METTL14-mediated m6A modification upregulates HOXB13 expression to activate NF-κB and exacerbate cervical cancer progression DOI Creative Commons
Qian Li, Na Zhao, Xiao Ding

и другие.

Molecular & Cellular Oncology, Год журнала: 2024, Номер 11(1)

Опубликована: Ноя. 11, 2024

Cervical cancer (CC) is one of the common malignant tumors in women, and incidence rate located second place female tumors. As a major RNA N6-methyladenosine (m6A) methyltransferase, methyltransferase-like 14 (METTL14) involved tumor progression by catalyzing methylation modifications mRNAs. However, molecular mechanism METTL14-mediated m6A modification CC remains not fully revealed. The expression METTL14 was detected RT-qPCR western blot. Cell function assayed cell counting kit-8 (CCK-8) assay flow cytometry analysis. Methylated immunoprecipitation (MeRIP) used to confirm relationship between homeobox B13 (HOXB13). In our study, we found that level elevated tissues cells compared with their controls. inhibition significantly impaired proliferation epithelial-mesenchymal transition (EMT) process, while also induced apoptosis HeLa C33A cells. Furthermore, findings indicated (HOXB13) target METTL14, which positively regulated HOXB13 an m6A-dependent manner. Rescue experiments overexpression effectively reversed suppression knockdown. Finally, confirmed METTL14-modified exerted oncogenic effect through activation nuclear factor kappa B (NF-κB) pathway. conclusion, data demonstrated HOXB13, mediated facilitated advancement targeting NF-κB pathway, may be potential for treatment CC.

Язык: Английский

CircZMYM2 plays a pivotal role in osteosarcoma by regulating the translation of NACA and ARPC1B DOI Creative Commons

Fang Li,

Jiacheng Wei, Xiaolu Ren

и другие.

Heliyon, Год журнала: 2024, Номер 10(22), С. e37744 - e37744

Опубликована: Сен. 10, 2024

Язык: Английский

Процитировано

0
METTL14-mediated m6A modification upregulates HOXB13 expression to activate NF-κB and exacerbate cervical cancer progression DOI Creative Commons
Qian Li, Na Zhao, Xiao Ding

и другие.

Molecular & Cellular Oncology, Год журнала: 2024, Номер 11(1)

Опубликована: Ноя. 11, 2024

Cervical cancer (CC) is one of the common malignant tumors in women, and incidence rate located second place female tumors. As a major RNA N6-methyladenosine (m6A) methyltransferase, methyltransferase-like 14 (METTL14) involved tumor progression by catalyzing methylation modifications mRNAs. However, molecular mechanism METTL14-mediated m6A modification CC remains not fully revealed. The expression METTL14 was detected RT-qPCR western blot. Cell function assayed cell counting kit-8 (CCK-8) assay flow cytometry analysis. Methylated immunoprecipitation (MeRIP) used to confirm relationship between homeobox B13 (HOXB13). In our study, we found that level elevated tissues cells compared with their controls. inhibition significantly impaired proliferation epithelial-mesenchymal transition (EMT) process, while also induced apoptosis HeLa C33A cells. Furthermore, findings indicated (HOXB13) target METTL14, which positively regulated HOXB13 an m6A-dependent manner. Rescue experiments overexpression effectively reversed suppression knockdown. Finally, confirmed METTL14-modified exerted oncogenic effect through activation nuclear factor kappa B (NF-κB) pathway. conclusion, data demonstrated HOXB13, mediated facilitated advancement targeting NF-κB pathway, may be potential for treatment CC.

Язык: Английский

Процитировано

0