Identification of transient receptor potential channel genes from the swimming crab, Portunus Trituberculatus, and their expression profiles under acute temperature stress DOI Creative Commons

Yichen Qian,

Qiaoling Yu,

Jun Zhang

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Jan. 17, 2024

Abstract Background Temperature is an important environment factor that critical to the survival and growth of crustaceans. However, mechanisms by which crustaceans detect changes in temperature are still unclear. The transient receptor potential (TRP) channels non-selective cation well known for properties sensation. comprehensive understandings on TRP as their sensing functions lacking Results In this study, a total 26 genes were identified swimming crab, Portunus trituberculatus , can be classified into TRPA, TRPC, TRPP, TRPM, TRPML, TRPN TRPV. Tissue expression analysis revealed wide distribution these P. antennules, neural tissues, ovaries most commonly expressed tissues. To investigate responsiveness change, 18 TRPs selected after high low stress. results showed 12 induced gene both groups, while 3 down-regulated group, no change either group. Conclusions This study characterized family explored involvement response Our will enhance overall understanding crustacean possible functions.

Language: Английский

Inhibition of TRPA1-like alleviated unfolded protein response and apoptosis by regulating cytoplasmic Ca2+ in Yesso scallop Patinopecten yessoensis under high temperature stress DOI Creative Commons
Xiaoxue Ma, Wenfei Gu, Chuanyan Yang

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: May 24, 2024

Transient receptor potential ankyrin subtype 1 (TRPA1) is a nonselective cation channel protein typically forms ion channels that regulate intracellular calcium homeostasis, and can be induced by temperature various chemicals. In the present study, involvement of Py TRPA1-like in regulating unfolded response (UPR) apoptosis Yesso Scallop Patinopecten yessoen sis under high stress was investigated. The mRNA transcripts were detected haemocytes all examined tissues with highest expression level mantle. After TRPA1 activator (allyl-isothiocyanate, AITC) (25°C) treatment, Ca 2+ content increased significantly ( p < 0.05) at 3 h, then recovered to normal 12 GRP78, IRE1, ATF6β, PERK Caspase-3 haemocytes, activity rate also upregulated 0.05). antagonist (HC-030031) content, IRE1 as well decreased compared control group 0.05), while distribution showed no difference group. These results collectively suggest plays important roles UPR mediating influx scallop P. yessoensis .

Language: Английский

Citations

2
Identification of transient receptor potential channel genes from the swimming crab, Portunus Trituberculatus, and their expression profiles under acute temperature stress DOI Creative Commons

Yichen Qian,

Qiaoling Yu,

Jun Zhang

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Jan. 17, 2024

Abstract Background Temperature is an important environment factor that critical to the survival and growth of crustaceans. However, mechanisms by which crustaceans detect changes in temperature are still unclear. The transient receptor potential (TRP) channels non-selective cation well known for properties sensation. comprehensive understandings on TRP as their sensing functions lacking Results In this study, a total 26 genes were identified swimming crab, Portunus trituberculatus , can be classified into TRPA, TRPC, TRPP, TRPM, TRPML, TRPN TRPV. Tissue expression analysis revealed wide distribution these P. antennules, neural tissues, ovaries most commonly expressed tissues. To investigate responsiveness change, 18 TRPs selected after high low stress. results showed 12 induced gene both groups, while 3 down-regulated group, no change either group. Conclusions This study characterized family explored involvement response Our will enhance overall understanding crustacean possible functions.

Language: Английский

Citations

1