Molecules,
Год журнала:
2023,
Номер
28(18), С. 6565 - 6565
Опубликована: Сен. 11, 2023
The
drive
to
achieve
ultrasensitive
target
detection
with
exceptional
efficiency
and
accuracy
requires
the
advancement
of
immunoassays.
Optical
immunoassays
have
demonstrated
significant
potential
in
clinical
diagnosis,
food
safety,
environmental
protection,
other
fields.
Through
innovative
feasible
combination
enzyme
catalysis
optical
immunoassays,
notable
progress
has
been
made
enhancing
analytical
performances.
Among
kinds
reporter
enzymes,
alkaline
phosphatase
(ALP)
stands
out
due
its
high
catalytic
activity,
elevated
turnover
number,
broad
substrate
specificity,
rendering
it
an
excellent
candidate
for
development
various
This
review
provides
a
systematic
evaluation
advancements
by
employing
ALP
as
signal
label,
encompassing
fluorescence,
colorimetry,
chemiluminescence,
surface-enhanced
Raman
scattering.
Particular
emphasis
is
placed
on
fundamental
amplification
strategies
employed
ALP-linked
Furthermore,
this
work
briefly
discusses
proposed
solutions
challenges
that
need
be
addressed
further
enhance
performances
Analytical Chemistry,
Год журнала:
2023,
Номер
95(4), С. 2356 - 2365
Опубликована: Янв. 16, 2023
Alkaline
phosphatase
(ALP)
and
interleukin-1beta
(IL-1β)
are
crucial
salivary
biomarkers
for
the
diagnosis
of
periodontal
disease
that
harms
tissue
along
with
tooth
loss.
However,
there
has
been
no
way
sensitive
portable
detection
both
in
saliva
multivariate
signal
readout.
In
this
work,
we
design
multicolorimetric
ALP
IL-1β
sensing
platform
based
on
geometrical
transformation
silver
nanoplate
transducer.
By
utilizing
enzymatic
activity
dephosphorylates
p-aminophenol
phosphate
(p-APP)
to
(p-AP),
localized
surface
plasmon
resonance
properties
vary
show
a
distinct
color
change
from
blue
yellow
controlled
seed
triangular
hexagonal,
rounded
pentagonal,
spherical
shape.
The
multicolor
sensor
shows
an
range
0–25
U/L
limit
(LOD)
0.0011
U/L,
which
is
lowest
LOD
demonstrated
date
state-of-the-art
sensor.
Furthermore,
integrate
conventional
ELISA
detect
signaling
it
exhibits
linear
0–250
pg/mL
0.066
pg/mL,
2
orders
magnitude
lower
than
monochromic
(LOD
3.8
pg/mL).
high
selectivity
recovery
100.9%
real
human
proving
its
reliability
suitability
readily
accessible
RSC Advances,
Год журнала:
2023,
Номер
13(24), С. 16413 - 16452
Опубликована: Янв. 1, 2023
Heterocycles
are
a
class
of
compounds
that
have
been
found
to
be
potent
inhibitors
alkaline
phosphatase
(AP),
an
enzyme
plays
critical
role
in
various
physiological
processes
such
as
bone
metabolism,
cell
growth
and
differentiation,
has
linked
several
diseases
cancer
osteoporosis.
AP
is
widely
distributed
enzyme,
its
inhibition
considered
therapeutic
strategy
for
the
treatment
these
diseases.
Heterocyclic
inhibit
by
binding
active
site
thereby
inhibiting
activity.
imidazoles,
pyrazoles,
pyridines
studied
potential
therapeutics
cancer,
osteoporosis,
other
However,
development
more
selective
can
used
ongoing
area
research.
Additionally,
study
mechanism
action
heterocyclic
research,
which
could
lead
identification
new
targets
strategies.
The
known
functions
present
multiple
tissues
organs
throughout
body.
This
article
presents
overview
different
types
isoforms,
their
distribution,
roles.
It
also
discusses
structure
AP,
including
hydrolysis
phosphate
groups.
Furthermore,
importance
clinical
marker
liver
disease,
disorders,
emphasized,
well
use
diagnosis
rare
inherited
disorders
hypophosphatasia.
applications
explored.
objective
this
literature
review
examine
function
conditions
diseases,
analyze
structure-activity
relationships
recently
reported
inhibitors.
summarizes
relationship
(SAR)
SAR
studies
revealed
presence
ring,
particularly
pyridine,
pyrimidine,
or
pyrazole
molecule
essential
inhibitory
substitution
pattern
stereochemistry
ring
play
crucial
determining
potency
inhibitor.
Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
62(29)
Опубликована: Май 16, 2023
In
this
article,
we
report
a
novel
targeting
strategy
involving
the
combination
of
an
enzyme-instructed
self-assembly
(EISA)
moiety
and
strained
cycloalkyne
to
generate
large
accumulation
bioorthogonal
sites
in
cancer
cells.
These
can
serve
as
activation
triggers
different
regions
for
transition
metal-based
probes,
which
are
new
ruthenium(II)
complexes
carrying
tetrazine
unit
controllable
phosphorescence
singlet
oxygen
generation.
Importantly,
environment-sensitive
emission
be
further
enhanced
hydrophobic
offered
by
supramolecular
assemblies,
is
highly
advantageous
biological
imaging.
Additionally,
(photo)cytotoxicity
assemblies
containing
was
investigated,
results
illustrate
that
cellular
localization
(extracellular
intracellular)
imposes
profound
impact
on
efficiencies
photosensitizers.
International Journal of Electrochemical Science,
Год журнала:
2023,
Номер
18(12), С. 100395 - 100395
Опубликована: Ноя. 1, 2023
Peptides
have
attracted
great
interest
in
the
construction
of
electrochemical
biosensors
fields
biomedicine,
disease
diagnosis,
and
environmental
monitoring.
They
exhibit
advantages
high
affinity
specificity,
chemical
diversity
building
block
amino
acids,
standard
synthesis
scheme.
Versatile
peptide-based
been
developed
for
detection
various
targets,
including
metal
ions,
enzymes,
proteins,
exosomes,
cells,
bacteria.
their
self-assembled
nanostructures
can
be
used
as
recognition
elements,
enzyme
substrates,
electrode
materials,
signal
labels.
Different
functional
species,
electroactive
small
molecules,
nanomaterials
coupled
with
peptides
direct
conversion
binding/modification
events
into
measurable
signals.
Herein,
we
systematically
summarize
progress
according
to
difference
peptide
function
target
type.
The
peptide-functionalized
molecules
amplification
strategies
improving
sensitivity
were
discussed.
Molecules,
Год журнала:
2024,
Номер
29(12), С. 2796 - 2796
Опубликована: Июнь 12, 2024
Enzyme-linked
electrochemical
immunosensors
have
attracted
considerable
attention
for
the
sensitive
and
selective
detection
of
various
targets
in
clinical
diagnosis,
food
quality
control,
environmental
analysis.
In
order
to
improve
performances
conventional
immunoassays,
significant
efforts
been
made
couple
enzyme-linked
or
nanozyme-based
catalysis
redox
cycling
signal
amplification.
The
current
review
summarizes
recent
advances
development
enzyme-
with
special
features
reactions
their
synergistic
functions
amplification
are
discussed.
Additionally,
challenges
future
directions
addressed.
Molecules,
Год журнала:
2024,
Номер
29(19), С. 4551 - 4551
Опубликована: Сен. 25, 2024
Immunoassays
have
been
widely
used
for
the
determination
of
various
analytes
in
fields
disease
diagnosis,
food
safety,
and
environmental
monitoring.
Dual-signal
immunoassays
are
now
advanced
integrated
detection
technologies
with
excellent
self-correction
self-validation
capabilities.
In
this
work,
we
summarize
recent
advances
development
optical
electrochemical
dual-signal
immunoassays,
including
colorimetric,
fluorescence,
surface-enhanced
Raman
spectroscopy
(SERS),
electrochemical,
electrochemiluminescence,
photoelectrochemical
methods.
This
review
particularly
emphasizes
working
principle
diverse
utilization
dual-functional
molecules
nanomaterials.
It
also
outlines
challenges
prospects
future
research
on
immunoassays.
