Proteomics-on-a-Chip – Microfluidics Meets Proteomics

Biosensors and Bioelectronics, Год журнала: 2025, Номер 273, С. 117122 - 117122

Опубликована: Янв. 9, 2025

Язык: Английский

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Efficient Separation-Free and Wash-Free Immunoassay for Sensitive Detection of Biomarkers Based on the Distance-Driven Chemiluminescent Technique

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

The wash-free method represents a promising strategy for enhancing the detection efficiency of automated chemiluminescent (CL) immunoassay analyzers. Herein, novel separation-free and was developed first time based on distance-driven CL technique. In immunoassay, well-designed Au–Co metal nanoclusters (NCs) exhibited excellent peroxidase-like activity good stability, allowing efficient catalysis luminol or its analogue (ABEI)-H2O2 system even at low concentrations. Furthermore, large specific surface area NCs facilitated accommodation greater number antibodies, thereby capture antigens achieving dual amplification signal. technique relied formation sandwich-type immunocomplexes. Upon generation hydroxyl radicals superoxide anion through catalytic decomposition H2O2 by NCs, ABEI within immunocomplexes could efficiently react with these radicals, leading to significant enhancement in signals. C-reactive protein (CRP) chosen as model analyte evaluate practicability proposed immunoassay. Notably, presented high sensitivity, selectivity, reproducibility, successfully determining CRP serum samples recoveries 96.55–106.29%. Accordingly, advantage separation-free, wash-free, reliable characteristics drastically simplify operation steps enhance efficiency, which would make advances revolution traditional

Язык: Английский

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Rapid Amplification and Detection of Single‐Stranded Nucleic Acids for Point‐of‐Care Diagnosis

Small Methods, Год журнала: 2025, Номер unknown

Опубликована: Янв. 6, 2025

Abstract Nucleic acid detection plays a crucial role in various applications, including disease diagnostics, research development, food safety, and environmental health monitoring. A rapid, point‐of‐care (POC) nucleic test can greatly benefit healthcare system by providing timely diagnosis for effective treatment patient management, as well supporting diseases surveillance emerging pandemic diseases. Recent advancements acids technology have led to rapid assays single‐stranded that be integrated into simple miniaturized platforms ease of use. In this review, the study focuses on developments isothermal amplification, hybridization circuits, enzyme‐based signal reporting mechanisms, show promise POC testing. The also evaluates critical technical breakthroughs identify advantages disadvantages these methods applications.

Язык: Английский

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Quantity of cu(II) ions in a copper pot by a DNAzyme-based fluorescent sensor

Food Chemistry Molecular Sciences, Год журнала: 2025, Номер 10, С. 100249 - 100249

Опубликована: Фев. 18, 2025

Язык: Английский

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Recent Progress in DNA Biosensors for Detecting Biomarkers in Living Cells

ACS Biomaterials Science & Engineering, Год журнала: 2024, Номер 10(9), С. 5595 - 5608

Опубликована: Авг. 15, 2024

Analysis of biomarkers in living cells is crucial for deciphering the dynamics as well precise diagnosis diseases. DNA biosensors employ sequences probes to offer insights into cells, and drive progress disease drug development. In this review, we present recent advances detecting cells. The basic structural components signal output method are presented. strategies crossing cell membrane also described, including coincubation, nanocarriers, nanoelectroporation techniques. Based on biomarker categorization, detail applications small molecules, RNAs, proteins, integrated targets Furthermore, future development directions summarized encourage further research growing field.

Язык: Английский

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Aggregation-induced emission-based competitive immunoassays for “signal-on” detection of proteins with multifunctional metal–organic frameworks as signal tags

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2024, Номер 325, С. 125088 - 125088

Опубликована: Сен. 3, 2024

Язык: Английский

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Immuno‐Rolling Circle Amplification (Immuno‐RCA): Biosensing Strategies, Practical Applications, and Future Perspectives

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Сен. 10, 2024

Abstract In the rapidly evolving field of life sciences and biomedicine, detecting low‐abundance biomolecules, ultraweak biosignals presents significant challenges. This has spurred a rapid development analytical techniques aiming for increased sensitivity specificity. These advancements, including signal amplification strategies integration biorecognition events, mark transformative era in bioanalytical precision accuracy. A prominent method among these innovations is immuno‐rolling circle (immuno‐RCA) technology, which effectively combines immunoassays with via RCA. process starts when targeted biomolecule, such as protein or cell, binds to an immobilized antibody probe on substrate. The introduction circular DNA template triggers RCA, leading exponential significantly enhanced intensity, thus target molecule detectable quantifiable even at single‐molecule level. review provides overview biosensing strategy extensive practical applications immuno‐RCA biomarkers. Furthermore, it scrutinizes limitations inherent sensors sets forth expectations their future trajectory. serves valuable reference advancing various domains, diagnostics, biomarker discovery, molecular imaging.

Язык: Английский

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Engineering of a DNAzyme-based aptasensor for highly efficient ATP detection and oyster freshness evaluation

Journal of Food Composition and Analysis, Год журнала: 2024, Номер unknown, С. 107176 - 107176

Опубликована: Дек. 1, 2024

Язык: Английский

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