Inhibition of TRPA1-like alleviated unfolded protein response and apoptosis by regulating cytoplasmic Ca2+ in Yesso scallop Patinopecten yessoensis under high temperature stress
Frontiers in Marine Science,
Год журнала:
2024,
Номер
11
Опубликована: Май 24, 2024
Transient
receptor
potential
ankyrin
subtype
1
(TRPA1)
is
a
nonselective
cation
channel
protein
typically
forms
ion
channels
that
regulate
intracellular
calcium
homeostasis,
and
can
be
induced
by
temperature
various
chemicals.
In
the
present
study,
involvement
of
Py
TRPA1-like
in
regulating
unfolded
response
(UPR)
apoptosis
Yesso
Scallop
Patinopecten
yessoen
sis
under
high
stress
was
investigated.
The
mRNA
transcripts
were
detected
haemocytes
all
examined
tissues
with
highest
expression
level
mantle.
After
TRPA1
activator
(allyl-isothiocyanate,
AITC)
(25°C)
treatment,
Ca
2+
content
increased
significantly
(
p
<
0.05)
at
3
h,
then
recovered
to
normal
12
GRP78,
IRE1,
ATF6β,
PERK
Caspase-3
haemocytes,
activity
rate
also
upregulated
0.05).
antagonist
(HC-030031)
content,
IRE1
as
well
decreased
compared
control
group
0.05),
while
distribution
showed
no
difference
group.
These
results
collectively
suggest
plays
important
roles
UPR
mediating
influx
scallop
P.
yessoensis
.
Язык: Английский
Identification of transient receptor potential channel genes from the swimming crab, Portunus Trituberculatus, and their expression profiles under acute temperature stress
Yichen Qian,
Qiaoling Yu,
Jun Zhang
и другие.
BMC Genomics,
Год журнала:
2024,
Номер
25(1)
Опубликована: Янв. 17, 2024
Abstract
Background
Temperature
is
an
important
environment
factor
that
critical
to
the
survival
and
growth
of
crustaceans.
However,
mechanisms
by
which
crustaceans
detect
changes
in
temperature
are
still
unclear.
The
transient
receptor
potential
(TRP)
channels
non-selective
cation
well
known
for
properties
sensation.
comprehensive
understandings
on
TRP
as
their
sensing
functions
lacking
Results
In
this
study,
a
total
26
genes
were
identified
swimming
crab,
Portunus
trituberculatus
,
can
be
classified
into
TRPA,
TRPC,
TRPP,
TRPM,
TRPML,
TRPN
TRPV.
Tissue
expression
analysis
revealed
wide
distribution
these
P.
antennules,
neural
tissues,
ovaries
most
commonly
expressed
tissues.
To
investigate
responsiveness
change,
18
TRPs
selected
after
high
low
stress.
results
showed
12
induced
gene
both
groups,
while
3
down-regulated
group,
no
change
either
group.
Conclusions
This
study
characterized
family
explored
involvement
response
Our
will
enhance
overall
understanding
crustacean
possible
functions.
Язык: Английский