Biofabrication,
Год журнала:
2024,
Номер
17(1), С. 015036 - 015036
Опубликована: Дек. 10, 2024
Abstract
The
fibroblast-myofibroblast
transition
marked
by
extracellular
matrix
(ECM)
secretion
and
contraction
of
actomyosin-based
stress
fibers,
plays
central
roles
in
the
wound
healing
process.
This
work
aims
to
utilize
cell
membrane-based
nanoplatform
improve
outcomes
dysregulated
healing.
membranes
myofibroblasts
isolated
from
mouse
skin
are
used
as
camouflage
for
gold
nanoparticles
loaded
with
IL-4
cytokine.
membrane-modified
show
effective
situ
clearance
bacterial
infection,
act
activator
IL-4Rα
signaling
pathway
induce
pro-inflammatory
M1
macrophages
into
anti-inflammatory
M2
phenotype.
Thus,
poor
bacteria-clearance
non-stop
inflammation
refractory
wounds
improved
accelerated.
Furthermore,
releases
myofibroblast
propel
primitive
fibroblasts
toward
an
epigenetic
manner.
Matrix-production,
vascularization,
epithelial
regeneration
then
initiated,
leading
satisfactory
closure.
Our
study
devises
a
new
strategy
activating
under
prolonged
continuous
exposure
fibrotic
environment,
develops
promising
biomimetic
treatment
chronic
ABSTRACT
Cancer‐associated
fibroblasts
(CAFs)
are
key
components
of
the
tumor
microenvironment
(TME).
Given
their
various
roles
in
progression
and
treatment
resistance,
CAFs
promising
therapeutic
targets
cancer.
The
elimination
tumor‐promoting
has
been
investigated
animal
models
to
determine
whether
it
effectively
suppresses
growth.
Based
on
recent
evidence,
several
simple
strategies
have
proposed
eliminate
attenuate
these
features.
In
addition,
attention
focused
critical
role
that
play
immunosuppressive
TME.
Therefore,
functional
reprogramming
combination
with
immune
checkpoint
inhibitors
also
as
a
possible
approach.
However,
although
potential
widely
characterized,
plasticity
heterogeneity
complicate
understanding
properties
present
difficulties
for
clinical
application.
Moreover,
identification
tumor‐suppressive
highlights
necessity
development
approaches
can
distinguish
switch
between
an
appropriate
manner.
this
review,
we
introduce
origins
diversity
CAFs,
cancer,
current
aimed
at
targeting
including
ongoing
evaluations.
ACS Nano,
Год журнала:
2025,
Номер
19(1), С. 580 - 599
Опубликована: Янв. 1, 2025
Idiopathic
pulmonary
fibrosis
(IPF)
is
characterized
by
persistent
tissue
injury,
dysregulated
wound
healing,
and
extracellular
matrix
(ECM)
deposition
myofibroblasts
(MFs)
through
the
fibroblast-to-myofibroblast
transition
(FMT).
Implicit
in
FMT
process
are
changes
ECM
cellular
topology,
but
their
relationship
with
lung
fibroblast
phenotype
has
not
been
explored.
We
engineered
topological
mimetics
of
alignment
cues
(anisotropy/isotropy)
using
decellularized
micropattern
arrays
investigated
effects
topology
on
fates
MRC-5
fibroblasts.
found
that
isotropic
cells
presented
cytoskeleton,
increased
cell–cell
adhesions
a
multicellular
architecture
overlap,
actin–myosin
development,
enhanced
focal
adhesion
cell
junction
random
alignment.
Besides,
anisotropic
fibroblasts
were
activated
into
regular
an
remodeling
profile.
In
contrast,
developed
highly
invasive
expressing
molecules,
including
CD274/programmed
death-ligand
1
(PD-L1),
communication
network
factor
2
(CCN2)/connective
growth
(CTGF),
hyaluronan
synthase
(HAS2),
semaphorin
7A
(SEMA7A),
downregulated
genes.
Moreover,
also
showed
higher
expressions
Ki-67
cyclin
D1
(CCND1),
resistance
to
apoptosis/senescence,
decreased
autophagy.
The
regulated
heterogeneity
resulted
positive
feedback
between
structure,
which
may
aggravate
lead
priming
malignant
microenvironment
during
carcinogenesis.
Using
versatile
platform
array,
we
can
only
visualize
interaction
mechanism
select
potential
clinical
targets
for
diagnosis
therapeutics.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 9, 2025
Thyroid
cancer
progression
from
curable
well-differentiated
thyroid
carcinoma
to
highly
lethal
anaplastic
is
distinguished
by
tumor
cell
de-differentiation
and
recruitment
of
a
robust
stromal
infiltrate.
Combining
an
integrated
single-cell
sequencing
atlas
with
spatial
transcriptomics
bulk
RNA-sequencing,
we
define
subpopulations
tumor-stromal
cross-talk
occurring
across
the
histologic
mutational
spectrum
cancer.
We
identify
distinct
inflammatory
myofibroblastic
cancer-associated
fibroblast
(iCAF
myCAF)
populations
perivascular-like
populations.
The
myCAF
population
only
found
in
malignant
samples
associated
invasion,
BRAF
V600E
mutation,
lymph
node
metastasis,
disease
progression.
Tumor-adjacent
myCAFs
abut
invasive
cells
partial
epithelial-to-mesenchymal
phenotype.
Tumor-distant
iCAFs
infiltrate
autoimmune
lesions
tumors.
In
summary,
our
study
provides
subtypes
characterization
at
sites
invasion
de-differentiation,
defining
reorganization
central
Theranostics,
Год журнала:
2025,
Номер
15(8), С. 3332 - 3344
Опубликована: Фев. 18, 2025
Antigen-presenting
fibroblasts
are
a
newly
recognized
subset
that
challenges
the
traditional
view
of
these
cells
as
mere
structural
components.
Under
pathological
or
environmental
stimuli,
acquire
antigen-presenting
capabilities
through
expression
MHC-II
molecules
and
co-stimulatory
factors,
enabling
them
to
interact
with
T
modulate
immune
responses.
These
specialized
have
been
identified
across
various
tissues
diseases,
where
they
play
context-dependent
roles,
either
amplifying
dysregulation
contributing
homeostasis.
This
review
synthesizes
recent
advances
in
understanding
origins,
activation,
functions
fibroblasts.
It
highlights
their
role
promoting
pathogenic
responses
offering
therapeutic
opportunities
targeted
modulation.
Advancing
our
holds
great
promise
for
developing
innovative
approaches
modulation
therapy
range
diseases.
