Piperine-coated zinc oxide nanoparticles target biofilms and induce oral cancer apoptosis via BCl-2/BAX/P53 pathway

BMC Oral Health, Год журнала: 2024, Номер 24(1)

Опубликована: Июнь 21, 2024

Abstract Background Dental pathogens play a crucial role in oral health issues, including tooth decay, gum disease, and infections, recent research suggests link between these cancer initiation progression. Innovative therapeutic approaches are needed due to antibiotic resistance concerns treatment limitations. Methods We synthesized analyzed piperine-coated zinc oxide nanoparticles (ZnO-PIP NPs) using UV spectroscopy, SEM, XRD, FTIR, EDAX. Antioxidant antimicrobial effectiveness were evaluated through DPPH, ABTS, MIC assays, while the anticancer properties assessed on KB squamous carcinoma cells. Results ZnO-PIP NPs exhibited significant antioxidant activity of 50 µg/mL against dental pathogens, indicating strong properties. Interaction analysis revealed high binding affinity with pathogens. showed dose-dependent cells, upregulating apoptotic genes BCL2, BAX, P53. Conclusions This approach offers multifaceted solution combatting both infections cancer, showcasing their potential for advancement healthcare. It is essential acknowledge limitations challenges associated use ZnO clinical applications. These may include regarding nanoparticle toxicity, biocompatibility, long-term safety. Further rigorous testing warranted address issues ensure safe effective translation into practice.

Язык: Английский

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Multifunctional curcumin mediated zinc oxide nanoparticle enhancing biofilm inhibition and targeting apoptotic specific pathway in oral squamous carcinoma cells

Molecular Biology Reports, Год журнала: 2024, Номер 51(1)

Опубликована: Март 15, 2024

Язык: Английский

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The Antioxidant, Antibacterial and Cell-Protective Properties of Bioactive Compounds Extracted from Rowanberry (Sorbus aucuparia L.) Fruits In Vitro

Plants, Год журнала: 2024, Номер 13(4), С. 538 - 538

Опубликована: Фев. 16, 2024

Considering that Sorbus aucuparia fruits have been underutilized despite their tremendous potential, this study aimed to correlate the in vitro antioxidant, antibacterial and cell-protective abilities of fruit extracts derived from Romanian cultivars with phytochemical composition. Therefore, following preparation ethanolic carotenoid extracts, screening was performed using UV–Vis HPLC-DAD-ESI-MS methods. The antioxidant activity analyzed DPPH FRAP tests. As results revealed high contents bioactive compounds (polyphenols 1.11 mg GAE/g DM, flavonoids 430.06 µg QE/g DM carotenoids 95.68 µg/g DM) an important antiradical action (DPPH 24.51 mg/mL 0.016 µM TE/mL), we chose further examine fruits’ biological properties. capacity assessed employing agar well diffusion broth microdilution techniques, displaying intense against MSSA, MRSA Enterococcus faecalis, but also E. coli Pseudomonas aeruginosa. on gentamicin-stressed renal cells, through MTT Annexin V-FITC assays. Importantly, a significant increase viability registered stressed cells extract administration low doses; nevertheless, noticed decline when exposed elevated concentrations, potentially due cumulative actions gentamicin. These findings offer novel light cultivars, as ability cell injury.

Язык: Английский

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Ophthalmic Intervention of Naringenin Decreases Vascular Endothelial Growth Factor by Counteracting Oxidative Stress and Cellular Damage in In Vivo Zebrafish

Molecules, Год журнала: 2023, Номер 28(14), С. 5350 - 5350

Опубликована: Июль 12, 2023

Diabetes Mellitus is a metabolic disease that leads to microvascular complications like Diabetic retinopathy (DR), major cause of blindness worldwide. Current medications for DR are expensive and report multiple side effects; therefore, an alternative medication alleviates the condition required. An interventional approach targeting vascular endothelial growth factor (VEGF) remains treatment strategy DR. Anti-VEGF medicines being investigated as main therapy managing vision-threatening DR, such diabetic macular oedema. Therefore, this study effect flavonoid naringenin (NG) from citrus fruits on inhibiting early in zebrafish. When exposed 130 mM glucose, zebrafish larvae developed hyperglycaemic accompanied by oxidative stress, cellular damage, lipid peroxidation. Similarly, when adult were 4% Glucose, high glucose levels observed ocular region massive destruction retinal membrane. High upregulated expression VEGF. In comparison, co-exposure NG inhibited stress damage restored glutathione larvae. regressed along with inhibition degeneration retina normalized overexpression VEGF promising treating intervention could alleviate domestication medicine ophthalmic research.

Язык: Английский

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Treatment of diabetic complications: do flavonoids holds the keys?

Critical Reviews in Food Science and Nutrition, Год журнала: 2023, Номер 64(30), С. 11091 - 11112

Опубликована: Июль 12, 2023

Diabetes mellitus (DM) is an endocrinological disorder in which blood sugar levels get elevated and if unmanaged, it leads to several critical complications. Existing therapies or drugs are not able attain absolute control of DM. Moreover, associated side/adverse effects with pharmacotherapy further worsen the Quality life patients. Present review focused on therapeutical potential flavonoids management diabetes diabetic Plenteous literature has established significant treatment A number found be effective only but progression complication was also attenuated use flavonoids. SAR studies some indicated that efficacy increased a change functional group clinical trials into action investigate therapeutic as first-line adjuvants for complications.. Owing their diverse mechanism action, safety, may conscripted candidate

Язык: Английский

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Daidzein ameliorates nonmotor symptoms of manganese‐induced Parkinsonism in zebrafish model: Behavioural and biochemical approach

British Journal of Pharmacology, Год журнала: 2024, Номер 181(16), С. 2947 - 2963

Опубликована: Апрель 28, 2024

Abstract Background and Purpose Parkinson's disease (PD) is a prevalent neurodegenerative movement disorder characterized by motor dysfunction. Environmental factors, especially manganese (Mn), contribute significantly to PD. Existing therapies are focused on coordination, whereas nonmotor features such as neuropsychiatric symptoms often neglected. Daidzein (DZ), phytoestrogen, has piqued interest due its antioxidant, anti‐inflammatory, anxiolytic properties. Therefore, we anticipate that DZ might be an effective drug alleviate the of Mn‐induced Parkinsonism. Experimental Approach Naïve zebrafish were exposed 2 mM Mn for 21 days intervened with DZ. Nonmotor anxiety, social behaviour, olfactory function assessed. Acetylcholinesterase (AChE) activity antioxidant enzyme status measured from brain tissue through biochemical assays. Dopamine levels histology performed elucidate neuroprotective mechanism Key Results exhibited effects in novel environment also improved intra inter fish behaviour. response amino acid stimuli reduced oxidative stress AChE prevented neuronal damage. increased DA level brain, collectively contributing neuroprotection. Conclusion Implications demonstrated promising effect alleviating anxiety dysfunction, mitigation cellular These findings underscore therapeutic potential addressing neurotoxicity induced heavy metals, particularly context

Язык: Английский

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Bisphenol A-Induced Ovarian Damage Countered by Luteolin: Experiments in In Vitro CHO Cells and In Vivo PCOS Phenotype Zebrafish

Tissue and Cell, Год журнала: 2024, Номер 91, С. 102532 - 102532

Опубликована: Авг. 24, 2024

Язык: Английский

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β-cells regeneration by WL15 of cysteine and glycine-rich protein 2 which reduces alloxan induced β-cell dysfunction and oxidative stress through phosphoenolpyruvate carboxykinase and insulin pathway in zebrafish in-vivo larval model

Molecular Biology Reports, Год журнала: 2022, Номер 49(12), С. 11867 - 11879

Опубликована: Окт. 12, 2022

Язык: Английский

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Integrated Metabolomics and Metagenomics Unveiled Biomarkers of Antioxidant Potential in Fermented Brewer’s Grains

Antioxidants, Год журнала: 2024, Номер 13(7), С. 872 - 872

Опубликована: Июль 20, 2024

About one-third of the global food supply is wasted. Brewers’ spent grain (BSG), being produced in enormous amounts by brewery industry, possesses an eminence nutritional profile, yet its recycling often neglected for multiple reasons. We employed integrated metagenomics and metabolomics techniques to assess effects enzyme treatments Lactobacillus fermentation on antioxidant capacity BSG. The biotreated BSG revealed improved capability, as evidenced significantly increased (p < 0.05) radical scavenging activity flavonoid polyphenol content. Untargeted that led prominent synthesis 15 novel peptides, well higher enrichment isoflavonoid phenylpropanoid biosynthesis pathways. correlation analysis demonstrated Lactiplantibacillus plantarum exhibited strong with aucubin carbohydrate-active enzymes, namely, glycoside hydrolases 25, glycosyl transferases 5, carbohydrate esterases 9. fermented has potential applications industry a culture medium, functional component human consumption, bioactive feed ingredient animals.

Язык: Английский

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Graphene oxide decorated daidzein as an oral drug to ameliorate the oxidative stress and glucocorticoid-induced osteoporosis in vivo zebrafish model

Journal of Drug Delivery Science and Technology, Год журнала: 2023, Номер 81, С. 104278 - 104278

Опубликована: Фев. 16, 2023

Язык: Английский

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