Alginate
oligosaccharide
(AOS)
stands
as
a
crucial
carbohydrate-based
biomaterial
in
the
synthesis
of
potential
drugs
and
biological
agents.
In
this
study,
AOS
acylated
derivatives
were
synthesised
by
grafting
maleic
anhydride
(MA)
onto
at
varying
ratios,
their
inhibitory
effects
against
Staphylococcus
aureus
thoroughly
investigated.
Characterization
(AOS-MA-x,
where
x
=
1,
5,
10,
20)
was
conducted
using
Fourier-transformed
infrared
spectroscopy,
1H
nuclear
magnetic
resonance
spectroscopy
X-ray
diffraction,
confirming
successful
these
derivatives.
The
bacteriostatic
activity
AOS-MA
assessed
growth
curves
plate
coating
method,
demonstrating
significant
antibacterial
S.
aureus,
compared
with
AOS.
Among
derivatives,
AOS-MA-20
exhibited
most
potent
selected
for
further
investigation
its
mechanism.
Scanning
electron
microscopy
analysis
revealed
that
treatment
led
to
lysis
rupture
cells,
expelling
intracellular
contents.
Moreover,
disrupted
integrity
cell
wall
membrane
well
impacted
ATPase
some
extent,
ultimately
resulting
bacterial
death.
These
findings
lay
foundational
framework
development
environmentally
friendly
antimicrobial
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Март 19, 2024
Abstract
Background
Traditional
pesticides
are
poorly
water-soluble
and
suffer
from
low
bioavailability.
N
-succinyl
chitosan
(NSCS)
is
a
derivative,
has
been
recently
used
to
encapsulate
hydrophobic
drugs
improve
their
However,
it
remains
challenging
synthesize
of
wide
variety
scale
up
the
production
in
continuous
manner.
Results
A
synthetic
method
for
preparing
nanopesticides
with
polymer
carrier
was
applied.
The
bioactive
molecule
BTL-11
loaded
into
hollow
NSCS
promote
drug
delivery,
solubility
anti-fungal
activity.
synthesized
had
well
controlled
sizes
606
nm
encapsulation
rate
80%.
release
kinetics,
toxicity
activity
were
further
evaluated.
inhibitory
against
Rhizoctonia
solani
(
R.
)
tested
vivo
vitro.
In
trials
revealed
that
excellent
control
efficiency
cultivated
rice
leaf
sheath
79.6
76.5%,
respectively.
By
contrast,
BTL-11@NSCS
NPs,
ability
strongly
released
afforded
significant
efficiencies
85.9
81.1%.
Those
effects
significantly
better
than
agricultural
fungicide
azoxystrobin
(51.5
66.5%).
proposed
mechanism
validated
by
successfully
predicting
synthesis
outcomes.
Conclusions
This
study
demonstrates
promising
biocompatible
carrier,
which
can
enhance
efficacy
pesticides,
synergistically
plant
disease
resistance,
protect
crop
growth,
be
delivery
more
insoluble
pesticides.
Graphical
