (>90%). After recognition captured a specific CD63 aptamer, on-chip rolling circle amplification (RCA) reaction was triggered released aptamers from heat-lysed NEVs. Then, RCA products bound to molecular beacons (MBs), initiating allosteric hairpin structures amplified "turn on" fluorescence signals (RCA-MB assay). Clinical sample analysis showed that signatures had high area under curve (AUC) distinguishing between healthy control (HC) gastric cancer (GC) (0.891), benign diseases (BGD) GC (0.857). Notably, AUC reached 0.912 with combination five biomarkers (NEV signatures, CEA, CA199) differentiate HC, diagnostic accuracy further increased machine learning (ML)-based ensemble classification system. Therefore, developed is valuable platform may have use diagnosis.
Язык: Английский
Процитировано
1
Biosensors and Bioelectronics, Год журнала: 2024, Номер 264, С. 116671 - 116671
Опубликована: Авг. 17, 2024
Язык: Английский
Процитировано
6
International Journal of Biological Macromolecules, Год журнала: 2024, Номер 276, С. 133760 - 133760
Опубликована: Июль 14, 2024
Язык: Английский
Процитировано
5
ACS Sensors, Год журнала: 2024, Номер 9(7), С. 3616 - 3624
Опубликована: Июль 9, 2024
Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-Associated Protein (CRISPR-Cas) systems have evolved several mechanisms to specifically target foreign DNA. These properties made them attractive as biosensors. The primary drawback associated with contemporary CRISPR-Cas biosensors is their weak signaling capacity, which typically compensated for by coupling the nucleic acid amplification. An alternative strategy improve capacity engineer reporter, i.e., design new signal-generating substrates Cas proteins. Unfortunately, due reliance on custom synthesis, most of these engineered reporter are inaccessible many researchers. Herein, we investigate a substrate based fluorescein (FAM)–tetramethylrhodamine (TAMRA) Förster resonant energy-transfer (FRET) pair that functions seamless "drop-in" replacement existing reporters, without need change any other aspect CRISPR-Cas12a-based assay. readily available and employs FRET produce two signals upon cleavage Cas12a. use both in ratiometric manner provides improved assay performance decreased time-to-result CRISPR-Cas12a assays when compared traditional FAM–Black Hole Quencher (BHQ) quench-based reporter. We comprehensively characterize this better understand reasons benchmark it against current standard Finally, showcase real-world utility employ Recombinase Polymerase Amplification (RPA)–CRISPR-Cas12a DNA Endonuclease-Targeted CRISPR Trans Reporter (DETECTR) detect Human papillomavirus patient-derived samples.
Язык: Английский
Процитировано
4
Chemical Communications, Год журнала: 2024, Номер 60(78), С. 10805 - 10821
Опубликована: Янв. 1, 2024
DNAzymes, a class of single-stranded catalytic DNA with good stability, high activity, and easy synthesis, functionalization modification properties, have garnered significant interest in the realm biosensing bioimaging. Their integration fluorescent dyes or chemiluminescent moieties has led to remarkable bioimaging outcomes, while DNAzyme-based biosensors demonstrated robust sensitivity selectivity detecting metal ions, nucleic acids, proteins, enzyme activities, exosomes, bacteria microorganisms. In addition, by delivering DNAzymes into tumor cells, mRNA therein can be cleaved regulate expression corresponding which further propelled application cancer gene therapy synergistic therapy. This paper reviews strategies for screening attractive such as SELEX high-throughput sequencing, briefly describes amplification mainly include hairpin assembly (CHA), walker, hybridization chain reaction (HCR), origami, CRISPR-Cas12a, rolling circle (RCA), aptamers. applications bioimaging, biosensing, are also highlighted. Subsequently, possible challenges these practical pointed out, future research directions suggested.
Язык: Английский
Процитировано
4
Materials Today Sustainability, Год журнала: 2024, Номер unknown, С. 101057 - 101057
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
4
Analytica Chimica Acta, Год журнала: 2025, Номер unknown, С. 343712 - 343712
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118174 - 118174
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
Analytical Methods, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
C-ZIF-8 enhanced the NiO photocathode current by reducing bandgap, promoting charge transfer, and increasing load of sensitizer, achieving ultra-sensitive detection Pb 2+ .
Язык: Английский
Процитировано
0