3D-Printed Polycaprolactone/Hydroxyapatite Bionic Scaffold for Bone Regeneration
Polymers,
Год журнала:
2025,
Номер
17(7), С. 858 - 858
Опубликована: Март 23, 2025
The
limitations
of
traditional,
autologous
bone
grafts,
such
as
the
scarcity
donor
material
and
risks
secondary
surgical
trauma,
have
spurred
development
alternatives
for
repair
large
defects.
Bionic
scaffolds
fabricated
via
fused
deposition
modeling
(FDM)-a
three-dimensional
(3D)
printing
technique-are
considered
promising.
While
gyroid-structured
mimic
complex
micro-architecture
cancellous
bone,
their
application
in
FDM
3D
remains
understudied.
Furthermore,
no
consensus
has
been
reached
on
ideal
pore
size
gyroid
scaffolds,
which
is
influenced
by
infill
density.
In
this
study,
we
five
groups
polycaprolactone/hydroxyapatite
(PCL/HA)
with
different
densities
(40%,
45%,
50%,
55%,
60%)
using
a
solvent-free
filament
preparation
method.
Scanning
electron
microscopy
(SEM)
observation
showed
that
all
exhibit
an
interconnected
porous
structure.
scaffold
55%
density,
featuring
465
±
63
μm,
demonstrated
optimal
hydrophilicity
mechanical
properties
comparable
to
natural
bone.
addition,
supported
cellular
bridging
within
its
pores
highest
alkaline
phosphatase
(ALP)
activity
calcium
salt
deposition.
Our
findings
offer
novel
insights
into
design
gyroid-like
fabrication
FDM,
paving
way
potential
clinical
applications.
Язык: Английский
Knockdown of IER3 Promotes Osteogenic Differentiation of Human Mesenchymal Stem Cells
Biomedicines,
Год журнала:
2025,
Номер
13(4), С. 947 - 947
Опубликована: Апрель 12, 2025
Background:
The
differentiation
process
of
human
mesenchymal
stem
cells
(hMSCs)
is
regulated
by
a
variety
chemical,
physical,
and
biological
factors.
These
factors
activate
distinct
signaling
pathways
transcriptional
networks,
thereby
regulating
the
lineage-specific
hMSCs.
Objective:
This
study
aims
to
investigate
role
Immediate
Early
Response
3
(IER3)
in
osteogenic
explore
underlying
regulatory
mechanisms
which
IER3
influences
osteogenesis.
Methods:
expression
levels
osteogenesis-related
genes
were
quantified
when
hMSCs
subjected
vitro
induction.
Then,
stable
IER3–knockdown
generated
using
IER3–targeted
shRNA
lentiviral
vectors,
impact
on
was
evaluated
through
both
cell
induction
subcutaneous
implantation
model
nude
mice.
Moreover,
RNA–seq
functional
inhibition
assays
performed
elucidate
pathway
regulates
Results:
significantly
downregulated
during
differentiation.
Knockdown
markedly
upregulated
ALP
RUNX2,
enhancing
capacity
hMSCs,
vivo.
Mechanistic
studies
revealed
that
knockdown
increased
phosphorylated
ERK1/2
levels,
activating
MAPK/ERK
pathway.
Furthermore,
reversed
enhanced
observed
following
knockdown.
Conclusions:
promotes
regulation
pathway,
indicating
represents
potential
therapeutic
target
for
treatment
osteoporosis
bone
defect-related
diseases.
Язык: Английский
Research Progress on the Role and Intervention of the MAPK Signaling Pathway in the Imbalance of Bone Remodeling in Postmenopausal Osteoporosis
Advances in Clinical Medicine,
Год журнала:
2025,
Номер
15(04), С. 2483 - 2493
Опубликована: Янв. 1, 2025
Язык: Английский
Low Expression of Selenoprotein S Promotes Osteogenic Differentiation in Bone Marrow Mesenchymal Stromal Cells
Biological Trace Element Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 8, 2025
Язык: Английский
CREG1 alleviates bone loss in osteoporosis by enhancing the osteogenic differentiation of BMSCs through mitophagy
International Immunopharmacology,
Год журнала:
2025,
Номер
158, С. 114867 - 114867
Опубликована: Май 15, 2025
Язык: Английский
NAD+/Nrf2 signaling promotes osteogenesis by regulating oxidative level of BMSCs under mechanical stress
Progress in Orthodontics,
Год журнала:
2025,
Номер
26(1)
Опубликована: Май 30, 2025
Abstract
Background
Mechanical
stress
triggers
an
increase
in
cellular
reactive
oxygen
species
(ROS),
which
is
associated
with
the
impairment
of
osteogenesis.
During
orthodontic
treatment,
bone
marrow
mesenchymal
stem
cells
(BMSCs)
experience
mechanical
stress,
yet
oxidative
profile
and
redox
regulatory
mechanisms
under
such
especially
involving
Nicotinamide
adenine
dinucleotide
(NAD
+
),
are
not
well
understood,
necessitating
further
research
into
their
roles
therapies.
Methods
The
Tension
System
was
established
to
detect
ROS
changes
BMSCs
cyclic
stretch
H
2
O
simulating
uncontrolled
ROS.
Flow
cytometry
fluorescence
staining
measured
ROS,
while
NAD
/NADH
assay
kit
assessed
levels.
qRT-PCR
Western
blotting
analyzed
expression
synthesis
consume
enzymes.
Osteogenic
potential
evaluated
by
qRT-PCR,
blotting,
Alkaline
phosphatase
(ALP)
staining.
Loss-of-function
supplementation
assays
explored
role
Nrf2
regulation,
localization
immunofluorescence
blotting.
In
vivo
osteogenic
effects
were
confirmed
using
tooth
movement
(OTM)
model,
osteogenesis
immunohistochemistry
microCT
for
OTM
measurements.
Results
Cyclic
increased
over
24
h
boosted
differentiation.
However,
from
hindered
this
process.
Notably,
levels
rose
stretch,
experiments
showed
it
supported
controlling
level
BMSCs.
Furthermore,
regulated
BMSC
via
nuclear
translocation.
Rat
models
indicated
that
NMN
enhanced
osteoclastic
markers
accelerated
movement,
FK866
inhibited
effect.
Conclusions
We
identified
/Nrf2
signaling
thus
promoted
commitment
stress.
Targeting
metabolism
or
administrating
exogenous
promote
rebuilding
could
be
a
prospective
therapy
accelerate
OTM.
Язык: Английский
Effects and mechanisms of berberine-loaded tetrahedral framework nucleic acids on osteoporosis
Journal of Materials Chemistry B,
Год журнала:
2024,
Номер
12(41), С. 10705 - 10718
Опубликована: Янв. 1, 2024
Osteoporosis
is
a
disease
that
plagues
the
world,
complications
of
which,
spinal
fractures
and
hip
fractures,
seriously
endanger
health
elderly.
At
present,
main
treatment
for
osteoporosis
anti-osteoporosis
drugs,
some
however,
have
poor
efficacy
side
effects.
Thus,
development
new
drugs
clinical
significance.
Berberine
(BBR)
has
good
therapeutic
effect
on
osteoporosis,
but
its
solubility
instability
limit
effect.
Tetrahedral
framework
nucleic
acids
(tFNAs)
are
novel
nano-drug
delivery
tool,
their
cell
entry
ability
stability
make
them
an
excellent
carrier.
Therefore,
nanocomplex
T-BBR
was
synthesized
by
carrying
BBR
with
tFNAs.
Compared
BBR,
showed
better
osteogenic
abilities
in
both
Язык: Английский