METTL14-mediated m6A modification upregulates HOXB13 expression to activate NF-κB and exacerbate cervical cancer progression DOI Creative Commons
Qian Li, Na Zhao, Xiao Ding

et al.

Molecular & Cellular Oncology, Journal Year: 2024, Volume and Issue: 11(1)

Published: Nov. 11, 2024

Cervical cancer (CC) is one of the common malignant tumors in women, and incidence rate located second place female tumors. As a major RNA N6-methyladenosine (m6A) methyltransferase, methyltransferase-like 14 (METTL14) involved tumor progression by catalyzing methylation modifications mRNAs. However, molecular mechanism METTL14-mediated m6A modification CC remains not fully revealed. The expression METTL14 was detected RT-qPCR western blot. Cell function assayed cell counting kit-8 (CCK-8) assay flow cytometry analysis. Methylated immunoprecipitation (MeRIP) used to confirm relationship between homeobox B13 (HOXB13). In our study, we found that level elevated tissues cells compared with their controls. inhibition significantly impaired proliferation epithelial-mesenchymal transition (EMT) process, while also induced apoptosis HeLa C33A cells. Furthermore, findings indicated (HOXB13) target METTL14, which positively regulated HOXB13 an m6A-dependent manner. Rescue experiments overexpression effectively reversed suppression knockdown. Finally, confirmed METTL14-modified exerted oncogenic effect through activation nuclear factor kappa B (NF-κB) pathway. conclusion, data demonstrated HOXB13, mediated facilitated advancement targeting NF-κB pathway, may be potential for treatment CC.

Language: Английский

CircZMYM2 plays a pivotal role in osteosarcoma by regulating the translation of NACA and ARPC1B DOI Creative Commons

Fang Li,

Jiacheng Wei, Xiaolu Ren

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(22), P. e37744 - e37744

Published: Sept. 10, 2024

Language: Английский

Citations

0
METTL14-mediated m6A modification upregulates HOXB13 expression to activate NF-κB and exacerbate cervical cancer progression DOI Creative Commons
Qian Li, Na Zhao, Xiao Ding

et al.

Molecular & Cellular Oncology, Journal Year: 2024, Volume and Issue: 11(1)

Published: Nov. 11, 2024

Cervical cancer (CC) is one of the common malignant tumors in women, and incidence rate located second place female tumors. As a major RNA N6-methyladenosine (m6A) methyltransferase, methyltransferase-like 14 (METTL14) involved tumor progression by catalyzing methylation modifications mRNAs. However, molecular mechanism METTL14-mediated m6A modification CC remains not fully revealed. The expression METTL14 was detected RT-qPCR western blot. Cell function assayed cell counting kit-8 (CCK-8) assay flow cytometry analysis. Methylated immunoprecipitation (MeRIP) used to confirm relationship between homeobox B13 (HOXB13). In our study, we found that level elevated tissues cells compared with their controls. inhibition significantly impaired proliferation epithelial-mesenchymal transition (EMT) process, while also induced apoptosis HeLa C33A cells. Furthermore, findings indicated (HOXB13) target METTL14, which positively regulated HOXB13 an m6A-dependent manner. Rescue experiments overexpression effectively reversed suppression knockdown. Finally, confirmed METTL14-modified exerted oncogenic effect through activation nuclear factor kappa B (NF-κB) pathway. conclusion, data demonstrated HOXB13, mediated facilitated advancement targeting NF-κB pathway, may be potential for treatment CC.

Language: Английский

Citations

0