Anti-CRISPR Anopheles mosquitoes inhibit gene drive spread under challenging behavioural conditions in large cages

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Фев. 1, 2024

CRISPR-based gene drives have the potential to spread within populations and are considered as promising vector control tools. A doublesex-targeting drive was able suppress laboratory Anopheles mosquito in small large cages, it is for field application. Challenges related field-use of evolving regulatory framework suggest that systems modulate or revert action drives, could be part post-release risk-mitigation plans. In this study, we challenge an AcrIIA4-based anti-drive inhibit age-structured gambiae population under complex feeding behavioural conditions. stochastic model predicts experimentally-observed genotype dynamics medium-sized cages highlights necessity large-sized cage trials. These experiments experimental-modelling demonstrate effectiveness different scenarios, providing further corroboration its use controlling Anopheles.

Язык: Английский

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New self-sexing Aedes aegypti strain eliminates barriers to scalable and sustainable vector control for governments and communities in dengue-prone environments

Frontiers in Bioengineering and Biotechnology, Год журнала: 2022, Номер 10

Опубликована: Окт. 25, 2022

For more than 60 years, efforts to develop mating-based mosquito control technologies have largely failed produce solutions that are both effective and scalable, keeping them out of reach most governments communities in disease-impacted regions globally. High pest suppression levels trials yet fully translate into broad Aedes aegypti solutions. Two primary challenges date-the need for complex sex-sorting prevent female releases, cumbersome processes rearing releasing male adult mosquitoes-present significant barriers existing methods. As the host range continues advance new geographies due increasing globalisation climate change, traditional chemical-based approaches under mounting pressure from stringent regulatory ongoing development insecticide resistance. It is no exaggeration state tools, which equal parts needed now ever. This paper describes field evaluation a self-sexing strain has been designed combine targeted vector suppression, operational simplicity, cost-effectiveness use disease-prone regions. conditional, self-limiting trait uses sex-determination gene doublesex linked tetracycline-off genetic switch cause complete lethality early larval development. With progeny survival, sex sorting longer required, eliminating large-scale production facilities or physical sex-separation. In deployment operations, this translates ability generate multiple generations each released, while being entirely self-limiting. To evaluate these potential benefits, trial was carried densely-populated urban, dengue-prone neighbourhoods Brazil, wherein able suppress wild populations by up 96%, demonstrating utility approach biological control. doing so, it shown such strains offer critical components necessary make tools highly accessible, thus they harbour transition sustainable within at-risk who may only limited resources.

Язык: Английский

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Weighing consequences of action and inaction in invasive insect management

One Earth, Год журнала: 2024, Номер 7(5), С. 782 - 793

Опубликована: Май 1, 2024

Язык: Английский

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Using problem formulation for an efficient, fit-for-purpose risk assessment of microbial plant protection products

Environmental Sciences Europe, Год журнала: 2025, Номер 37(1)

Опубликована: Фев. 8, 2025

Язык: Английский

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Gene drive in species complexes: defining target organisms

Trends in biotechnology, Год журнала: 2022, Номер 41(2), С. 154 - 164

Опубликована: Июль 19, 2022

Engineered gene drives share many environmental risk assessment considerations with other transgenes in genetically modified organisms, but they can differ significantly their potential to spread, increase frequency, and persist target populations.Recently, introduction of mosquitoes an engineered drive completely suppressed caged wild type laboratory populations the malaria vector Anopheles gambiae, belonging a species complex containing both nonvector that produce fertile interspecific hybrids.As sequences are conserved amongst all this complex, vertical transfer vectors nonvectors is plausible. This challenges notion simple dichotomy between organism nontarget organism.Using as specific case study, options on defining organisms complexes developed here, including proposal new concept complex. drives, which bias own inheritance frequency populations, being control mosquito vectors. Such belong hybrids, making (VGDT) sibling biologically While VGDT could positively impact human health protection goals, might challenge biodiversity ones. Therefore, use invites more nuanced than for not intended populations. Incorporating offers flexibility when assessing impacts from VGDT. Gene (see Glossary) allow genes, transgenes, or genetic traits be transmitted offspring at greater Mendelian frequencies, property modification interest spread through interbreeding [1.Burt A. Site-specific selfish genes tools engineering natural populations.Proc. Biol. Sci. 2003; 270: 921-928Crossref PubMed Scopus (442) Google Scholar, 2.Burt et al.Gene reduce transmission sub-Saharan Africa.J. Responsible Innov. 2018; 5: S66-S80Crossref (44) 3.Wang G.H. al.Symbionts drive: two strategies combat vector-borne disease.Trends Genet. 2022; 38: 708-723Abstract Full Text PDF (16) Scholar]. In particular, there considerable harnessing diseases such (Box 1) [4.African Union Development Agency – New Partnership Africa's Drives Malaria Control Elimination Africa.2018Google Scholar,5.World Health Organization Evaluation Genetically Modified Mosquitoes Vector-Borne Diseases. WHO, 2020Google Scholar].Box 1Malaria drivesThe World (WHO) has reported 2020 were 241 million cases worldwide, associated estimated 627 000 deaths [68.World Report. 2021Google Countries WHO African Region continue carry disproportionately high global burden, 95% 96% deaths. Plasmodium falciparum major pathogen responsible causing humans via bites infected female blood-feed hosts provide essential nutrients development eggs. As result insecticide-treated bed nets indoor insecticide spraying, well prophylactic therapeutic pharmaceutical treatments, been steady decline prevalence over last decade. However, progress recently stalled remains under further threat resistance behavioural adaptations, increased zoophilic responses, mosquitoes, drugs, invoking need complementary approaches burden disease, novel [3.Wang 4.African 5.World Scholar,68.World Scholar].Current include encoding CRISPR-Cas9 endonuclease expressed germline promoter, along ubiquitously constitutively gRNAs, together cleave genome [2.Burt Scholar,69.Hammond A.M. Galizi R. fight malaria: current state future directions.Pathog. Glob. Health. 111: 412-423Crossref (62) Once transgene introduced into its genomic location one pair homologous chromosomes, gRNA Cas9 act concert germ cells cause double-stranded break DNA site wild-type chromosome. When lesion repaired by homology directed repair using transgenic chromosome template, entire transgene, flanking either side pasted homologous, formerly wild-type, process homing create mostly pairs parental chromosomes homozygous so proportion would otherwise case, leading super-Mendelian progeny. Thus, once mating population, capable will drive, within assuming any fitness costs do outweigh The Current Before release considered regulators, decision-makers stakeholders, (ERA), whether probabilistic, qualitative, combination thereof, must conducted evaluate risks health, animal environment [6.National Academies Sciences, Engineering, Medicine Horizon: Advancing Science, Navigating Uncertainty, Aligning Research Public Values. National Press, 2016Google 7.Landis W.G. al.A general risk-based adaptive management scheme incorporating Bayesian Network Relative Risk Model South River, Virginia, study.Integr. Environ. Assess. Manag. 2017; 13: 115-126Crossref (26) 8.James S. al.Pathway deployment biocontrol tool elimination Sub-Saharan Africa: recommendations Scientific Working Group.Am. J. Trop. Med. Hyg. 98: 1-49Crossref (122) 9.EFSA Panel Organisms al.Adequacy sufficiency evaluation existing EFSA guidelines molecular characterisation, post-market monitoring insects drives.EFSA 2020; 18e06297Google 10.James S.L. al.Toward definition efficacy safety criteria advancing drive-modified field testing.Vector Borne Zoonotic Dis. 20: 237-251Crossref (38) 11.World Guidance Framework Testing Mosquitoes.2nd edn. 12.Ickowicz al.Predicting persistence dominant sterile male mosquitoes.Parasit. Vectors. 2021; 14: 480Crossref (4) 13.Connolly J.B. al.Recommendations applications control.Malar. 21: 152Crossref (9) A prerequisite effective ERA (GMOs) define unintended effects intervention (TOs) (NTOs) [9.EFSA Scholar,10.James Scholar,14.Romeis al.The value regulatory frameworks agricultural pest drives.Environ. Policy. 108: 19-36Crossref (23) 15.Devos Y. organisms: developing practical guidance.Trends Biotechnol. 39: 853-856Abstract (12) 16.Devos al.Potential against disease vectors, pests invasive poses assessment.Crit. Rev. : 1-17Google 17.Devos al.Risk releases insects.Biotechnol. Adv. 107807Crossref paradigm typically applied qualitative GM plants, where, general, clear distinction targeted, TOs, those NTOs. same GMOs, non-gene (GMMs), designed addition, most significant contain [18.Davidson G. species.Bull. Organ. 1964; 31: 625-634PubMed 19.Besansky N.J. al.Semipermeable boundaries gambiae arabiensis: evidence multilocus sequence variation.Proc. Natl. Acad. U. 100: 10818-10823Crossref (170) 20.Small S.T. al.Radiation reticulation marks origin vector.Proc. 117: 31583-31590Crossref (13) 21.Antonio-Nkondjio C. Simard F. Highlights anopheles nili moucheti, Africa.in: - insights IntechOpen, 2013Crossref 22.Crawford J.E. al.Reticulate speciation barriers introgression complex.Genome Evol. 2015; 7: 3116-3131Crossref (27) Scholar], some combinations hybridisation hybrids. semipermeable porous facilitate [19.Besansky Scholar,20.Small Scholar,23.Thelwell al.Evidence mitochondrial bwambae gambiae.Insect Mol. 2000; 9: 203-210Crossref (24) Scholar] plausibly lead species, nonvectors. represents binary choice TO NTO complexes. Depending how goals defined, evaluated number different ways ERA. Here, study involving population suppression sensu lato (s.l.) [24.Kyrou K. targeting doublesex causes complete mosquitoes.Nat. 36: 1062-1066Crossref (425) Scholar,25.Hammond al.Gene-drive large cages bridge lab field.Nat. Commun. 12: 4589Crossref (39) used illustrate these differing possibilities consequences 2).Box 2Case study: gambiaeThere principal control. replacement disrupt endogenous cargo prevent pathogens [69.Hammond density introducing cost sex bias. example, gRNAs haplosufficient fertility genes. Transgenic heterozygous females disrupted copy remain fertile. Via homing, rates males population. They therefore increasingly likely mate another, generating increasing proportions transgenics, sterile. progeny decreases suppressed.Early attempts suppress led caused mutations simultaneously retained functionality protein targeted [70.Hammond system reproduction gambiae.Nat. 2016; 34: 78-83Crossref (733) Recently, determination locus s.l. investigated drive. locus, highly conserved, encodes transcripts, (AgdsxM), (AgdsxF) spans intron–exon boundary, mutation selectively disrupts AgdsxF isoform, intersex, unable blood feed. laboratory, small eliminations after 9–11 generations recent cage experiments closely mimic feeding reproductive environments field, overlapping, age-structured collapse year without [25.Hammond There suppressed. Early Africa coluzzii, stricto (s.s.), arabiensis, funestus (all Diptera: Culicidae) [26.Sinka M.E. Africa, Europe Middle East: occurrence data, distribution maps bionomic precis.Parasit. 2010; 3: 117Crossref (503) Scholar,27.Afrane al.Secondary continent?.in: Rodriguez-Morales A.J. Topics Malaria. 2016Crossref s.s., arabiensis currently active [28.Burt Crisanti evolved synthetic.ACS Chem. 343-346Crossref (49) All three members 3). also contains species. Most have examined producing hybrids Some found albeit low frequencies [23.Thelwell Scholar,29.White G.B. Chromosomal hybridization complex.Nature. 1971; 231: 184-185Crossref (41) 30.Costantini al.Living edge: biogeographic patterns habitat segregation conform niche expansion gambiae.BMC Ecol. 2009; 16Crossref (156) 31.Pombi M. al.Dissecting functional components isolation among related sympatric complex.Evol. Appl. 10: 1102-1120Crossref (29) 32.Epopa P.S. al.Seasonal dynamics western Burkina Faso.Malar. 2019; 18: 113Crossref thus representing route.Box 3Anopheles complexThe recognised least nine morphologically indistinguishable shared common ancestor less 2 years ago [71.Fontaine M.C. al.Mosquito genomics. Extensive revealed phylogenomics.Science. 3471258524Crossref (337) Scholar]: amharicus, bwambae, fontenillei; melas, merus, quadriannulatus.A. coluzzii regional sections Scholar,72.Sinka map vectors.Parasit. 2012; 69Crossref (395) geographically restricted, local [48.White sp.n., Semliki Valley, Uganda, relationships An. (Diptera: Culicidae).Syst. Entomol. 1985; 501-522Crossref

Язык: Английский

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COMPARISON OF THE TERMS CREATIVE ACCOUNTING, EARNINGS MANAGEMENT AND FRAUDULENT ACCOUNTING THROUGH BIBLIOGRAPHIC ANALYSIS

Ekonomicko-manazerske spektrum, Год журнала: 2021, Номер 15(2), С. 27 - 37

Опубликована: Дек. 30, 2021

Research background: The article focuses on the issues of creative accounting, earnings management, and fraudulent which are global phenomena. These concepts well known globally, as they dealt with by many world-renowned authors. In this study, we applied bibliometric analysis to these reveal their interconnectedness. research was conducted a sample more than 19,000 articles. Purpose article: main goal study is use VosViewer design visualisation program capture record most common terms associated terms, ‘creative accounting’, ‘revenue management’, ‘fraudulent show biometric network commonly used terms. Methods: To illustrate important words above used, drew mind maps that represented expressions were closest topic. Scientific articles from Web Science database, contains world-class related topic, input data. Findings & Value added: results provided an interesting insight into keywords revenue accounting. phrases related, several them repeated in each mentioned. This means that, although different nature, nevertheless connected phrases. However, it remains necessary observe given appears colour fraud (white, grey, or black fraud).

Язык: Английский

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The Promise and Challenge of Genetic Biocontrol Approaches for Malaria Elimination

Tropical Medicine and Infectious Disease, Год журнала: 2023, Номер 8(4), С. 201 - 201

Опубликована: Март 29, 2023

Malaria remains an ongoing public health challenge, with over 600,000 deaths in 2021, of which approximately 96% occurred Africa. Despite concerted efforts, the goal global malaria elimination has stalled recent years. This resulted widespread calls for new control methods. Genetic biocontrol approaches, including those focused on gene-drive-modified mosquitoes (GDMMs), aim to prevent transmission by either reducing population size malaria-transmitting or making less competent transmit parasite. The development both strategies advanced considerably years, successful field trials several methods employing live mosquito products and demonstration efficacy GDMMs insectary-based studies. Live achieve area-wide characteristics that differ substantially from current insecticide-based vector methods, resulting some different considerations approval implementation. application technologies against other pests provides evidence promise these approaches insights into pathway agents. status technical as well thinking implementation requirements genetic are reviewed, remaining challenges prevention discussed.

Язык: Английский

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Leveraging eco-evolutionary models for gene drive risk assessment

Trends in Genetics, Год журнала: 2023, Номер 39(8), С. 609 - 623

Опубликована: Май 15, 2023

Язык: Английский

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Gene drives, mosquitoes, and ecosystems: an interdisciplinary approach to emerging ethical concerns

Frontiers in Environmental Science, Год журнала: 2024, Номер 11

Опубликована: Янв. 12, 2024

Gene drives are genetic elements that in sexually reproducing organisms spread faster than those transmitted through a Mendelian fashion. Since gene can be engineered to modify different aspects of physiology and reproduction, they have been proposed as new revolutionary tool control vector-borne diseases, particularly by the genera Anopheles Aedes (Culicidae), such malaria, Dengue Zika virus. This approach may impact on human health lowering transmission devastating diseases. However, release genetically modified mosquitos (or other species) into environment raises series questions related still incipient technology our present understanding complex structure dynamics terrestrial aquatic ecosystems. Moreover, there ethical concerns about interventions natural ecosystems eventually way living or themselves. work is an interdisciplinary analyzes from biological, philosophical, theological perspective potential ecological impacts environments species, focusing drive-modified mosquitos. It includes Catholic point view (although it could easily shared Christians) because we hold world religions give valuable insights even though not everyone share their groundings. We conclude focal problem relationship between humans nature, species change this unpredictably. given interactions ecosystems, approaches Earth Stewardship principles provide more widely accepted answers involving concepts will help engaging all relevant actors make better world.

Язык: Английский

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Genome editing in future crop protection: utilizing CRISPR/Cas9 to improve crop resistance against diseases, pests, and weeds

Discover Agriculture, Год журнала: 2024, Номер 2(1)

Опубликована: Ноя. 19, 2024

Abstract Increasing population and climate change pose significant threats to global food security by imposing stresses on plants, making them more susceptible diseases productivity losses caused pathogens, pests, weeds. Traditional breeding strategies are insufficient for rapid development of new plant traits that can outpace this downtrend. Modern advances in genome editing technologies, particularly CRISPR/Cas9, have revolutionised crop protection through precise targeted modifications. This allows the resilient crops with enhanced resistance against review explores various approaches which CRISPR/Cas9 is applied protection: knocking out susceptibility genes, introduction modulation defence genes. Potential applications include genes conferring disruption insect responsible survival reproduction engineering herbicide-resistant crops. In conclusion, holds great promises advancing thus ensuring amidst environmental pressures. highlights transformative potential calls continued research field.

Язык: Английский

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