Synthetic and Systems Biotechnology, Год журнала: 2025, Номер unknown
Опубликована: Май 1, 2025
Язык: Английский
Carbohydrate Polymers, Год журнала: 2023, Номер 324, С. 121524 - 121524
Опубликована: Окт. 31, 2023
Язык: Английский
Процитировано
28
Molecules, Год журнала: 2024, Номер 29(5), С. 1127 - 1127
Опубликована: Март 2, 2024
Microbial cell factories, renowned for their economic and environmental benefits, have emerged as a key trend in academic industrial areas, particularly the fermentation of natural compounds. Among these, plant-derived terpenes stand out significant class bioactive products. The large-scale production such terpenes, exemplified by artemisinic acid—a crucial precursor to artemisinin—is now feasible through microbial factories. In two-phase technology has been widely applied due its unique advantages. It facilitates situ product extraction or adsorption, effectively mitigating detrimental impact accumulation on cells, thereby significantly bolstering efficiency terpenes. This paper reviews latest developments system applications, focusing also discusses mechanisms influencing biosynthesis Moreover, we introduce some new techniques, currently unexplored terpene fermentation, with aim providing more thoughts explorations future applications technology. Lastly, discuss several challenges application systems, especially downstream processing.
Язык: Английский
Процитировано
12
Foods, Год журнала: 2025, Номер 14(4), С. 673 - 673
Опубликована: Фев. 17, 2025
Microbial foods include microbial biomass, naturally fermented foods, and heterologously synthesized food ingredients derived from fermentation. Terpenoids, using isoprene as the basic structure, possess various skeletons functional groups. They exhibit diverse physicochemical properties physiological activities, such unique flavor, anti-bacterial, anti-oxidant, anti-cancer, hypolipemic, making them extensively used in industry, fragrance, preservatives, dietary supplements, medicinal health food. Compared to traditional strategies like direct extraction natural species chemical synthesis, cell factories for edible terpenoids have higher titers yields. can utilize low-cost raw materials are easily scaling-up, representing a novel green sustainable production mode. In this review, we briefly introduce synthetic pathway of applications producing terpenoids. Secondly, highlight several typical non-typical chassis terpenoid-producing factories. addition, reviewed recent advances representative terpenoid with gram-scale titer preservation, nutritional enhancers, foods. Finally, predict future directions their commercialization process.
Язык: Английский
Процитировано
2
Chemistry - A European Journal, Год журнала: 2024, Номер 30(19)
Опубликована: Фев. 15, 2024
In this review, the impact of transition from today's resource-wasting petrochemical economy towards a 100/100 renewable and biodegradable future is discussed with respect to fragrance families: "citrus", "green", "fruity", "floral", "floriental", "oriental", "woody", "chypre" "fougère". After benchmark data on ingredients usage, definitions biodegradation sustainability are given. Celebrating 150
Язык: Английский
Процитировано
8
Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(33), С. 18305 - 18320
Опубликована: Июль 5, 2024
Flavors and fragrances (F&F) are interesting organic compounds in chemistry. These widely used the food, cosmetic, medical industries. Enzymatic synthesis exhibits several advantages over natural extraction chemical preparation, including a high yield, stable quality, mildness, environmental friendliness. To date, many oxidoreductases hydrolases have been to biosynthesize F&F. Ene-reductases (ERs) class of biocatalysts that can catalyze asymmetric reduction α,β-unsaturated offer superior specificity selectivity; therefore, ERs increasingly considered an ideal alternative their counterparts. This review summarizes research progress on use F&F past 20 years, achievements various scholars, differences similarities among findings, discussions future trends related ERs. We hope this inspire researchers promote development biotechnology industry.
Язык: Английский
Процитировано
7
Current Opinion in Food Science, Год журнала: 2024, Номер 61, С. 101242 - 101242
Опубликована: Ноя. 12, 2024
Язык: Английский
Процитировано
7
Synthetic and Systems Biotechnology, Год журнала: 2025, Номер 10(2), С. 433 - 441
Опубликована: Янв. 22, 2025
Язык: Английский
Процитировано
1
Microorganisms, Год журнала: 2024, Номер 12(4), С. 706 - 706
Опубликована: Март 30, 2024
High-energy-density liquid fuels (HED fuels) are essential for volume-limited aerospace vehicles and could serve as energetic additives conventional fuels. Terpene-derived HED biofuel is an important research field green fuel synthesis. The direct extraction of terpenes from natural plants environmentally unfriendly costly. Designing efficient synthetic pathways in microorganisms to achieve high yields shows great potential the application terpene-derived This review provides overview current progress fuels, surveying terpene properties status biosynthesis. Additionally, we systematically summarize engineering strategies biosynthesizing terpenes, including mining synthases, optimizing metabolic cell-level optimization, such subcellular localization synthesis adaptive evolution. article will be helpful providing insight into better developing
Язык: Английский
Процитировано
4
International Journal of Biological Macromolecules, Год журнала: 2024, Номер 279, С. 135377 - 135377
Опубликована: Сен. 6, 2024
Язык: Английский
Процитировано
4
Frontiers in Microbiology, Год журнала: 2025, Номер 16
Опубликована: Март 31, 2025
The sustainable production of valuable compounds using microbial cell factories is an effective approach, yet further metabolic engineering strategies are needed to enhance their biosynthetic potential. Recent studies suggest that RNA modifications can influence cellular metabolism, but role in remains largely unexplored. squalene and lupeol different yeast strains was detected by gas chromatography-mass spectrometry (GC-MS) equipment. Transcriptomic analysis performed identify changes associated with the epigenetic modification. transcriptional translational expression targeted genes were determined real-time quantitative polymerase chain reaction western blotting, respectively. mRNA stability measured decay assay. In this study, overexpression Trm8 Trm82 complex, mediating tRNA 7-methylguanosine (m7G) modification yeast, significantly increased CEN.PK2-1C. Transcriptome indicated Trm8/Trm82 upregulated levels involved amino acid synthesis, glycolysis, tricarboxylic cycle, enhanced upstream acetyl-CoA biosynthesis, might be responsible for promoted biosynthesis squalene. Further investigation demonstrated complex could increase engineered yeast. These findings indicate m7G regulate central metabolism terpenoid biosynthesis. This study provides new insights into as a potential strategy improving high-value compounds.
Язык: Английский
Процитировано
0