Methods and applications for single-cell and spatial multi-omics

Nature Reviews Genetics, Год журнала: 2023, Номер 24(8), С. 494 - 515

Опубликована: Март 2, 2023

Язык: Английский

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Hallmarks of transcriptional intratumour heterogeneity across a thousand tumours

Nature, Год журнала: 2023, Номер 618(7965), С. 598 - 606

Опубликована: Май 31, 2023

Язык: Английский

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Macrophages and microglia in glioblastoma: heterogeneity, plasticity, and therapy

Journal of Clinical Investigation, Год журнала: 2023, Номер 133(1)

Опубликована: Янв. 2, 2023

Glioblastoma (GBM) is the most aggressive tumor in central nervous system and contains a highly immunosuppressive microenvironment (TME). Tumor-associated macrophages microglia (TAMs) are dominant population of immune cells GBM TME that contribute to hallmarks, including immunosuppression. The understanding TAMs has been limited by lack powerful tools characterize them. However, recent progress on single-cell technologies offers an opportunity precisely at level identify new TAM subpopulations with specific tumor-modulatory functions GBM. In this Review, we discuss heterogeneity plasticity summarize current TAM-targeted therapeutic potential We anticipate use followed functional studies will accelerate development novel effective therapeutics for patients.

Язык: Английский

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Primary brain tumours in adults

The Lancet, Год журнала: 2023, Номер 402(10412), С. 1564 - 1579

Опубликована: Сен. 19, 2023

Язык: Английский

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Single-cell and spatial transcriptomics: deciphering brain complexity in health and disease

Nature Reviews Neurology, Год журнала: 2023, Номер 19(6), С. 346 - 362

Опубликована: Май 17, 2023

Язык: Английский

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Spatially resolved multi-omics highlights cell-specific metabolic remodeling and interactions in gastric cancer

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Май 10, 2023

Abstract Mapping tumor metabolic remodeling and their spatial crosstalk with surrounding non-tumor cells can fundamentally improve our understanding of biology, facilitates the designing advanced therapeutic strategies. Here, we present an integration mass spectrometry imaging-based metabolomics lipidomics microarray-based transcriptomics to hierarchically visualize intratumor heterogeneity cell interactions in same gastric cancer sample. Tumor-associated reprogramming is imaged at metabolic-transcriptional levels, maker metabolites, lipids, genes are connected pathways colocalized heterogeneous tissues. Integrated data from multi-omics approaches coherently identify types distributions within complex microenvironment, immune cell-dominated “tumor-normal interface” region where contact adjacent tissues characterized distinct transcriptional signatures significant immunometabolic alterations. Our approach for mapping tissue molecular architecture provides highly integrated picture heterogeneity, transform metabolism systemic level.

Язык: Английский

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Immune checkpoint blockade in glioblastoma: from tumor heterogeneity to personalized treatment

Journal of Clinical Investigation, Год журнала: 2023, Номер 133(2)

Опубликована: Янв. 16, 2023

Immune checkpoint blockade (ICB) has revolutionized modern cancer therapy, arousing great interest in the neuro-oncology community. While several reports show that subsets of patients with glioma exhibit durable responses to immunotherapy, efficacy this treatment not been observed for unselected patient populations, preventing its broad clinical implementation gliomas and glioblastoma (GBM). To exploit maximum therapeutic potential ICB glioma, understanding different aspects glioma-related tumor immune is critical importance. In Review, we discuss contributing factors distinguish who may benefit from ICB. Specifically, (a) complex interaction between microenvironment cells as a influence on immunotherapy responses; (b) promising biomarkers inhibitors; (c) contributions peripheral responses.

Язык: Английский

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To metabolomics and beyond: a technological portfolio to investigate cancer metabolism

Signal Transduction and Targeted Therapy, Год журнала: 2023, Номер 8(1)

Опубликована: Март 22, 2023

Abstract Tumour cells have exquisite flexibility in reprogramming their metabolism order to support tumour initiation, progression, metastasis and resistance therapies. These reprogrammed activities include a complete rewiring of the bioenergetic, biosynthetic redox status sustain increased energetic demand cells. Over last decades, cancer field has seen an explosion new biochemical technologies giving more tools than ever before navigate this complexity. Within cell or tissue, metabolites constitute direct signature molecular phenotype thus profiling concrete clinical applications oncology. Metabolomics fluxomics, are key technological approaches that mainly revolutionized enabling researchers both qualitative mechanistic model cancer. Furthermore, upgrade from bulk single-cell analysis provided unprecedented opportunity investigate biology at cellular resolution allowing depth quantitative complex heterogenous diseases. More recently, advent functional genomic screening allowed identification pathways, processes, biomarkers novel therapeutic targets concert with other allow patient stratification treatment regimens. This review is intended be guide for metabolism, highlighting current emerging technologies, emphasizing advantages, disadvantages potential leading development innovative anti-cancer

Язык: Английский

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Hypoxic niches attract and sequester tumor-associated macrophages and cytotoxic T cells and reprogram them for immunosuppression

Immunity, Год журнала: 2023, Номер 56(8), С. 1825 - 1843.e6

Опубликована: Июль 13, 2023

Язык: Английский

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Emerging therapies in cancer metabolism

Cell Metabolism, Год журнала: 2023, Номер 35(8), С. 1283 - 1303

Опубликована: Авг. 1, 2023

Язык: Английский

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