Construction and Osteogenic Capacity Study of Engineered Exosome-based Nanoplatform Targeting Macrophages

Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер unknown, С. 106912 - 106912

Опубликована: Апрель 1, 2025

Язык: Английский

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Apigenin inhibits tumor angiogenesis by hindering microvesicle biogenesis via ARHGEF1

Cancer Letters, Год журнала: 2024, Номер 596, С. 216961 - 216961

Опубликована: Май 31, 2024

Язык: Английский

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Lysosomal exocytosis: From cell protection to protumoral functions

Cancer Letters, Год журнала: 2024, Номер 597, С. 217024 - 217024

Опубликована: Июнь 12, 2024

Lysosomes are single membrane bounded group of acidic organelles that can be involved in a process called lysosomal exocytosis which leads to the extracellular release their content. Lysosomal is required for plasma repair or remodeling events such as bone resorption, antigen presentation mitosis, and protection against toxic agents heavy metals. Recently, it has been showed fulfill this protective role, needs some autophagic proteins, an autophagy-independent manner. In addition these crucial physiological roles, plays major protumoral role various cancers. This effect exerted through tumor microenvironment modifications, including matrix remodeling, acidosis, oncogenic profibrogenic signals. review provides comprehensive overview different elements released during exocytosis, i.e. proteases, exosomes, protons, effects context development treatment.

Язык: Английский

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Transcriptional switches in melanoma T Cells: Facilitating polarizing into regulatory T cells

International Immunopharmacology, Год журнала: 2024, Номер 137, С. 112484 - 112484

Опубликована: Июнь 16, 2024

Melanoma is a malignant skin tumor with high mortality rate. Regulatory T cells (Tregs) are immune immunosuppressive roles, however, the precise mechanisms governing Treg involvement in melanoma remain enigmatic. Experimental findings unveiled different transcription factor switches between normal and cell, heightened FOXP3 BATF latter. These factors induced molecules maintenance genes, polarizing into Tregs. Spatial transcriptomics illuminated preferential settlement of Tregs at periphery. Within this context, facilitated direct enhancement specific ligand gene expression, fostering communication neighboring cells. Novel functional bound to or Tregs, such as SPOCK2, SH2D2A, ITGB2, LTA, CLEC2C, CLEC2D, were discovered, which had not been previously reported studies. Furthermore, we validated our large number clinical samples identified Treg-Specific Tag Set (Mel TregS). ELISA analysis showed that protein levels Mel TregS higher than We then utilized SERS technology measure signal values exosome, successfully discriminated healthy individuals patients, well early late-stage patients. This approach significantly enhanced detection sensitivity. In sum, research elucidated fresh insights self-maintenance surrounding melanoma. also introduced an innovative method for disease monitoring through technology.

Язык: Английский

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Review of pre-metastatic niches induced by osteosarcoma-derived extracellular vesicles in lung metastasis: A potential opportunity for diagnosis and intervention

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 178, С. 117203 - 117203

Опубликована: Июль 26, 2024

Osteosarcoma (OS) has a high propensity for lung metastasis, which is the leading cause of OS-related death and treatment failure. Intercellular communication between OS cells distant host required successful metastasis to lung. Before infiltrate lung, in situ secrete extracellular vesicles (EVs) that act as mediators cell-to-cell communication. In recent years, EVs have been confirmed bridges key drivers tumors metastatic lesions by regulating formation pre-metastatic niche (PMN), defined microenvironment suitable disseminated tumor cell engraftment colonization, target organs. This review summarizes current knowledge about underlying mechanisms PMN induced OS-derived potential roles targets or drug carriers We also provide an overview their EV-based therapeutic strategies hindering context metastasis.

Язык: Английский

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Unveiling the Dynamic Interplay between Cancer Stem Cells and the Tumor Microenvironment in Melanoma: Implications for Novel Therapeutic Strategies

Cancers, Год журнала: 2024, Номер 16(16), С. 2861 - 2861

Опубликована: Авг. 16, 2024

Cutaneous melanoma still represents a significant health burden worldwide, being responsible for the majority of skin cancer deaths. Key advances in therapeutic strategies have significantly improved patient outcomes; however, most patients experience drug resistance and tumor relapse. Cancer stem cells (CSCs) are small subpopulation different tumors, including melanoma, endowed with distinctive capacities self-renewal differentiation into bulk cells. Melanoma CSCs characterized by expression specific biomarkers intracellular pathways; moreover, they play pivotal role onset, progression resistance. In recent years, great efforts been made to dissect molecular mechanisms underlying protumor activities provide basis novel CSC-targeted therapies. Herein, we highlight intricate crosstalk between bystander microenvironment (TME), immune cells, endothelial cancer-associated fibroblasts (CAFs), its progression. Specifically, discuss peculiar escape host surveillance, recruit immunosuppressive educate toward an phenotype. We also address currently investigated that could pave way new promising approaches care.

Язык: Английский

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Extracellular vesicle analysis in supramolecular 3D hydrogels: a proof-of-concept

Sensors & Diagnostics, Год журнала: 2024, Номер 3(3), С. 395 - 399

Опубликована: Янв. 1, 2024

Here we report a proof-of-concept application of composite Aga-Q3 hydrogel for the gentle confinement and analysis extracellular vesicles (EVs) on microarray analytical platforms.

Язык: Английский

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Aptamer-based Membrane Protein Analysis and Molecular Diagnostics

Chemical Research in Chinese Universities, Год журнала: 2024, Номер 40(2), С. 173 - 189

Опубликована: Март 12, 2024

Язык: Английский

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Emerging role and translational potential of small extracellular vesicles in neuroscience

Life Sciences, Год журнала: 2024, Номер 355, С. 122987 - 122987

Опубликована: Авг. 14, 2024

Язык: Английский

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The sEVs miR-487a/Notch2/GATA3 axis promotes osteosarcoma lung metastasis by inducing macrophage polarization toward the M2-subtype

Cancer Cell International, Год журнала: 2024, Номер 24(1)

Опубликована: Авг. 31, 2024

Small extracellular vesicles (sEVs) are important mediators of intercellular communication between tumor cells and their surrounding environment. Furthermore, the mechanisms by which miRNAs carried in sEVs regulate macrophage polarization remain largely unknown. To concentrate sEVs, we used traditional ultracentrifugation method. Western blot, NanoSight, transmission electron microscopy were to identify sEVs. determine function sEVs-miR-487a, conducted vivo vitro investigations. The mechanism osteosarcoma M2 macrophages, mediated carrying miR-487a, was validated using luciferase reporter assays, transwell blot analysis. In vitro, enriched miR-487a delivered promoting toward an M2-like type, promotes proliferation, migration, invasion, epithelial-mesenchymal transition (EMT) cells. vivo, facilitate lung metastasis osteosarcoma. Moreover, plasma shown be a potential biomarker applicable for diagnosis. summary, derived from can transferred macrophages via then promote towards type targeting Notch2 activating GATA3 pathway. feedback loop, activation accelerates (EMT), turn This reciprocal interaction activated contributes progression disease. Our data demonstrate new that exosomal-miR-487a is involved development regulating microenvironment (TME).

Язык: Английский

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