Effects and mechanisms of berberine-loaded tetrahedral framework nucleic acids on osteoporosis DOI
Weifei Zhang, Chuan Fei Guo, Dengbo Yao

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(41), P. 10705 - 10718

Published: Jan. 1, 2024

Osteoporosis is a disease that plagues the world, complications of which, spinal fractures and hip fractures, seriously endanger health elderly. At present, main treatment for osteoporosis anti-osteoporosis drugs, some however, have poor efficacy side effects. Thus, development new drugs clinical significance. Berberine (BBR) has good therapeutic effect on osteoporosis, but its solubility instability limit effect. Tetrahedral framework nucleic acids (tFNAs) are novel nano-drug delivery tool, their cell entry ability stability make them an excellent carrier. Therefore, nanocomplex T-BBR was synthesized by carrying BBR with tFNAs. Compared BBR, showed better osteogenic abilities in both

Language: Английский

3D-Printed Polycaprolactone/Hydroxyapatite Bionic Scaffold for Bone Regeneration DOI Open Access
Fengze Wang, Shuo Liu, Min Gao

et al.

Polymers, Journal Year: 2025, Volume and Issue: 17(7), P. 858 - 858

Published: March 23, 2025

The limitations of traditional, autologous bone grafts, such as the scarcity donor material and risks secondary surgical trauma, have spurred development alternatives for repair large defects. Bionic scaffolds fabricated via fused deposition modeling (FDM)-a three-dimensional (3D) printing technique-are considered promising. While gyroid-structured mimic complex micro-architecture cancellous bone, their application in FDM 3D remains understudied. Furthermore, no consensus has been reached on ideal pore size gyroid scaffolds, which is influenced by infill density. In this study, we five groups polycaprolactone/hydroxyapatite (PCL/HA) with different densities (40%, 45%, 50%, 55%, 60%) using a solvent-free filament preparation method. Scanning electron microscopy (SEM) observation showed that all exhibit an interconnected porous structure. scaffold 55% density, featuring 465 ± 63 μm, demonstrated optimal hydrophilicity mechanical properties comparable to natural bone. addition, supported cellular bridging within its pores highest alkaline phosphatase (ALP) activity calcium salt deposition. Our findings offer novel insights into design gyroid-like fabrication FDM, paving way potential clinical applications.

Language: Английский

Citations

0
Research Progress on the Role and Intervention of the MAPK Signaling Pathway in the Imbalance of Bone Remodeling in Postmenopausal Osteoporosis DOI

雍霖 颜

Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(04), P. 2483 - 2493

Published: Jan. 1, 2025

Language: Английский

Citations

0
Knockdown of IER3 Promotes Osteogenic Differentiation of Human Mesenchymal Stem Cells DOI Creative Commons
Yang Han, Hongyang Ma, Zhihui Tang

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 947 - 947

Published: April 12, 2025

Background: The differentiation process of human mesenchymal stem cells (hMSCs) is regulated by a variety chemical, physical, and biological factors. These factors activate distinct signaling pathways transcriptional networks, thereby regulating the lineage-specific hMSCs. Objective: This study aims to investigate role Immediate Early Response 3 (IER3) in osteogenic explore underlying regulatory mechanisms which IER3 influences osteogenesis. Methods: expression levels osteogenesis-related genes were quantified when hMSCs subjected vitro induction. Then, stable IER3–knockdown generated using IER3–targeted shRNA lentiviral vectors, impact on was evaluated through both cell induction subcutaneous implantation model nude mice. Moreover, RNA–seq functional inhibition assays performed elucidate pathway regulates Results: significantly downregulated during differentiation. Knockdown markedly upregulated ALP RUNX2, enhancing capacity hMSCs, vivo. Mechanistic studies revealed that knockdown increased phosphorylated ERK1/2 levels, activating MAPK/ERK pathway. Furthermore, reversed enhanced observed following knockdown. Conclusions: promotes regulation pathway, indicating represents potential therapeutic target for treatment osteoporosis bone defect-related diseases.

Language: Английский

Citations

0
Low Expression of Selenoprotein S Promotes Osteogenic Differentiation in Bone Marrow Mesenchymal Stromal Cells DOI
Hao Wu, Jingyi Shi,

Yunshan Zhao

et al.

Biological Trace Element Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Language: Английский

Citations

0
Effects and mechanisms of berberine-loaded tetrahedral framework nucleic acids on osteoporosis DOI
Weifei Zhang, Chuan Fei Guo, Dengbo Yao

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(41), P. 10705 - 10718

Published: Jan. 1, 2024

Osteoporosis is a disease that plagues the world, complications of which, spinal fractures and hip fractures, seriously endanger health elderly. At present, main treatment for osteoporosis anti-osteoporosis drugs, some however, have poor efficacy side effects. Thus, development new drugs clinical significance. Berberine (BBR) has good therapeutic effect on osteoporosis, but its solubility instability limit effect. Tetrahedral framework nucleic acids (tFNAs) are novel nano-drug delivery tool, their cell entry ability stability make them an excellent carrier. Therefore, nanocomplex T-BBR was synthesized by carrying BBR with tFNAs. Compared BBR, showed better osteogenic abilities in both

Language: Английский

Citations

0