ACS Materials Letters,
Journal Year:
2024,
Volume and Issue:
6(3), P. 1059 - 1068
Published: Feb. 22, 2024
Drug
toxicity
and
related
drug-induced
liver
injury
(DILI)
have
become
major
biosafety
issues.
Enzyme-activated
fluorescent
probes
been
demonstrated
as
a
powerful
tool
for
the
diagnosis
of
DILI;
however,
they
suffer
from
diffusive
signal
dilution
interference
with
other
organs,
thus
leading
to
misassessment
drug
inaccurate
diagnosis.
Alkaline
phosphatase
(ALP)
is
an
important
biomarker,
alterations
in
enzyme
level
are
tightly
correlated
severity
damage.
Herein,
enzyme-activated
intramolecular
hydrogen
bond
(IMHB)
enhanced
probe
(TPEG-P)
was
developed
ALP
detection
cells
mice
models
DILI.
Differing
previously
reported
intermolecular
charge
transfer
(ICT)
or
excited-state
proton
(ESIPT)
mechanisms,
TPEG-P
enables
precise
recognition
imaging
only
through
activation
bonds
could
situ
sensitively
detect
varying
degrees
caused
by
toxicity.
This
IMHB
strategy
will
advance
development
bioimaging
future.
Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(29)
Published: May 16, 2023
In
this
article,
we
report
a
novel
targeting
strategy
involving
the
combination
of
an
enzyme-instructed
self-assembly
(EISA)
moiety
and
strained
cycloalkyne
to
generate
large
accumulation
bioorthogonal
sites
in
cancer
cells.
These
can
serve
as
activation
triggers
different
regions
for
transition
metal-based
probes,
which
are
new
ruthenium(II)
complexes
carrying
tetrazine
unit
controllable
phosphorescence
singlet
oxygen
generation.
Importantly,
environment-sensitive
emission
be
further
enhanced
hydrophobic
offered
by
supramolecular
assemblies,
is
highly
advantageous
biological
imaging.
Additionally,
(photo)cytotoxicity
assemblies
containing
was
investigated,
results
illustrate
that
cellular
localization
(extracellular
intracellular)
imposes
profound
impact
on
efficiencies
photosensitizers.
International Journal of Electrochemical Science,
Journal Year:
2023,
Volume and Issue:
18(12), P. 100395 - 100395
Published: Nov. 1, 2023
Peptides
have
attracted
great
interest
in
the
construction
of
electrochemical
biosensors
fields
biomedicine,
disease
diagnosis,
and
environmental
monitoring.
They
exhibit
advantages
high
affinity
specificity,
chemical
diversity
building
block
amino
acids,
standard
synthesis
scheme.
Versatile
peptide-based
been
developed
for
detection
various
targets,
including
metal
ions,
enzymes,
proteins,
exosomes,
cells,
bacteria.
their
self-assembled
nanostructures
can
be
used
as
recognition
elements,
enzyme
substrates,
electrode
materials,
signal
labels.
Different
functional
species,
electroactive
small
molecules,
nanomaterials
coupled
with
peptides
direct
conversion
binding/modification
events
into
measurable
signals.
Herein,
we
systematically
summarize
progress
according
to
difference
peptide
function
target
type.
The
peptide-functionalized
molecules
amplification
strategies
improving
sensitivity
were
discussed.
ACS Nano,
Journal Year:
2023,
Volume and Issue:
18(1), P. 1084 - 1097
Published: Dec. 27, 2023
Water
instability
and
sensing
homogeneity
are
the
Achilles'
heel
of
CsPbX3
NPs
in
biological
fluids
application.
This
work
reports
preparation
Mn2+:CsPbCl3@SiO2
yolk–shell
nanoparticles
(YSNPs)
aqueous
solutions
created
through
integration
ligand,
surface,
crystal
engineering
strategies.
The
SN2
reaction
between
4-chlorobutyric
acid
(CBA)
oleylamine
(OAm)
yields
a
zwitterionic
ligand
that
facilitates
dispersion
YSNPs
water,
while
robust
SiO2
shell
enhances
their
overall
stability.
Besides,
Mn2+
doping
not
only
introduces
second
emission
center
but
also
enables
potential
postsynthetic
designability,
leading
to
switching
from
MnO2@YSNPs
with
excellent
oxidase
(OXD)-like
activity.
Theoretical
calculations
reveal
electron
transfer
CsPbCl3
situ
MnO2
adsorption–desorption
process
3,3′,5,5′-tetramethylbenzidine
(TMB)
synergistically
amplify
OXD-like
In
presence
ascorbic
(AA),
Mn4+
(fluorescent
nanozyme)
is
reduced
dissociated,
thereby
inhibiting
activity
triggering
fluorescence
"turn-on/off",
i.e.,
dual-mode
recognition.
Finally,
biomarker
reporting
platform
based
on
fluorescent
nanozyme
constructed
AA
as
reporter
molecule,
accurate
detection
human
serum
alkaline
phosphatase
(ALP)
realized,
demonstrating
vast
perovskites
biosensing.
Molecules,
Journal Year:
2024,
Volume and Issue:
29(12), P. 2796 - 2796
Published: June 12, 2024
Enzyme-linked
electrochemical
immunosensors
have
attracted
considerable
attention
for
the
sensitive
and
selective
detection
of
various
targets
in
clinical
diagnosis,
food
quality
control,
environmental
analysis.
In
order
to
improve
performances
conventional
immunoassays,
significant
efforts
been
made
couple
enzyme-linked
or
nanozyme-based
catalysis
redox
cycling
signal
amplification.
The
current
review
summarizes
recent
advances
development
enzyme-
with
special
features
reactions
their
synergistic
functions
amplification
are
discussed.
Additionally,
challenges
future
directions
addressed.
Molecules,
Journal Year:
2024,
Volume and Issue:
29(19), P. 4551 - 4551
Published: Sept. 25, 2024
Immunoassays
have
been
widely
used
for
the
determination
of
various
analytes
in
fields
disease
diagnosis,
food
safety,
and
environmental
monitoring.
Dual-signal
immunoassays
are
now
advanced
integrated
detection
technologies
with
excellent
self-correction
self-validation
capabilities.
In
this
work,
we
summarize
recent
advances
development
optical
electrochemical
dual-signal
immunoassays,
including
colorimetric,
fluorescence,
surface-enhanced
Raman
spectroscopy
(SERS),
electrochemical,
electrochemiluminescence,
photoelectrochemical
methods.
This
review
particularly
emphasizes
working
principle
diverse
utilization
dual-functional
molecules
nanomaterials.
It
also
outlines
challenges
prospects
future
research
on
immunoassays.
Analytical Methods,
Journal Year:
2024,
Volume and Issue:
16(7), P. 1093 - 1101
Published: Jan. 1, 2024
Recent
strides
in
nanotechnology
have
given
rise
to
nanozymes,
nanomaterials
designed
emulate
enzymatic
functions.
Despite
their
promise,
challenges
such
as
batch-to-batch
variability
and
limited
atomic
utilization
persist.
This
study
introduces
Pt(Glu)2,
a
platinum
glutamic
acid
complex,
versatile
small-molecule
peroxidase
mimic.
Synthesized
through
straightforward
method,
Pt(Glu)2
exhibits
robust
catalytic
activity
stability.
Steady-state
kinetics
reveal
lower
Km
value
compared
that
of
natural
enzymes,
signifying
strong
substrate
affinity.
was
explored
for
controllable
chemical
modification
integration
into
cascade
reactions
with
surpassing
other
nanomaterials.
Its
facile
synthesis
seamless
enhance
beyond
the
capabilities
nanozymes.
In
biosensing
applications,
enabled
simultaneous
detection
cholesterol
alkaline
phosphatase
human
serum
high
selectivity
sensitivity.
These
findings
illustrate
potential
small
molecule
mimetics
catalysis
biosensing,
paving
way
broader
applications.
