Let’s make it personal: CRISPR tools in manipulating cell death pathways for cancer treatment
Cell Biology and Toxicology,
Год журнала:
2024,
Номер
40(1)
Опубликована: Июль 29, 2024
Abstract
Advancements
in
the
CRISPR
technology,
a
game-changer
experimental
research,
have
revolutionized
various
fields
of
life
sciences
and
more
profoundly,
cancer
research.
Cell
death
pathways
are
among
most
deregulated
cells
considered
as
critical
aspects
development.
Through
decades,
our
knowledge
mechanisms
orchestrating
programmed
cellular
has
increased
substantially,
attributed
to
revolution
cutting-edge
technologies.
The
heroic
appearance
systems
expanded
available
screening
platform
genome
engineering
toolbox
detect
mutations
create
precise
edits.
In
that
context,
ability
this
system
for
identification
targeting
cell
signaling
result
development
therapy
resistance
is
an
auspicious
choice
transform
accelerate
individualized
therapy.
concept
personalized
stands
on
molecular
characterization
individual
tumor
its
microenvironment
order
provide
treatment
with
highest
possible
outcome
minimum
toxicity.
This
study
explored
potential
technology
precision
by
identifying
specific
pathways.
It
showed
promise
finding
key
components
involved
death,
making
it
tool
targeted
However,
also
highlighted
challenges
limitations
need
be
addressed
future
research
fully
realize
treatment.
Graphical
abstract
Current
application
through
glance.
A
choosing
appropriate
biological
model
vitro
(using
established
lines,
animal
derived
cells,
human
stem
or
T
cells),
vivo
models
which
can
harbor
tumor),
ex
(human/animal-derived
organoids).
B
preparation
gRNA
library.
C
design
screening,
desired
gRNAs
phenotypic
response.
D
CRISPR-Cas
identified
targets,
Cas9
gene
editing
(Knockout,
base
editing,
prime
editing),
RNA
modulation
(modulation
splicing,
interference),
epigenomic
edits
interference/activation
using
dead
(dCas9)
(Bock
et
al.
2022b)
Язык: Английский
Ferroptosis and myocardial ischemia-reperfusion: mechanistic insights and new therapeutic perspectives
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Окт. 1, 2024
Myocardial
ischemia-reperfusion
injury
(MIRI)
is
a
significant
factor
in
the
development
of
cardiac
dysfunction
following
myocardial
infarction.
Ferroptosis,
type
regulated
cell
death
driven
by
iron
and
marked
lipid
peroxidation,
has
garnered
growing
interest
for
its
crucial
involvement
pathogenesis
MIRI.This
review
comprehensively
examines
mechanisms
ferroptosis,
focusing
on
regulation
through
metabolism,
VDAC
signaling,
antioxidant
system
dysregulation.
We
also
compare
ferroptosis
with
other
forms
to
highlight
distinct
characteristics.
Furthermore,
MIRI
examined
focus
recent
discoveries
concerning
ROS
generation,
mitochondrial
impairment,
autophagic
processes,
ER
stress,
non-coding
RNA
regulation.
Lastly,
emerging
therapeutic
strategies
that
inhibit
mitigate
are
reviewed,
providing
new
insights
into
potential
clinical
applications.
Язык: Английский
Targeting Ferroptosis in Rare Neurological Disorders Including Pediatric Conditions: Innovations and Therapeutic Challenges
Biomedicines,
Год журнала:
2025,
Номер
13(2), С. 265 - 265
Опубликована: Янв. 22, 2025
Ferroptosis,
characterized
by
iron
dependency
and
lipid
peroxidation,
has
emerged
as
a
key
mechanism
underlying
neurodegeneration
in
rare
neurological
disorders.
These
conditions,
often
marked
significant
therapeutic
gaps
high
unmet
medical
needs,
present
unique
challenges
for
intervention
development.
This
review
examines
the
involvement
of
ferroptosis
disease
pathogenesis,
focusing
on
its
role
oxidative
damage
neuronal
dysfunction.
We
explore
recent
pharmacological
advancements,
including
chelators,
peroxidation
blockers,
antioxidant-based
strategies,
designed
to
target
ferroptosis.
While
these
approaches
show
promise,
such
heterogeneity,
limited
diagnostic
tools,
small
patient
cohorts
hinder
progress.
Furthermore,
we
discuss
translational
regulatory
barriers
implementing
ferroptosis-based
therapies
clinical
practice.
By
addressing
obstacles
fostering
innovative
solutions,
this
underscores
potential
ferroptosis-targeting
strategies
revolutionize
treatment
paradigms
Язык: Английский
The Intersections between Neuroscience and Medulloblastoma
Cancer Letters,
Год журнала:
2025,
Номер
unknown, С. 217660 - 217660
Опубликована: Март 1, 2025
Язык: Английский
Glutathione Metabolism in Ferroptosis and Cancer Therapy
Cancer Letters,
Год журнала:
2025,
Номер
unknown, С. 217697 - 217697
Опубликована: Апрель 1, 2025
Язык: Английский
SIK1 promotes ferroptosis resistance in pancreatic cancer via HDAC5-STAT6-SLC7A11 axis
Cancer Letters,
Год журнала:
2025,
Номер
unknown, С. 217726 - 217726
Опубликована: Апрель 1, 2025
Язык: Английский
RPS21 Enhances hepatocellular carcinoma development through GPX4 stabilization
Translational Oncology,
Год журнала:
2024,
Номер
51, С. 102189 - 102189
Опубликована: Ноя. 14, 2024
Язык: Английский
Research progress on ferroptosis regulation in tumor immunity of hepatocellular carcinoma
Journal of Zhejiang University (Medical Sciences),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 1, 2024
Ferroptosis
is
a
form
of
regulated
cell
death,
which
dependent
on
iron
metabolism
imbalance
and
characterized
by
lipid
peroxidation;
it
plays
crucial
role
in
various
pathological
processes.
Studies
have
shown
that
the
occurrence
ferroptosis
closely
associated
with
progression
hepatocellular
carcinoma
(HCC).
involved
regulating
metabolism,
homeostasis,
mitochondrial
redox
processes
HCC.
Additionally,
key
HCC
tumor
immunity
modulating
phenotype
function
immune
cells
microenvironment,
affecting
escape
progression.
Ferroptosis-induced
peroxidation
oxidative
stress
can
promote
polarization
M1
macrophages
enhance
pro-inflammatory
response
tumors,
inhibiting
suppressive
such
as
myeloid-derived
suppressor
Treg
to
disrupt
their
suppression
function.
The
expression
regulation
ferroptosis-related
molecules
GPX4
SLC7A11
not
only
affects
sensitivity
immunotherapy
but
also
directly
influences
activity
survival
effector
T
dendritic
cells,
further
enhancing
or
weakening
host
anti-tumor
response.
Targeting
has
demonstrated
significant
clinical
potential
treatment.
Induction
nanomedicine
molecular
targeting
strategies
kills
enhances
antitumor
responses.
integration
multimodal
therapies
expands
application
cancer
therapy.
This
article
reviews
relationship
between
responses
from
perspective
provide
insights
for
development
based
ferroptosis.
Язык: Английский